Morphological classification of ichthyoplankton in the Changjiang River Estuary based on DNA barcoding

Liu Shouhai; Yang Yiyi; He Yanlong; Ji Xiao; Wang Yutan; Zhang Haijing; Mao Ruyi; Jiang Xiaoshan; Cheng Xiangsheng

doi:10.12284/hyxb2021062

Volume 43 Issue 8

Aug. 2021

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Liu Shouhai，Yang Yiyi，He Yanlong, et al. Morphological classification of ichthyoplankton in the Changjiang River Estuary based on DNA barcoding[J]. Haiyang Xuebao，2021, 43(8)：93–104 doi: 10.12284/hyxb2021062

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Morphological classification of ichthyoplankton in the Changjiang River Estuary based on DNA barcoding

doi: 10.12284/hyxb2021062

Liu Shouhai^{1, 2
,},
Yang Yiyi^{1, 2},
He Yanlong^{1, 2},
Ji Xiao^{1, 2},
Wang Yutan^{1, 2},
Zhang Haijing³,
Mao Ruyi^{1, 2},
Jiang Xiaoshan^{1, 2},
Cheng Xiangsheng^{1, 2
,
,}

1.
East China Sea Environment Monitoring Center, State Oceanic Administration, Shanghai 201206, China
2.
Key Laboratory of Marine Ecological Monitoring and Restoration Technology, Ministry of Natural Resources, Shanghai 201206, China
3.
East Sea Marine Environmental Investigating and Surveying Center, State Oceanic Administration, Shanghai 200137, China

Received Date: 2020-08-21
Rev Recd Date: 2020-12-25

Available Online: 2021-05-20

Publish Date: 2021-08-25

Abstract

Abstract

DNA barcoding technology is a tool for rapid and accurate species identification using short orthologous DNA sequence. In this study, both traditional morphology and DNA barcoding technology was used to accurately identify ichthyoplankton species in the Changjiang River Estuary and its adjacent waters. Morphological description was carried out at the same time. The results showed that in spring and summer of 2016 and summer of 2017, 55 species of ichthyoplankton were obtained, belonging to 9 orders and 19 families. Among them, there were 35 species of Perciformes at most. The seasonal variation of ichthyoplankton in fish group was not obvious, while there was significant seasonal variation in species. Only Coilia mystus, Engraulis japonius and Larimichthys polyactis appeared both in spring and summer. It was the first time to describe the morphological characteristics of Harpadon nehereus larva and juvenile. In addition, the quantifiable characters, fin development and melanin distribution of larvae and juvenile of Harpadon nehereus, Chelon affinis, Eleutheronema tetradactylum, Sillago japonica and Paraplagusia japonica, were described and drew. The research could provide a scientific basis for the study of fish nursery in estuaries, and also provide a new way for the accumulation of taxonomic data in the early stage of fish development.
- Changjiang River Estuary,
- fish eggs and larvae,
- morphology,
- DNA barcoding

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References(48)

References

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