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卵形鲳鲹应对流速胁迫的代谢组学分析

张静 戴佳玥 来新昊 刘旭祥 张浩 王学锋 汤保贵

张静,戴佳玥,来新昊,等. 卵形鲳鲹应对流速胁迫的代谢组学分析[J]. 海洋学报,2023,45(5):53–63 doi: 10.12284/hyxb2023060
引用本文: 张静,戴佳玥,来新昊,等. 卵形鲳鲹应对流速胁迫的代谢组学分析[J]. 海洋学报,2023,45(5):53–63 doi: 10.12284/hyxb2023060
Zhang Jing,Dai Jiayue,Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao,2023, 45(5):53–63 doi: 10.12284/hyxb2023060
Citation: Zhang Jing,Dai Jiayue,Lai Xinhao, et al. Metabolomic analysis of Trachinotus ovatus under flow velocity stress[J]. Haiyang Xuebao,2023, 45(5):53–63 doi: 10.12284/hyxb2023060

卵形鲳鲹应对流速胁迫的代谢组学分析

doi: 10.12284/hyxb2023060
基金项目: 国家级大学生创新创业训练计划项目(202210566002);南方海洋科学与工程广东省实验室(湛江)项目(ZJW-2019-06)。
详细信息
    作者简介:

    张静(1977-),辽宁省大连市人,博士,副教授,主要研究方向为渔业生态与环境。E-mail:zjouzj@126.com

    戴佳玥(1998-),江苏省南通市人,主要研究方向为渔业生态与环境。E-mail:daijiayue1998@163.com

    通讯作者:

    汤保贵,博士,副教授,主要研究方向为鱼类养殖与遗传育种。E-mail:zjtbg@163.com

  • 中图分类号: P735;S917.4

Metabolomic analysis of Trachinotus ovatus under flow velocity stress

  • 摘要: 流速是深远海养殖的关键环境因子之一。为了深入解析卵形鲳鲹在流速胁迫下的分子调控机制,分别以静水环境、中等流速(54 cm/s)和高流速(90 cm/s)水流对卵形鲳鲹进行胁迫,利用LC/MS的代谢组学技术探究肝脏内源性代谢物的变化,寻找差异代谢物以及相关的代谢通路。结果表明,与静水组相比,中流速组有51种代谢产物的含量发生显著变化,富集在40条通路中,主要包括半乳糖代谢(galactose metabolism)、糖异生(gluconeogenesis)、不饱和脂肪酸的合成(biosynthesis of unsaturated fatty acids)、花生四烯酸代谢(arachidonic acid metabolism)等;高流速组有40种代谢产物的含量发生显著变化,富集在22条通路中,主要包括饱和脂肪酸的合成(biosynthesis of unsaturated fatty acids)、泛酸和辅酶A的生物合成(pantothenate and CoA biosynthesis)、赖氨酸降解(lysine degradation)、花生四烯酸代谢(arachidonic acid metabolism)、亚油酸代谢(linoleic acid metabolism)等。与中流速组相比,高流速组有31种代谢产物含量发生显著变化,富集在12条通路中,主要包括花生四烯酸代谢(arachidonic acid metabolism)、脂肪细胞因子信号通路(adipocytokine signaling pathway)、半胱氨酸和蛋氨酸代谢(cysteine and methionine metabolism)等。通路分析表明中、高流速胁迫均促进了不饱和脂肪酸代谢、花生四烯酸代谢和泛酸的合成,鱼体通过加强代谢来提高游泳能力,中流速组主要显著影响了半乳糖代谢、半胱氨酸和蛋氨酸的代谢,卵形鲳鲹此时产生了更多能量用于运动;高流速组则显著影响了L-赖氨酸、维甲酸的代谢,揭示了在面对高流速胁迫时,鱼体的游泳能力在下降,部分免疫机能触发。
  • 图  1  QC样品的PCA模型

    a. 正离子模式;b. 负离子模式

    Fig.  1  PCA model of QC samples

    a. Positive ion mode; b. negative ion mode

    图  2  QC样本PCA分析

    a. 正离子模式下A与D;b. 负离子模式A与D;c. 正离子模式下A与F;d 负离子模式A与F;e. 正离子模式下D与F;f. 负离子模式D与F

    Fig.  2  Sample QC PCA analysis

    a. A and D in positive ion mode; b. A and D in negative ion mode; c. A and F in positive ion mode; d. A and F in negative ion mode; .e. D and F in positive ion mode; f. D and F in negative ion mode

    图  3  OPLS-DA得分图

    a. 正离子模式下A与D;b. 负离子模式A与D;c. 正离子模式下A与F;d. 负离子模式A与F;e. 正离子模式下D与F;f. 负离子模式D与F

    Fig.  3  OPLS-DA model

    a. A and D in positive ion mode; b A and D in negative ion mode; c. A and F in positive ion mode; d. A and F in negative ion mode; e. D and F in positive ion mode; f. D and F in negative ion mode

    图  4  差异代谢物数量统计图

    a. 正离子模式;b. 负离子模式

    Fig.  4  Statistical diagram of the number of differential metabolites

    a. Positive ion mode; b. negative ion mode

    图  5  D,F分别与A比较下差异代谢物差异韦恩图

    a. 正离子模式;b. 负离子模式

    Fig.  5  Venn diagram of different metabolites in D and F compared with A, respectively

    a. Positive ion mode; b. negative ion mode

    图  6  3组流速间差异代谢物聚类热图分析

    Fig.  6  Clustered heatmaps of different metabolites among three groups

    图  7  KEGG部分通路显著性气泡图

    Fig.  7  KEGG partial pathway significance Bubble Diagram

    表  1  代谢物鉴定结果

    Tab.  1  Metabolite identification results

    类型共得到的峰的数目二级图谱匹配到
    物质名称
    一级图谱匹配到的
    物质名称
    利用一级图谱和二级图谱
    鉴定到的物质总数目
    无法鉴定的
    物质数目
    POS正离子模式8377361018370
    NEG负离子模式736637997360
    下载: 导出CSV

    表  2  部分关键代谢物

    Tab.  2  Some key metabolites

    组别代谢物名称变化趋势VIPp
    D与A花生四烯酸(Arachidonic acid)10.260.024 9
    棕榈酸(Palmitic acid)3.160.045 8
    泛酸(Pantothenic acid)2.280.049 9
    葡萄糖1-磷酸(Glucose 1-phosphate)1.550.035 1
    5'-S-甲基-5'-硫代腺苷(5'-S-Methyl-5'-thioadenosine)2.930.000 2
    麦芽酚(Maltol)2.130.008 5
    N-乙酰-D-半乳糖胺(N-Acetyl-D-galactosamine)1.080.017 3
    F与A5-氧代-二十碳四烯酸(5-OxoETE)3.310.033 9
    麦芽酚(Maltol)2.410.000 7
    L-赖氨酸(L-Lysine)1.040.005 7
    硬脂酸(Stearic acid)2.170.033 6
    泛酸(Pantothenic acid)2.120.004 6
    二十二碳二烯酸酯(13Z,16Z-Docosadienoic Acid)2.100.008 0
    F与D5-氧代-二十碳四烯酸(5-OxoETE)3.720.016 9
    5'-S-甲基-5'-硫代腺苷(5'-S-Methyl-5'-thioadenosine)2.660.001 1
    维甲酸(Tretinoin)2.080.006 0
    下载: 导出CSV
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  • 收稿日期:  2022-09-03
  • 修回日期:  2022-11-17
  • 网络出版日期:  2022-12-08
  • 刊出日期:  2023-05-01

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