FGF信号途径介导鹿角杯形珊瑚水螅体高温脱离的分子机制探究

王艺; 余秋玉; 曾赛男; 刘兆群; 周智

doi:10.12284/hyxb202500-1

FGF信号途径介导鹿角杯形珊瑚水螅体高温脱离的分子机制探究

doi: 10.12284/hyxb202500-1

王艺^1,,
余秋玉¹,
曾赛男¹,
刘兆群^1, ,,
周智¹

海南大学海洋生物与水产学院，海南海口 570228

基金项目: 国家自然科学基金（42476201），海南大学校科研启动项目（KYQD-23164）。

详细信息

作者简介:
王艺（1994—），女，陕西省铜川市人，硕士研究生，主要从事珊瑚礁耐热分子机理研究。E-mail：wangyi940317@163.com

通讯作者:
刘兆群，博士，教授，主要从事珊瑚礁资源利用研究，E-mail：liuzhaoqun@hainanu.edu.cn

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出版历程
- 收稿日期: 2025-08-29
- 修回日期: 2025-11-19
- 网络出版日期: 2025-12-01

Molecular basis of FGF signaling in heat-induced polyp bailout in Pocillopora damicornis

Wang Yi^1
,,
Yu Qiuyu¹,
Zeng Sainan¹,
Liu Zhaoqun^{1
, ,},
Zhou Zhi¹

School of Marine Biology and Fisheries, Hainan University, Haikou 570228, China

摘要

摘要: 本研究从鹿角杯形珊瑚（Pocillopora damicornis）中鉴定并克隆了2个成纤维细胞生长因子（Fibroblast growth factor，FGF）基因，命名为PdFGF1和PdFGF17。序列分析结果表明，PdFGF1与PdFGF17分别编码195个和149个氨基酸，均含有信号肽和典型的FGF结构域，并具有β–折叠、η–环等拓扑结构。高等动物FGF家族分为FGF1、FGF4、FGF7、FGF8、FGF9、FGF11、FGF19等7个亚家族。多序列比对和系统进化树分析结果表明，PdFGF1属于FGF1亚家族，与人（Homo sapiens）FGF1的序列一致性为30.15%；PdFGF17属于FGF8亚家族，与人FGF1的序列一致性为29.36%。为进一步揭示PdFGF1和PdFGF17的生物学功能，本研究分析了高温诱导鹿角杯形珊瑚水螅体脱离的转录组数据，发现在水螅体高温脱离过程中FGF家族基因（PdFGF1、PdFGF2、PdFGF9、PdFGF17、PdFGF18、PdFGFR1、PdFGFR2、PdFGFR3及PdFGFR4）的表达水平显著上升，且FGF信号途径能够激活下游Ras/MAPK级联反应，驱动细胞骨架重构与细胞黏附调节；Cytoscape基因共表达网络分析还揭示FGF与珊瑚共肉（coenosarc）胞外基质（Extracellular Matrix, ECM）降解相关基因（ADAMTSs、COL21A1、CTSZ、P4HA等）存在显著共表达关系。综上，本研究发现高温激活了鹿角杯形珊瑚的FGF信号途径，促进了共肉组织ECM的降解，进而介导共肉裂解和水螅体脱离。
- 成纤维细胞生长因子 /
- 水螅体脱离 /
- 热胁迫 /
- 加权基因共表达网络分析 /
- 基因克隆
Abstract: In this study, two fibroblast growth factor (FGF) genes, named as PdFGF1 and PdFGF17, were identified and cloned from the coral Pocillopora damicornis. Sequence analysis showed that PdFGF1 and PdFGF17 encode 195 and 149 amino acids, respectively. Both contain a signal peptide and a typical FGF domain, with characteristic β–sheets and η–loops in their topological structures. The FGF family in higher animals can be divided into seven subfamilies (FGF1, FGF4, FGF7, FGF8, FGF9, FGF11, and FGF19). Multiple sequence alignment and phylogenetic analysis revealed that PdFGF1 belongs to the FGF1 subfamily, sharing 30.15% sequence identity with HsFGF1 (Homo sapiens), whereas PdFGF17 clusters with the FGF8 subfamily, sharing 29.36% identity with HsFGF17. To further elucidate their biological roles, transcriptomic analysis was performed on P. damicornis under heat stress induced polyp bailout. The results showed that the expression levels of FGF family genes (PdFGF1, PdFGF2, PdFGF9, PdFGF17, PdFGF18, PdFGFR1, PdFGFR2, PdFGFR3 and PdFGFR4) were significantly upregulated during the detachment process. Moreover, the FGF signaling pathway was found to activate the downstream Ras/MAPK cascade, thereby regulating cytoskeletal remodeling and cell adhesion. Gene co-expression network analysis using Cytoscape further revealed significant co-expression relationships between FGF genes and extracellular matrix (ECM) degrading genes in the coenosarc tissue, including ADAMTSs, COL21A1, CTSZ, and P4HA. Collectively, these findings suggest that heat stress activates the FGF signaling pathway in P. damicornis, promoting ECM degradation and ultimately mediating coenosarc dissociation and polyp bailout.
- Fibroblast growth factor /
- Polyp bailout /
- Thermal stress /
- Weighted gene co-expression network analysis /
- Gene cloning

HTML全文

图 1 FGF家族基因氨基酸序列的系统进化树分析

Fig. 1 Phylogenetic analysis of FGF family gene amino acid sequence

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图 2 PdFGF1结构域和多序列比对

（A）PdFGF1的功能域；（B）PdFGF1的三级结构；（C）PdFGF1多序列比对

Fig. 2 Protein structure domains and multiple sequence alignment of PdFGF1

(A) Functional domains of PdFGF1; (B) Tertiary structure of PdFGF1; (C) Multiple sequence alignment of PdFGF1

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图 3 PdFGF17结构域和多序列比对

（A）PdFGF17的功能域；（B）PdFGF17的三级结构；（C）PdFGF17多序列比对

Fig. 3 Protein structure domains and multiple sequence alignment of PdFGF17

(A) Functional domains of PdFGF17; (B) Tertiary structure of PdFGF17; (C) Multiple sequence alignment of PdFGF17

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图 4 转录组分析及共表达网络构建

（A）S3-S0差异表达基因火山图；（B）WGCNA的软阈值筛选；（C）样本聚类分析；（D）基因聚类树及模块划分

Fig. 4 Transcriptome analysis and co-expression network construction

(A) Volcano plot of S3-S0 DEGs; (B) Soft threshold selection of WGCNA; (C) Sample clustering analysis; (D) Gene clustering tree and module division

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图 5 WGCNA模块筛选及功能富集分析

（A）表型与模块相关性热图；（B）模块基因GO富集分析；（C）表型与模块关联散点图；（D）模块基因KEGG功能分析

Fig. 5 WGCNA module selection and functional enrichment analysis

(A) Heatmap of phenotype and module correlation; (B) GO enrichment analysis of module genes; (C) Scatter plot of phenotype and module association; (D) KEGG functional analysis of module genes

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图 6 FGF基因互作网络图

颜色的深浅代表DMNC计算得分的高低，红色为得分高，黄色为得分低

Fig. 6 Network diagram of FGF genes

Color scale: DMNC score magnitude (red = high, yellow = low)

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表 1 基因克隆引物序列

Tab. 1 Primer sequences of genes used for gene cloning

引物名称	引物序列（5’-3’）	产物大小（bp）
PdEF-CerF	CAGGATGTTTACAAGATTGGAGGT	604
PdEF-CerR	TTGTCACTTTGCCAGCGACTT	604
PdFGF1-F	ATGGATCTATTACAGGCTATTTTTGTC	588
PdFGF1-R	TTAGCTTATTTCCCTGCTTTCAATC	588
PdFGF17-F	ATGAAACACAACACAGCAGTTCTATT	525
PdFGF17-R	TTAAGGAATTGAACTGAGTGAAAAATAC	525
M13-47	CGCCAGGGTTTTCCCAGTCACGAC	/

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表 2 各阶段差异表达基因数量统计

Tab. 2 Statistics of the number of differentially expressed genes at each stage

阶段	差异表达基因总数	显著上调差异表达基因数	显著下调差异表达基因数
S1-S0	20569	1617	1910
S2-S0	20552	4169	6711
S3-S0	20499	4298	7568

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表 3 turquoise共表达模块中Hub基因及其相关性得分

Tab. 3 Hub genes of the turquoise co-expressing module and their correlation score

Rank	Gene name	Score	Rank	Gene name	Score
1	TLR2	0.87849	23	COL21A1	0.83680
2	CAT-4	0.86767	24	HMCN1	0.83430
3	ADAMTS6	0.86348	25	CTRB	0.83372
4	BBOX1	0.86028	26	ARSB	0.83369
5	ALP	0.85866	27	TNIP1	0.83168
6	DAO	0.85319	28	RASA1	0.82733
7	MYOF	0.85051	29	PXDN	0.82697
8	G6PD	0.85011	30	FBN1	0.82631
9	RNF213	0.85010	31	TRAF3	0.82566
10	ADORA1	0.84921	32	FGFR4	0.82450
11	RAPH1	0.84804	33	RAPGEF2	0.81981
12	CTHRC1	0.84599	34	RASGRP3	0.81933
13	TBCE	0.84595	35	CASP3	0.81677
14	DZIP3	0.84586	36	ADAMTS1	0.81438
15	IMSP1	0.84517	37	FGFR2	0.81342
16	ARF	0.84459	38	CA3	0.81249
17	MC4R	0.84417	39	ZnMP-nas14	0.80935
18	ALOX5	0.84146	40	MAP4K3	0.80697
19	MCT10	0.84112	41	P4HA1	0.80662
20	ATF2	0.83929	42	CTSZ	0.80388
21	LRRC74B	0.83893	43	FGF1	0.80222
22	RGS6	0.83718

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