不同饵料培养对罗格尼猛水蚤发育过程的影响

顾靓; 胡思敏; 马林; 张琛; 张贝叶; 黄晖; 刘胜

doi:10.12284/hyxb2024092

不同饵料培养对罗格尼猛水蚤发育过程的影响

doi: 10.12284/hyxb2024092

顾靓^{1, 2,},
胡思敏^{1, 3},
马林⁴,
张琛^{1, 2},
张贝叶^{1, 2},
黄晖^{1, 3, 5},
刘胜^{1, 3, ,}

1.
中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室/广东省应用海洋生物学重点实验室，广东广州 510301
2.
中国科学院大学，北京 100049
3.
三亚海洋科学综合（联合）实验室/海南省热带海洋生物技术重点实验室/三亚海洋生态环境工程研究院，海南三亚 572000
4.
中国科学院海洋研究所海洋生物分类与系统演化实验室，山东青岛 266071
5.
中国科学院海南热带海洋生物实验站，海南三亚海洋生态系统国家野外科学观测研究站，海南三亚 572000

基金项目: 国家自然科学基金（42176118）；国家重点研发计划课题（2022FY100602）。

详细信息

作者简介:
顾靓（1995—），女，河南省南阳市人，硕士，主要从事海洋底栖动物生态学研究。E-mail：guliang21@mails.ucas.ac.cn

通讯作者:
刘胜（1970—），男，四川省广安市人，研究员，主要从事珊瑚礁生态学研究。E-mail：shliu@scsio.ac.cn

中图分类号: Q959.223⁺.32
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出版历程
- 收稿日期: 2024-03-13
- 修回日期: 2024-05-06
- 网络出版日期: 2024-10-16

Effects of different diet culture the developmental process of the Robertgurneya sp.

Gu Liang^{1, 2
,},
Hu Simin^{1, 3},
Ma Lin⁴,
Zhang Chen^{1, 2},
Zhang Beiye^{1, 2},
Huang Hui^{1, 3, 5},
Liu Sheng^{1, 3
, ,}

1.
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
CAS-HKUST Sanya Joint Laboratory of Marine Science Research/Key Laboratory of Tropical Marine Biotechnology of Hainan Province/Sanya Institute of Ocean Eco-Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, SCSIO, Sanya 572000, China
4.
Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
5.
Sanya National Marine Ecosystem Research Station/Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya 572000, China

Funds: National Natural Science Foundation of China (42176118); National Key Research a nd Development Project of C hina (2022FY100602)

More Information

Corresponding author: LIU Sheng, email: shliu@scsio.ac.cn

摘要

摘要: 猛水蚤是分布广泛的小型底栖桡足类，在珊瑚礁区的礁石表生藻席中尤为丰富，由于其生境特殊性，目前对发育过程、培养条件的研究较少。本研究于2023年夏季在海南三亚鹿回头珊瑚礁区采集礁石表生藻席，从中分离出一种猛水蚤，经形态学鉴定其属于罗格尼猛水蚤属（Robertgurneya sp.）。在实验室内对其基本发育过程进行观察和记录，并研究了不同饵料培养方式（单种藻和混合藻）对其发育过程的影响。结果显示，罗格尼猛水蚤的成体体长为0.5～0.7 mm，生命周期为61～68 d。单次怀卵量、产卵次数、繁殖周期和生命周期在不同饵料培养下没有显著差异，但在混合藻培养下，罗格尼猛水蚤在胚胎发育期的平均卵径、孵化率、幼体存活率、无节幼虫Ⅵ期的最大体长明显高于单种藻培养组（p < 0.05），且其胚胎发育阶段和桡足幼体期的累计发育时间明显较短（p < 0.05）。研究结果表明，该猛水蚤具有较短的生命周期和较强的繁殖能力，鉴于藻席中存在丰富的底栖微藻和有机碎屑，栖息其中的猛水蚤可能能够为珊瑚礁小型鱼类等捕食者提供巨大的潜在食物资源，因此在珊瑚礁生态系统的物质循环和能量流通中发挥着重要的作用。
- 猛水蚤 /
- 珊瑚礁 /
- 礁石表生藻席 /
- 饵料 /
- 培养方式 /
- 发育过程
Abstract: Benthic harpacticoid copepods are widely distributed in marine ecosystem, which is particularly abundant in the epilithic algal matrix(EAM) of coral reefs. Due to its unique habitat, there is currently limited research on its developmental process and cultivation methods. In this study, we collected epilithic algal matrix from the coral reef of Luhuitou, Sanya, Hainan in the summer of 2023, from which we isolated one species of Harpacticoida, which was belonging to the genus Robertgurneya based on morphological characteristics. The effects of mono-and mixed-algal cultures on the developmental dynamics of Robertgurneya sp. were observed and recorded. The results showed that the adult body length of the Robertgurneya sp. was 0.5−0.7 mm, with an average life cycle of about 61−68 days. There were no significant differences in egg number per female each time, brood number, reproductive cycle, and life cycle between different feed cultivation techniques. However, under mixed algae culture, the average egg diameter, larval survival rate, and maximum body length of nauplii stage VI in the embryonic development stage of the Robertgurneya sp. were significantly higher than those in the mono-algae culture group (p < 0.05). Moreover, the embryonic development time and cumulative copepodid development time were significantly shorter under mixed-algal culture (p < 0.05). The results indicate that Robertgurneya sp. in EAM have a short life cycle and strong reproductive ability. Considering the abundant benthic microalgae and organic debris in the mats, harpacticoida may be able to provide huge potential food resources for predators such as small fish in coral reefs. Therefore, it may play an important role in the material cycle and energy flow of coral reef ecosystems.
- harpacticoida copepods /
- coral reefs /
- epilithic algal matrix /
- diet /
- cultivation methods /
- developmental process

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图 1 罗格尼猛水蚤的采样区域（红色星号）

Fig. 1 Sample area（red asterisk） of Robertgurneya sp.

下载: 全尺寸图片幻灯片

图 2 罗格尼猛水蚤在胚胎发育期的形态变化

A. 交配受精；B. 多细胞期；C. 壳状胚胎期；D. 附肢原基出现期；E. 色素形成期；F. 眼点出现期；G. 破膜孵化

Fig. 2 Morphological variation during embryonic development of Robertgurneya sp.

A. Ovulation phase; B. multicellular stage; C. formation of carapace type embryo; D. appearance of appendage anlage; E. formation of pigment; F. appearance of eyespot; G. hatching

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图 3 罗格尼猛水蚤在无节幼虫、桡足幼体、成体阶段的形态变化

Fig. 3 Morphological variation during Nauplius、Copepodite and adult of Robertgurneya sp.

下载: 全尺寸图片幻灯片

图 4 不同饵料培养对罗格尼猛水蚤各阶段体长的影响

Fig. 4 Effects of different diet cultivation to the body length of Robertgurneya sp.

下载: 全尺寸图片幻灯片

图 5 不同饵料培养对罗格尼猛水蚤累计发育时间（a），怀卵量（b），孵化率、成活率（c）的影响

Fig. 5 Effects of different diet cultivation to the accumulated developmental time (a), egg number (b), hatching rate and survival rate (c) of Robertgurneya sp.

下载: 全尺寸图片幻灯片

表 1 不同饵料培养对罗格尼猛水蚤繁殖周期、产卵次数、生命周期的影响

Tab. 1 Effects of different diet cultivation on the reproduction cycle,brood number and life cycle of the Robertgurneya sp.

营养类型	繁殖周期/d	产卵次数/只	生命周期/d
单种藻	24.60 ± 1.14^b	6.60 ± 0.55^b	64.6 ± 3.36^b
混合藻	23.80 ± 0.84^b	7.40 ± 0.89^b	65.4 ± 4.51^b
注：相同字母代表不同饵料培养对罗格尼猛水蚤的繁殖周期、产卵次数及生命周期在p < 0.05 水平上无显著差异。

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