Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean

Zhao Sufang; Liu Renju; Dong Chunming; Lv Shiwei; Zhang Benjuan; Shao Zongze

doi:10.12284/hyxb2024066

Volume 46 Issue 5

May 2024

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Zhao Sufang，Liu Renju，Dong Chunming, et al. Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean[J]. Haiyang Xuebao，2024, 46(5)：81–92 doi: 10.12284/hyxb2024066

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Microbial diversity of alkane- and plastic-degrading microbiome in offshore sediments of Ross Sea, Southern Ocean

doi: 10.12284/hyxb2024066

Zhao Sufang^{1, 2
,},
Liu Renju^{1, 3},
Dong Chunming¹,
Lv Shiwei^{1, 3},
Zhang Benjuan¹,
Shao Zongze^{1, 2, 3
,
,}

1.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; Xiamen 361005, China
2.
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, China
3.
School of Environmental Science, Harbin Institute of Technology, Harbin 150090, China

Received Date: 2023-11-11
Rev Recd Date: 2024-03-11

Available Online: 2024-05-15

Publish Date: 2024-05-01

Abstract

Abstract

Oil and plastic pollutants are a serious threat to marine ecosystems and have even been found in the Ross Sea of the Southern Ocean. In order to obtain low-temperature alkane degrading bacteria and plastic-degrading bacteria in the region, a total of twelve sediment samples were collected in the Ross Sea area for enrichment and isolation of alkane-degrading bacteria at low-temperature, and the diversity analyses of the tetradecane-enriched communities showed that the most dominant genera were Pseudomonas, Alcanivorax, Marinomonas, Pseudoalteromonas. The polyethylene terephthalate(PET)and polyethylene(PE)was further validated using the dominant alkane-degrading bacteria. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (ATR-FTIR) demonstrated that the four pure cultures of Pseudomonas pelagia R1-05-CR3, Pseudomonas taeanensis A11-04-CA4, Halomonas titanicae A11-02-7C2 and Rhodococcus cerastii R1-05-7C3 could degrade PE effectively. The results of UPLC-MS and SEM confirmed the PET degradation by isolates of R. cerastii R1-05-7C3, Microbacterium maritypicum RA1-00-CA1, and H. titanicae A11-02-7C2. In conclusion, this study reports the diversity of the tetradecane-enriched consortia at low-temperature and plastic degrading bacteria in the offshore sediments of the Ross Sea in the Southern Ocean, which play a selfpurifying role in in-situ environmental contamination, and also provided strain resources for the biodegradation of hydrocarbon and plastic contaminants at low temperature.
- Ross Sea,
- alkanes,
- polyethylene,
- polyethylene terephthalate,
- biodegradation,
- cold-adapted microorganisms

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References

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