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| Citation: | Zheng Weibing, Mu Pengfei, Ding Lianshuai, Yang Sisi, Ao Jingqun, Ai Chunxiang, Chen Xinhua. Cloning and expression analysis of large yellow croaker (Larimichthys rocea) IL-10 gene[J]. Haiyang Xuebao, 2017, 39(4): 50-60. doi: 10.3969/j.issn.0253-4193.2017.04.005
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Interleukin-10 (IL-10) is a central anti-inflammatory cytokine that demonstrates immunosuppressive function. In this research, an interleukin-10 (IL-10) homologue has been identified by analyzed the genomic data of large yellow croaker (Larimichthys crocea). Subsequently, the open reading frame (ORF) of cDNA and the genomic DNA sequences have been cloned and analyzed. The genomic DNA of large yellow croaker IL-10(LycIL-10) consisted of 1 869 bp that contains five exons and four introns, sharing the same organization with mammalian IL-10 genes. The open reading frame of LycIL-10 consisted 555 bp that give a predicted 184 amino acid IL-10 molecule. It contains a predicted signal peptide of 22 amino acids in the N-terminal and a mature peptide of 162 amino acids, which contained the vertebrate IL-10 family signature. The LycIL-10 exhibits a conserved IL-10 motif signature and shares 26.49%-77.01% amino acid sequence identity with other known IL-10. Real-time PCR analysis showed that LycIL-10 was constitutively expressed in all tissues tested, especially in spleen and muscle. The LycIL-10 mRNA transcription level could significantly increase in the spleen and head kidney of large yellow croaker after trivalent inactivated vaccines or poly(I:C) stimulated, indicating that LycIL-10 may be involved in suppressing the inflammatory induced by bacterium or virus in the large yellow croaker.
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