Analysis on the bacterial abundance and community structure of culturable bacteria in the surface microlayer of the northern Yellow Sea

Fan Jingfeng; Ming Hongxia; Wang Xiaohui; Li Hongbo; Shi Feng; Mu Guiqiang; Zhao Shun

doi:10.3969/j.issn.0253-4193.2015.10.012

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Fan Jingfeng, Ming Hongxia, Wang Xiaohui, Li Hongbo, Shi Feng, Mu Guiqiang, Zhao Shun. Analysis on the bacterial abundance and community structure of culturable bacteria in the surface microlayer of the northern Yellow Sea[J]. Haiyang Xuebao, 2015, 37(10): 123-132. doi: 10.3969/j.issn.0253-4193.2015.10.012

Citation:

Fan Jingfeng, Ming Hongxia, Wang Xiaohui, Li Hongbo, Shi Feng, Mu Guiqiang, Zhao Shun. Analysis on the bacterial abundance and community structure of culturable bacteria in the surface microlayer of the northern Yellow Sea[J]. Haiyang Xuebao, 2015, 37(10): 123-132. doi: 10.3969/j.issn.0253-4193.2015.10.012

Citation:

Fan Jingfeng, Ming Hongxia, Wang Xiaohui, Li Hongbo, Shi Feng, Mu Guiqiang, Zhao Shun. Analysis on the bacterial abundance and community structure of culturable bacteria in the surface microlayer of the northern Yellow Sea[J]. Haiyang Xuebao, 2015, 37(10): 123-132. doi: 10.3969/j.issn.0253-4193.2015.10.012

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Analysis on the bacterial abundance and community structure of culturable bacteria in the surface microlayer of the northern Yellow Sea

doi: 10.3969/j.issn.0253-4193.2015.10.012

1.
National Marine Environmental Monitoring Center, Dalian 116023, China;Key Laboratory of Coastal Ecology and Environment of State Oceanic Administration, Dalian 116023, China
2.
National Marine Environmental Monitoring Center, Dalian 116023, China;Key Laboratory of Coastal Ecology and Environment of State Oceanic Administration, Dalian 116023, China;College of Environmental Science and Engineering, Nankai University, Tianjin 300071

Received Date: 2015-03-23

Abstract

Abstract

In order to learn the bacterial taxa in unique microhabitat of the surface microalyer,the total numbers of bacteria,culturable bacteria and its community structure were investigated from the surface microlayer and subsurface layer of the northern Yellow Sea. Seawater samples were collected from four sampling times,2010-2011. The total number of bacteria was determined by flow cytometry. The number of culturable bacteria was determined by the plate count method. PCR-16S rDNA and sequencing analysis were used to analyze the features of community structure. The results showed that the average numbers of total bacteria are 1.76×10⁶ CFU/mL in the surface microlayer and 1.07×10⁶ cell/mL in subsurface layer,respectively. The number of culturable bacteria ranged from 1.00×10² CFU/mL to 1.70×10⁶ CFU/mL. The percentage that culturable bacteria occupies in total bacteria were 13.05% and 0.45% in the surface microlayer and subsurface layer,respectively. The enrich factor (EF) of total and cultural bacteria were 2.02 and 74.16 in the surface microlayer. The results showed that the culturable bacteria in this region was attached to four phylogenetic communities: Proteobacteria (94.34%)、Firmicutes (1.89%)、Bacteroidetes (1.89%) and Actinobacteria (1.89%). This research preliminary presented that the surface microlayer in seawater has an high enrichment effect on bacteria,especially for culturable bacteria. The bacterial communities in the surface microlayer and subsurface layer was different,which reflected in wide range of species,diversity of source and function. Therefore,the unique bacterial communities of the surface microlayer is generated towing to its special microhabitat. The ecological function of microorganisms in surface microlayer need to be further understood in the future.
- surface microlayer,
- subsurface layer,
- total bacteria,
- culturable bacteria,
- community structure

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