卵形鲳鲹应对流速胁迫的代谢组学分析

张静; 戴佳玥; 来新昊; 刘旭祥; 张浩; 王学锋; 汤保贵

卵形鲳鲹应对流速胁迫的代谢组学分析

张静^{1, 2,},
戴佳玥^1,,
来新昊¹,
刘旭祥¹,
张浩¹,
王学锋^{1, 2},
汤保贵^{1, 2, 3, ,}

1.
广东海洋大学水产学院，广东湛江 524088
2.
南方海洋科学与工程广东省实验室（湛江），广东湛江 524025
3.
广东省水产经济动物病原生物学及流行病学重点实验室，广东湛江 524088

基金项目: 国家级大学生创新创业训练计划项目（202210566002）；南方海洋科学与工程广东省实验室（湛江）项目（ZJW-2019-06）。

详细信息

作者简介:
张静，博士，教授，主要研究方向为渔业生态与环境。E-mail：zjouzj@126.com

戴佳玥，主要研究方向为渔业生态与环境。E-mail：daijiayue1998@163.com

通讯作者:
汤保贵，博士，副教授，主要研究方向为鱼类养殖与遗传育种。E-mail：zjtbg@163.com

中图分类号: S917.4
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出版历程
- 收稿日期: 2022-09-03
- 修回日期: 2022-11-17
- 网络出版日期: 2022-12-08

Metabolomic analysis of Trachinotus ovatus under flow velocity stress

Zhang Jing^{1, 2
,},
Dai Jiayue^1
,,
Lai Xinhao¹,
Liu Xuxiang¹,
Zhang Hao¹,
Wang Xuefeng^{1, 2},
Tang Baogui^{1, 2, 3
, ,}

1.
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China
2.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China
3.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

摘要

摘要: 流速是深远海养殖的关键环境因子之一。为了深入解析卵形鲳鲹在流速胁迫下的分子调控机制，分别以静水环境、中等流速（54 cm/s）和高流速（90 cm/s）水流对卵形鲳鲹进行胁迫，利用LC/MS的代谢组学技术探究肝脏内源性代谢物的变化，寻找差异代谢物以及相关的代谢通路。结果表明，与静水组相比，中流速组有49种代谢产物的含量发生显著变化，富集在40条通路中，主要包括半乳糖代谢（Galactose metabolism）、糖异生（Gluconeogenesis）、不饱和脂肪酸的合成（Biosynthesis of unsaturated fatty acids）、花生四烯酸代谢（Arachidonic acid metabolism）等；高流速组有40种代谢产物的含量发生显著变化，富集在22条通路中，主要包括饱和脂肪酸的合成（Biosynthesis of unsaturated fatty acids）、泛酸和辅酶A的生物合成（Pantothenate and CoA biosynthesis）、赖氨酸降解（Lysine degradation）、花生四烯酸代谢（Arachidonic acid metabolism）、亚油酸代谢（Linoleic acid metabolism）等。与中流速组相比，高流速组有31种代谢产物含量发生显著变化，富集在12条通路中，主要包括花生四烯酸代谢（Arachidonic acid metabolism）、脂肪细胞因子信号通路（Adipocytokine signaling pathway）、半胱氨酸和蛋氨酸代谢（Cysteine and methionine metabolism）等。通路分析表明中、高流速胁迫均促进了不饱和脂肪酸代谢、花生四烯酸代谢和泛酸的合成，鱼体通过加强代谢来提高游泳能力，中流速组主要显著影响了半乳糖代谢、半胱氨酸和蛋氨酸的代谢，卵形鲳鲹此时产生了更多能量用于运动；高流速组则显著影响了L-赖氨酸、维甲酸的代谢，揭示了在面对高流速胁迫时，鱼体的游泳能力在下降，部分免疫机能触发。
- 卵形鲳鲹 /
- 流速胁迫 /
- 代谢组分析
Abstract: Flow velocity is one of the key environmental factors for deep sea aquaculture. In order to further understand the molecular regulation mechanism of golden pompano( T. ovatus) under flow velocity stress, the was stressed under the static water, medium flow velocity (54 cm/s) and high flow velocity (90 cm/s), and the LC/MS metabolomics technology was used to explore the changes of endogenous metabolites in the liver to look for differential metabolites and related metabolic pathways. The results showed that 49 metabolites in the medium flow velocity group had significant difference with which in the static water and were enriched in 40 pathways, mainly including galactose metabolism, gluconeogenesis, biosynthesis of unsaturated fatty acids, arachidonic acid metabolism, etc. 40 metabolites in the high flow velocity group had significant difference with which in the static water and were enriched in 22 pathways, mainly including biosynthesis of unsaturated fatty acids, pantothenate and CoA biosynthesis, lysine degradation, arachidonic acid metabolism, linoleic acid metabolism, etc. Compared with the medium flow velocity group, 31 metabolites in the high flow velocity group had significant difference and were enriched in 12 pathways, mainly including arachidonic acid metabolism, adipocytokine signaling pathway, cysteine and methionine metabolism, etc. Pathway analysis showed that both medium and high flow velocity stress promoted unsaturated fatty acid metabolism, arachidonic acid metabolism and pantothenic acid synthesis. The ability of swimming was improved to against the stress of flow velocity by strengthening metabolism. The medium flow velocity group mainly significantly affected the metabolism of galactose, cysteine and methionine. At this time, more and more energy produced by fish was used for exercise. The high flow velocity group mainly significantly affected the metabolism of L-lysine and retinoic acid, which revealed that the swimming ability of fish might be decreased and some immune functions might be triggered under the high flow velocity stress.
- Trachinotus ovatus /
- velocity stress /
- metabolome analysis

HTML全文

图 1 QC样品的PCA模型：（A）正离子模式；（B）负离子模式

Fig. 1 PCA model of QC samples: （A） positive ion mode; （B） Negative ion mode

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图 2 QC样本PCA分析

A. 正离子模式下A与D；B. 负离子模式A与D；C. 正离子模式下A与F；D. 负离子模式A与F；E. 正离子模式下D与F；F. 负离子模式D与F

Fig. 2 Sample PCA analysis

A. A and D in positive ion mode; B. A and D in negative ion mode; C. A and F in positive ion mode; D. A and F in negative ion mode; .E D and F in positive ion mode; F. D and F in negative ion mode

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图 3 OPLS-DA得分图

A. 正离子模式下A与D；B. 负离子模式A与D；C. 正离子模式下A与F；D. 负离子模式A与F；E. 正离子模式下D与F；F. 负离子模式D与F

Fig. 3 OPLS-DA model

A. A and D in positive ion mode; B. A and D in negative ion mode; C. A and F in positive ion mode; D. A and F in negative ion mode; E. D and F in positive ion mode; F. D and F in negative ion mode

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图 4 差异代谢物数量统计图

A. 正离子模式；B. 负离子模式

Fig. 4 Statistical diagram of the number of differential metabolites

A. Positive ion mode; B. negative ion mode

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图 5 D，F分别与A比较下差异代谢物差异韦恩图

A. 正离子模式；B. 负离子模式

Fig. 5 Venn diagram of different metabolites in D and F compared with A respectively

A. Positive ion mode; B negative ion mode

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图 6 3组流速间差异代谢物聚类热图分析

Fig. 6 Clustered heatmaps of different metabolites among three groups

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图 7 KEGG部分通路显著性气泡图：（A）A与D；（B）A与F；（C）D与F

Fig. 7 KEGG partial pathway significance Bubble Diagram: （A） A and D; （B） A and F; （C） D and F

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表 1 代谢物鉴定结果表

Tab. 1 Metabolite identification results

类型	共得到的peak数目	二级图谱匹配到物质名称	一级图谱匹配到的物质名称	利用一级图谱和二级图谱鉴定到的物质总数目	无法鉴定的物质数目
POS正离子模式	837	736	101	837	0
NEG负离子模式	76	637	99	736	0

下载: 导出CSV

表 2 部分关键代谢物

Tab. 2 Some key metabolites

组别	代谢物名称	变化趋势	VIP	p值
AvsD	花生四烯酸（Arachidonic acid）	↑	10.26	0.024 9
	棕榈酸（Palmitic acid）	↑	3.16	0.045 8
	泛酸（Pantothenic acid）	↑	2.28	0.049 9
	葡萄糖1-磷酸（Glucose 1-phosphate）	↑	1.55	0.035 1
	5'-S-甲基-5'-硫代腺苷（5'-S-Methyl-5'-thioadenosine）	↑	2.93	0.000 2
	麦芽酚（Maltol）	↓	2.13	0.008 5
	N-乙酰-D-半乳糖胺（N-Acetyl-D-galactosamine）	↓	1.08	0.017 3
AvsF	5-氧代-二十碳四烯酸（5-OxoETE）	↑	3.31	0.033 9
	麦芽酚（Maltol）	↓	2.41	0.000 7
	L-赖氨酸（L-Lysine）	↓	1.04	0.005 7
	硬脂酸（Stearic acid）	↑	2.17	0.033 6
	泛酸（Pantothenic acid）	↑	2.12	0.004 6
	二十二碳二烯酸酯（13Z,16Z-Docosadienoic Acid）	↑	2.10	0.008 0
DvsF	5-氧代-二十碳四烯酸（5-OxoETE）	↑	3.72	0.016 9
	5'-S-甲基-5'-硫代腺苷（5'-S-Methyl-5'-thioadenosine）	↓	2.66	0.001 1
	维甲酸（Tretinoin）	↑	2.08	0.006 0

下载: 导出CSV

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