Effects of <i>Pseudoalteromonas marina</i> flagellin on biofilm formationand settlement of <i>Mytilus coruscus</i>

Cai Yushan; Zhang Xiukun; Zhu Youting; Yang Jinlong; Liang Xiao

doi:10.12284/hyxb2021028

Volume 43 Issue 4

Apr. 2021

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Cai Yushan，Zhang Xiukun，Zhu Youting, et al. Effects of Pseudoalteromonas marina flagellin on biofilm formationand settlement of Mytilus coruscus[J]. Haiyang Xuebao，2021, 43(4)：75–83 doi: 10.12284/hyxb2021028

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Cai Yushan，Zhang Xiukun，Zhu Youting, et al. Effects of Pseudoalteromonas marina flagellin on biofilm formationand settlement of Mytilus coruscus[J]. Haiyang Xuebao，2021, 43(4)：75–83 doi: 10.12284/hyxb2021028

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Effects of Pseudoalteromonas marina flagellin on biofilm formationand settlement of Mytilus coruscus

doi: 10.12284/hyxb2021028

Cai Yushan^{1, 2
,},
Zhang Xiukun^{1, 2},
Zhu Youting^{1, 2, 3},
Yang Jinlong^{1, 2, 3},
Liang Xiao^{1, 2, 3
,
,}

1.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
3.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

Received Date: 2020-07-07
Rev Recd Date: 2020-10-26

Available Online: 2021-06-17

Publish Date: 2021-04-01

Abstract

Abstract

Most marine invertebrates undergo planktonic and benthic stages during growth. As a marine economic shellfish and macrofouling organism, Mytilus coruscus has attracted widespread attention. To explore the interaction between marine bacteria and the mussel settlement, Pseudoalteromonas marina, which has a high inducing activity for the plantigrade settlement of the M. coruscus was chosen and the flagellin was extracted by acid hydrolysis ultracentrifugation. The impact of extracted flagellin mixed with agarose solution to on plantigrade settlement was tested directly. The P. marina biofilms treated with extracted flagellin were used to investigate the inducing capacity on plantigrade settlement. Changes in biomass, bacterial density and extracellular products of the biofilms were analyzed by confocal laser scanning microscopy. The results showed that the mixed gel containing P. marina flagellin and agarose significantly promoted the settlement of plantigrades. In other treatment, the flagellin-treated biofilms also had a high inductivity of plantigrade settlement. Biomass, bacterial density, membrane thickness, extracellular β-polysaccharide, lipid and protein contents all increased. The study reveals that flagellin can directly regulate the settlement of plantigrades and control the inductivity by changing the biological characteristics of the P. marina biofilm indirectly. The study is valuable for clarifying the interaction between bacterial flagellin and plantigrade settlement of the M. coruscus.
- biofilm,
- flagellin,
- Mytilus coruscus,
- plantigrade,
- settlement

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References(23)

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