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泥蚶血红蛋白异源四聚体酶解多肽抗菌活性及其机理研究

王娟娟 包永波 王素芳 林志华

王娟娟, 包永波, 王素芳, 林志华. 泥蚶血红蛋白异源四聚体酶解多肽抗菌活性及其机理研究[J]. 海洋学报, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011
引用本文: 王娟娟, 包永波, 王素芳, 林志华. 泥蚶血红蛋白异源四聚体酶解多肽抗菌活性及其机理研究[J]. 海洋学报, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011
Wang Juanjuan, Bao Yongbo, Wang Sufang, Lin Zhihua. Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa[J]. Haiyang Xuebao, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011
Citation: Wang Juanjuan, Bao Yongbo, Wang Sufang, Lin Zhihua. Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa[J]. Haiyang Xuebao, 2015, 37(12): 106-115. doi: 10.3969/j.issn.0253-4193.2015.12.011

泥蚶血红蛋白异源四聚体酶解多肽抗菌活性及其机理研究

doi: 10.3969/j.issn.0253-4193.2015.12.011
基金项目: 国家高技术研究发展计划项目(2012AA10A410);浙江省自然科学重点基金项目(LZ12C190001);浙江省重中之重学科开放基金项目(KF2014002);宁波市自然科学基金(2015A610258)。

Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa

  • 摘要: 采用凝胶层析技术从泥蚶血细胞裂解物中纯化得到两种血红蛋白Tg-HbⅡ(异源四聚体)和Tg-HbⅠ(同源二聚体),回收率分别为48.15%和24.91%。胰蛋白酶酶解Tg-HbⅡ,酶解液经反相C-18色谱柱分离纯化得7种血红蛋白源多肽F1~F7。通过琼脂扩散法检测酶解多肽抑菌活性,结果发现7种多肽对金黄色葡萄球菌、大肠杆菌、恶臭假单胞菌、枯草芽孢杆菌和坚强芽孢杆菌均无抑菌作用,但多肽F2~F7对副溶血弧菌、溶藻弧菌和哈维氏弧菌具有明显抗菌活性,且多肽F2~F7对副溶血弧菌、溶藻弧菌和哈维氏弧菌的MIC值在0.012~0.200 mg/mL范围内。荧光显微镜观察酶解多肽杀菌效果发现多肽杀菌迅速,5 min内弧菌细胞几乎全部死亡。利用透射电子显微镜观察正常的和经抗菌肽处理后的溶藻弧菌和哈维氏弧菌细胞的超微结构,结果显示,对照组正常细胞内部结构完整,而经抗菌肽处理过的细胞其细胞壁完整,但因缺乏细胞内容物支持导致局部区域内陷,细胞质膜明显收缩,细胞内物质外流形成许多空泡从而导致细胞死亡。研究结果表明血红蛋白源抗菌肽对泥蚶致病菌弧菌有明显的抗菌活性,其通过破坏细胞内部结构而起到杀菌作用,这些发现为血红蛋白抗菌机制的研究奠定了基础。
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  • 收稿日期:  2015-07-20
  • 修回日期:  2015-09-28

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