Analysis of expression patterns of key genes in the mTOR signaling pathway in the larval growth and development of <i>Mytilus coruscus</i>

Liang Xinjie; Xu Minhui; Ye yingying; Li jiji

doi:10.12284/hyxb2024088

Volume 46 Issue 7

Jul. 2024

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Liang Xinjie，Xu Minhui，Ye yingying, et al. Analysis of expression patterns of key genes in the mTOR signaling pathway in the larval growth and development of Mytilus coruscus[J]. Haiyang Xuebao，2024, 46(7)：110–119 doi: 10.12284/hyxb2024088

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Liang Xinjie，Xu Minhui，Ye yingying, et al. Analysis of expression patterns of key genes in the mTOR signaling pathway in the larval growth and development of Mytilus coruscus[J]. Haiyang Xuebao，2024, 46(7)：110–119 doi: 10.12284/hyxb2024088

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Analysis of expression patterns of key genes in the mTOR signaling pathway in the larval growth and development of Mytilus coruscus

doi: 10.12284/hyxb2024088

National Engineering Research Center For Marine Aquaculture, Zhejiang Ocean University zhoushan 316022, China

Received Date: 2024-01-28
Rev Recd Date: 2024-04-18

Available Online: 2024-08-13

Publish Date: 2024-07-01

Abstract

Abstract

In the field of aquaculture, a comprehensive exploration of the growth and developmental mechanisms of Mytilus coruscus lays the theoretical foundation for molecular regulation of growth and development. In order to investigate the expression patterns of genes associated with the growth and development differences in M. coruscus larvae, this study employed transcriptome sequencing and real-time quantitative PCR analysis techniques, conducting a preliminary examination of the differential expression of genes and molecular pathways related to growth characteristics. The research places particular emphasis on the impact and regulatory roles of the mammalian target of rapamycin (mTOR) signaling pathway in the growth and development of M. coruscus larvae. By analyzing the gene expression patterns at different developmental stages (Trochophore larvae stage, D-veliger larvae stage, Veliconcha larvae stage, Pediveliger larvae stage, and Juvenile stage), the results suggest that the mTOR signaling pathway may play a specific role in the growth and development of M. coruscus larvae. Seven key genes associated with growth were successfully identified. As the developmental process progresses, the expression of genes in the mTOR signaling pathway exhibits dynamic changes across different developmental stages. Among these changes, the expression levels of the PI3K, TSCI/2, and mTOR genes initially increased, followed by a decrease, and subsequently rose again. In contrast, IGFI expression exhibited an initial increase followed by a decline. Meanwhile, the expression of EIF4B, RPS6KB, and AKT genes demonstrated an overall downward trend. This differential gene expression pattern reflects the potential regulatory influence of the mTOR signaling pathway on cell fate and biological functions during various developmental stages of M. coruscus larvae, thereby impacting their growth and development. Therefore, this study provides a preliminary exploration of the expression patterns of key genes in the mTOR signaling pathway during the growth and development of M. coruscus larvae. These genes play a crucial role in regulating the molecular functions and growth characteristics of M. coruscus larvae, offering foundational data for a deeper understanding of the physiological adaptation, metabolic processes, and growth variations in marine bivalves.
- Mytilus coruscus larvae,
- mTOR signaling pathway,
- gene,
- growth and development

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References

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