Diversity of anaerobic hydrocarbon-degrading bacteria in intertidal sediments and isolation and identification of <i>Desulfovibrio subterraneus</i> ND17

Zhang Qi; Deng Ye; Shao Zongze; Wang Wanpeng

doi:10.12284/hyxb2022154

Volume 44 Issue 8

Aug. 2022

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Zhang Qi，Deng Ye，Shao Zongze, et al. Diversity of anaerobic hydrocarbon-degrading bacteria in intertidal sediments and isolation and identification of Desulfovibrio subterraneus ND17[J]. Haiyang Xuebao，2022, 44(8)：78–86 doi: 10.12284/hyxb2022154

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Zhang Qi，Deng Ye，Shao Zongze, et al. Diversity of anaerobic hydrocarbon-degrading bacteria in intertidal sediments and isolation and identification of Desulfovibrio subterraneus ND17[J]. Haiyang Xuebao，2022, 44(8)：78–86 doi: 10.12284/hyxb2022154

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Diversity of anaerobic hydrocarbon-degrading bacteria in intertidal sediments and isolation and identification of Desulfovibrio subterraneus ND17

doi: 10.12284/hyxb2022154

Zhang Qi^{1, 2
,},
Deng Ye^{1, 3},
Shao Zongze²,
Wang Wanpeng^{2
,
,}

1.
Institute of Marine Science and Technology, Shandong University, Qingdao 266237, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
3.
Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China

Received Date: 2022-02-26
Rev Recd Date: 2022-04-13

Available Online: 2022-04-25

Publish Date: 2022-08-15

Abstract

Abstract

As a junction between land and sea, the intertidal zone is vulnerable to oil pollution from the ocean, and the degradation process of various types of petroleum hydrocarbons entering the sediment is still unclear. There have been many studies on aerobic microbial hydrocarbon degradation, but little has been reported on anaerobic hydrocarbon degradation in the offshore intertidal environment. In this study, anaerobic enrichment culture was conducted on deep intertidal sediment samples from Qingdao Nüdao Bay with mixed hydrocarbons (medium and long chain alkanes and polycyclic aromatic hydrocarbons) as carbon source and sulfate as electron acceptor. The bacterial diversity of the enriched flora indicated that the dominant flora changed to Desulfosarcinaceae, Desulfobacteraceae and other sulfate-reducing bacteria with petroleum hydrocarbon degradation potential under the effect of mixed hydrocarbons as carbon source. An anaerobic hydrocarbon-degrading strain ND17 was obtained by isolation and purification, with 99.93% similarity to the 16S rRNA gene sequence of Desulfovibrio subterraneus HN2^T, the model species of Desulfovibrio subterraneus genus. Further experiments showed that strain ND17 could degrade eicosanoids and phenanthrene up to 53.9% and 35.7%, respectively, under anaerobic conditions. This is the first study on the degradation of petroleum hydrocarbons under anaerobic conditions by a single bacterium of the genus Desulfovibrio. As a widely distributed bacterium in anaerobic environments, this study supports further understanding of its remediation potential in marine petroleum-contaminated environments.
- intertidal zone,
- anaerobic hydrocarbon degradation,
- sulfate-reducing bacteria,
- Desulfovibrio

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References(34)

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