Gene cloning of IGF-2 gene and differential expression of IGF-1/2 during embryonic development in Acanthopagrus latus

Cui Miao; Li Yujie; Yang Yongchun; Zhang Jiaying; Xu Xianji; Lin Liquan; Lin Guorong; Zhang Qizhong; Xu Delin

doi:10.12284/hyxb2022158

Volume 44 Issue 10

Oct. 2022

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Cui Miao，Li Yujie，Yang Yongchun, et al. Gene cloning of IGF-2 gene and differential expression of IGF-1/2 during embryonic development in Acanthopagrus latus[J]. Haiyang Xuebao，2022, 44(10)：152–162 doi: 10.12284/hyxb2022158

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Cui Miao，Li Yujie，Yang Yongchun, et al. Gene cloning of IGF-2 gene and differential expression of IGF-1/2 during embryonic development in Acanthopagrus latus[J]. Haiyang Xuebao，2022, 44(10)：152–162 doi: 10.12284/hyxb2022158

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Gene cloning of IGF-2 gene and differential expression of IGF-1/2 during embryonic development in Acanthopagrus latus

doi: 10.12284/hyxb2022158

1.
College of Life Science and Technology, Jinan University, Guangzhou 510632, China
2.
Yangxi Hengsheng Aquaculture Co., Ltd., Yangjiang 529825, China
3.
Yangjiang Guorong Aquatic Science and Technology Co., Ltd., Yangjiang 529825, China

Received Date: 2022-02-15
Rev Recd Date: 2022-04-04

Available Online: 2022-05-16

Publish Date: 2022-10-01

Abstract

Abstract

Insulin-like growth factors (IGFs) are key regulators downstream of the growth axis and play an important role in promoting cell differentiation and growth. In order to further study the expression mechanism and possible roles of IGF-1 and IGF-2 genes in the embryonic development of Acanthopagrus latus. Our research cloned and identified the cDNA sequence of IGF-2 gene from A. latus and proceeded a biological information analysis. At the same time, based on the foundation of continual observation of the embryonic development of A. latus. RT-qPCR was carried out to explore the expression levels of IGF-1 and IGF-2 genes. The results suggested that the full length and open reading frame of IGF-2 gene are 1 736 bp and 648 bp respectively, encoding a predicted peptide of 215 amino acids. Multiple alignment showed that A. latus IGF-2 was 99.95% similar to Sparus aurata and 98.14% similarity with Pagrus auriga, as well as relatively high homology with other teleosts, indicating that conservation of IGF-2 in teleost evolutionary relationships. Through continuous observation of the embryonic development of A. latus, it was found that under the conditions of water temperature of (26.5±0.5)℃, pH of 8.0 and salinity of 28, the time from fertilization of egg to hatching of membrane of A. latus was 25.5 h. Expression analysis showed that IGF-1 and IGF-2 genes constitutively expresses in various tested stages of embryo of A. latus. The expression of IGF-1 gene showed a trend of first increasing and then decreasing, reaching a relatively high expression in the muscle effect period, and the expression of IGF-2 gene showed a trend of first increasing and then decreasing and then increasing, IGF-2 gene was expressed in the gastrula stage, the muscle effect stage and 3 days after membrane emergence. Summarizing, all the data of our study above showed that IGF-1 and IGF-2 genes may play a crucial role in embryonic development in A. latus.
- insulin-like growth factors,
- gene clone,
- Acanthopagrus latus,
- embryonic development,
- expression pattern

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References

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