The complete mitochondrial genome of Zhangzhou Coelomactra antiquata：the evidence of a new species in genus Coelomactra (Mollusca：Mactridae)

MENG Xueping; SHEN Xin; ZHAO Nana; TIAN Mei; LIANG Meng; HAO Jue; CHENG Hanliang; YAN Binlun; DONG Zhiguo

doi:10.3969/j.issn.0253-4193.2013.03.024

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MENG Xueping, SHEN Xin, ZHAO Nana, TIAN Mei, LIANG Meng, HAO Jue, CHENG Hanliang, YAN Binlun, DONG Zhiguo. The complete mitochondrial genome of Zhangzhou Coelomactra antiquata：the evidence of a new species in genus Coelomactra (Mollusca：Mactridae)[J]. Haiyang Xuebao, 2013, 35(3): 204-214. doi: 10.3969/j.issn.0253-4193.2013.03.024

Citation:

MENG Xueping, SHEN Xin, ZHAO Nana, TIAN Mei, LIANG Meng, HAO Jue, CHENG Hanliang, YAN Binlun, DONG Zhiguo. The complete mitochondrial genome of Zhangzhou Coelomactra
antiquata：the evidence of a new species in genus Coelomactra (Mollusca：Mactridae)[J]. Haiyang Xuebao, 2013, 35(3): 204-214. doi: 10.3969/j.issn.0253-4193.2013.03.024

Citation:

MENG Xueping, SHEN Xin, ZHAO Nana, TIAN Mei, LIANG Meng, HAO Jue, CHENG Hanliang, YAN Binlun, DONG Zhiguo. The complete mitochondrial genome of Zhangzhou Coelomactra
antiquata：the evidence of a new species in genus Coelomactra (Mollusca：Mactridae)[J]. Haiyang Xuebao, 2013, 35(3): 204-214. doi: 10.3969/j.issn.0253-4193.2013.03.024

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The complete mitochondrial genome of Zhangzhou Coelomactra
antiquata：the evidence of a new species in genus Coelomactra (Mollusca：Mactridae)

doi: 10.3969/j.issn.0253-4193.2013.03.024

1.
Key Laboratory of Marine Biotechnology of Jiangsu province; College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China
2.
Key Laboratory of Marine Biotechnology of Jiangsu province; College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China;Key Laboratory of Marine Biology of Jiangsu province; College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 2012-06-02
Rev Recd Date: 2012-12-30

Abstract

Abstract

Mitochondrial DNA of Zhangzhou Coelomactra antiquata was obtained by long PCR amplification, and sequenced using primers-walking strategy. Then the phylogenetic tree was built with 49 bivalve species to explore the evolutionary status of the Zhangzhou C. antiquata. The results showed that the total length of mitochondrial genome of Zhangzhou C. antiquata is 17 199 bp and A+T content is 64.2%. Thirty-six genes encoding on the same chain were consisted of 12 protein genes, 22 tRNA (one duplication of tRNA^Ser, and two copies of tRNA^Met) genes and two rRNA genes (lrRNA and srRNA). The nucleotide content of non-coding regions is 10.4% (1 842 bp/17 199 bp), one major non-coding region (MNR) is 882 bp, another 400nt non-coding region, which is specific for ZZ C. antiquata, was also found in its mitochondrial genome. Significant differences of MNR between Zhangzhou C. antiquata and Rizhao C. antiquata were observed. As references to three species from Meretrix(Veneroida), four species from Mytilus(Mytiloida), and six species from Crassostrea(Ptenioida), the differences of nucleotides and amino acid sequences of protein coding genes revealed that divergence of Zhangzhou C. antiquata and Rizhao C. antiquata reached the level of interspecies difference. The difference of the encoding genes, tRNA composition and the non-coding regions suggest that Zhangzhou C. antiquata should be a new species of genus Coelomactra.
- Coelomactra antiquata,
- mitochondrial genome,
- genetic difference,
- new species

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References(30)

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