Molecular cloning and expression analysis of ubiquitin-conjugating enzyme E2-D2 in roughskin sculpin,<em>Trachidermus Fasciatus</em>

Chen Xuezhao; Zhang Lei; Yu Shanshan; Bi Caihong; Liu Chunying; Zhu Qian

doi:10.3969/j.issn.0253-4193.2015.10.013

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Chen Xuezhao, Zhang Lei, Yu Shanshan, Bi Caihong, Liu Chunying, Zhu Qian. Molecular cloning and expression analysis of ubiquitin-conjugating enzyme E2-D2 in roughskin sculpin,Trachidermus Fasciatus[J]. Haiyang Xuebao, 2015, 37(10): 133-140. doi: 10.3969/j.issn.0253-4193.2015.10.013

Citation:

Chen Xuezhao, Zhang Lei, Yu Shanshan, Bi Caihong, Liu Chunying, Zhu Qian. Molecular cloning and expression analysis of ubiquitin-conjugating enzyme E2-D2 in roughskin sculpin,Trachidermus Fasciatus[J]. Haiyang Xuebao, 2015, 37(10): 133-140. doi: 10.3969/j.issn.0253-4193.2015.10.013

Citation:

Chen Xuezhao, Zhang Lei, Yu Shanshan, Bi Caihong, Liu Chunying, Zhu Qian. Molecular cloning and expression analysis of ubiquitin-conjugating enzyme E2-D2 in roughskin sculpin,Trachidermus Fasciatus[J]. Haiyang Xuebao, 2015, 37(10): 133-140. doi: 10.3969/j.issn.0253-4193.2015.10.013

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Molecular cloning and expression analysis of ubiquitin-conjugating enzyme E2-D2 in roughskin sculpin,Trachidermus Fasciatus

doi: 10.3969/j.issn.0253-4193.2015.10.013

School of Marine Science, Shandong University (Weihai), Weihai 264209, China

Received Date: 2015-03-25

Abstract

Abstract

Ubiquitin-conjugating enzyme is the second enzyme in the process of protein ubiquitination and plays an important role in ubiquitin transferring and substrate specific recognition. In this study,we cloned the full-length cDNA from the Roughskin sculpin,Trachidermus fasciatus by RACE,and named as TfUbcE2-D2. The cDNA of TfUbcE2-D2 is 993 bp,which contained an open reading frame (ORF) of 444 bp encoding a polypeptide of 147 amino acids. The deduced amino acid sequence had a domain called Ubcc,which was extremely conservative. The mRNA expression of TfUbcE2-D2 in healthy and Vibrio anguillarum challenge was detected by quantitative real-time PCR,results showed that TfUbcE2-D2 was expressed in all the detected tissues,mainly expressed in kidney and gill. The up-regulated of TfUbcE2-D2 post challenge with Vibrio anguillarum in blood,spleen,liver and gill indicated that it might be involved in the innate response of Roughskin sculpin.
- ubiquitin-conjugating enzyme,
- Trachidermus fasciatus,
- gene clone,
- innate immunity

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References(22)

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