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基于环境DNA评价鸭绿江口底栖生态质量状况

王桂营 李宏俊 邵魁双 刘滨玮 楚奇 叶金清

王桂营,李宏俊,邵魁双,等. 基于环境DNA评价鸭绿江口底栖生态质量状况[J]. 海洋学报,2024,46(x):1–14 doi: 10.12284/hyxb2024058
引用本文: 王桂营,李宏俊,邵魁双,等. 基于环境DNA评价鸭绿江口底栖生态质量状况[J]. 海洋学报,2024,46(x):1–14 doi: 10.12284/hyxb2024058
Wang Guiying,Li Hongjun,Shao Kuishuang, et al. Evaluation of benthic ecological quality status in the Yalu River estuary based on environmental DNA[J]. Haiyang Xuebao,2024, 46(x):1–14 doi: 10.12284/hyxb2024058
Citation: Wang Guiying,Li Hongjun,Shao Kuishuang, et al. Evaluation of benthic ecological quality status in the Yalu River estuary based on environmental DNA[J]. Haiyang Xuebao,2024, 46(x):1–14 doi: 10.12284/hyxb2024058

基于环境DNA评价鸭绿江口底栖生态质量状况

doi: 10.12284/hyxb2024058
基金项目: 业务化监测项目(鸭绿江口生态综合监测示范)。
详细信息
    作者简介:

    王桂营(1994—),男,河北省衡水市人,主要从事生物多样性研究。E-mail:2761982646@qq.com

    通讯作者:

    叶金清(1986—),男,辽宁省大连市人,主要从事生物多样性研究。E-mail:jqye@nmemc.orc.cn

  • 中图分类号: X8

Evaluation of benthic ecological quality status in the Yalu River estuary based on environmental DNA

  • 摘要: 研究环境DNA鉴定底栖生物评价生态质量状况的应用潜力,采集17份鸭绿江口底栖生物样品,分别利用环境DNA与形态学鉴定并对所得生态质量评价指数(AMBI、BENTIX、香浓维纳H'、M-AMBI)进行比较分析。结果显示:环境DNA鉴定生物隶属于10纲16目19科20属22种、形态学鉴定生物隶属于9纲27目43科55属57种,共有生物10种;两种鉴定方法得出的AMBI指数间(R = 0.428,p = 0.043,y = 0.32x + 1.08)、BENTIX指数间(R = 0.430,p = 0.043,y = 0.28x + 3.59)存在显著一致性,而香浓维纳H'指数间存在显著差异性;两种鉴定方法得出的AMBI等级间、M-AMBI等级间相似性较高,分别为51.02%、44.90%;两种鉴定方法得出的AMBI与M-AMBI等级更符合实际情况,且评价鸭绿江口整体生态质量状况为良。本研究表明基于环境DNA鉴定底栖生物评价生态质量状况,在海洋环境监测调查中具有较高的应用潜力。
  • 图  1  鸭绿江口采样站位分布

    Fig.  1  Distribution of sampling stations in the Yalu River Estuary

    图  2  OTUs随序列量的稀释曲线

    Fig.  2  Dilution curve of OTUs with sequence size

    图  3  环境DNA鉴定底栖生物相对丰度

    Fig.  3  Environmental DNA identification of the relative abundance of benthic organisms

    图  4  形态学鉴定底栖生物相对丰度

    Fig.  4  Morphological identification of the relative abundance of benthic organism

    图  5  生态质量评价指数

    Fig.  5  Ecological quality assessment index

    图  6  生态质量评价指数拟合图

    Fig.  6  Ecological quality assessment index fit chart

    图  7  生态质量评价指数Pearson相关性热图

    **表示p < 0.01,*表示p < 0.05

    Fig.  7  Heat map of Pearson correlation of ecological quality assessment index

    ** Indicates p < 0.01, * indicates p < 0.05

    图  8  生态质量评价等级

    Fig.  8  Ecological quality assessment level

    图  9  生态质量评价等级百分比

    Fig.  9  Percentage of ecological quality assessment level

    图  10  生态质量评价等级聚类树状图

    a. 全部等级站位聚类;b. 环境DNA等级站位聚类;c. 形态学等级站位聚类;d. 全部等级聚类

    Fig.  10  Clustered dendrogram of ecological quality assessment levels

    a. All rank station clustering, b. environmental DNA rank station clustering, c. morphology rank station clustering, d. all rank clustering

    表  1  生态质量评价指数标准值

    Tab.  1  Standard value of ecological quality evaluation index

    生物指数参考文献
    AMBI指数0~1.2>1.2~3.3>3.3~4.3>4.3~5.5>5.5文献[33]
    BENTIX指数6~4>4~3>3~2.5>2.5~20文献[34]
    H'指数≥44~3≥3~2≥2~1≥<1文献[35]
    M-AMBI指数>0.770.77~0.53>0.53~0.39>0.39~0.2<0.2-
    下载: 导出CSV

    表  2  底栖生物注释率结果统计

    Tab.  2  Statistics of benthic annotation rate results

    站位Y4Y5Y6Y7Y8Y10Y12Y16Y17
    注释率/%1.391.364.732.552.262.393.573.323.93
    站位Y18Y19Y20Y21Y23Y24Y25Y26
    注释率/%3.032.052.373.621.530.952.381.00
    下载: 导出CSV

    A1  鸭绿江口底栖生物名录

    A1  List of benthic organisms at the Yalu River Estuary

    种名拉丁名环境DNA鉴定形态学鉴定
    埃氏蛰龙介Terebella ehrenbergi*
    矮小稚齿虫Prionospio pygmaea*
    博氏双眼钩虾Ampelisca bochi*
    不倒翁虫Sternaspis sculata*
    侧口乳蛰虫Thelepus plagiostoma*
    东方长眼虾Ogyrides orientalis**
    东亚壳菜蛤Musculista senhausia*
    豆形短眼蟹Xenophthalmus pinnotheroides*
    楯鳞鼬虫Heterolepidoderma*
    多丝独毛虫Tharyx multifilis*
    多眼虫Polyophthalmus pictus*
    菲律宾蛤仔Ruditapes philippinarum**
    副栉虫Paramphicteis angustifolia*
    寡节甘吻沙蚕Glycinde gurjanovae**
    广大扁玉螺Natica ampla*
    哈氏美人虾Callianassa harmandi*
    蛤仔属Ruditapes sp.*
    海蜇虾Latreutes anoplonyx*
    海稚虫目Spionida*
    含糊拟刺虫Linopherus ambigua*
    黄海葵Anthopleura xanthogrammia*
    极地蚤钩虾Pontocrates altamarimus*
    棘刺锚参Protankyra bidentata**
    尖叶大狐钩虾Grandifoxus cuspis*
    江户明樱蛤Moerella jedoensis*
    经氏壳蛞蝓Philine kinglipini*
    居虫属Naineris sp.*
    昆士兰稚齿虫Prionospio queenslandica*
    马尔他钩虾属Melita sp.*
    密鳞牡蛎Ostrea denselamellosa*
    囊叶齿吻沙蚕Nephtys caeca*
    拟特须虫Paralacydonia paradoxa*
    鸟蛤目Cardiida*
    鸟嘴尖帽螺Capulus dilatatus*
    纽虫属Lineus sp.*
    欧文虫Owenia fusformis*
    奇异稚齿虫Paraprionospio pinnata*
    巧言虫Eulalia viridis*
    青岛文昌鱼Branchiostoma belcheri**
    日本倍棘蛇尾Amphioplus japonicus*
    日本臭海蛹Travisia japonica*
    日本鼓虾Alpheus japonica*
    日本浪漂水虱Cirolana japonensis*
    日本毛翼虫Mesochaetopterus japonicus*
    日本沙钩虾Byblis japonicus*
    深钩毛虫Sigambra bassi*
    十足目Decapoda*
    双眼钩虾Ampelisca sp.*
    丝鳃虫Cirrutulus tentaculatu*
    丝异须虫Heteromastus filiforms**
    穗鳞虫Halosydnopsis pilosa*
    滩拟猛钩虾Harpiniopsis vadiculus**
    细螯虾Leptochela gracilis*
    线虫属Rhabditophanes sp.*
    相模多精囊海鞘Perophora sagamiensis*
    小头虫Capitella capitata*
    新腹足目Lepetellida*
    烟树蛰虫Pista typha*
    异足索沙蚕Lumbrineris heteropoda*
    鼬虫科Chaetonotidae*
    鼬虫属Chaetonotus sp.*
    原管虫Protula tubularia*
    长吻沙蚕Glycera chirori**
    长锥虫Haploscoloplos elongatus**
    织纹螺Nassarius sp.*
    智利巢沙蚕Diopatra chiliensis*
    中华半突虫Phyllodoce sinensis**
    中华内卷齿蚕Aglaophamus sinensis*
    锥稚虫Aonides oxycephala*
      注:*表示存在该物种。
    下载: 导出CSV

    A2  生态质量评价指数与评价等级

    A2  Ecological quality evaluation index and evaluation level

    站位AMBI1BENTIX1H'1M-AMBI1AMBI2BENTIX2H'2M-AMBI2AMBI1
    等级
    BENTIX1
    等级
    H'1
    等级
    M-AMBI1
    等级
    AMBI2
    等级
    BENTIX2
    等级
    H'2
    等级
    M-AMBI2
    等级
    Y42.982.031.600.773.752.831.520.4224413343
    Y53.002.000.720.442.314.773.470.8724532121
    Y63.002.000.240.572.504.671.590.4324522143
    Y72.982.040.900.673.002.000.390.2624522454
    Y83.012.011.500.712.483.542.850.7124422232
    Y103.852.001.400.592.104.402.720.6534422132
    Y123.502.441.490.570.756.002.860.7734421132
    Y161.144.611.500.921.704.092.890.7611412132
    Y170.225.761.400.911.055.602.320.6511411132
    Y183.002.011.210.662.293.682.540.6724422232
    Y191.495.990.110.441.505.202.320.6021532132
    Y200.025.980.070.570.625.591.910.7111521142
    Y210.155.870.960.741.414.752.250.6211522132
    Y231.654.901.490.682.003.782.730.6621422232
    Y242.952.191.120.550.135.880.890.5524421152
    Y251.575.800.640.721.006.002.250.6321521132
    Y262.672.880.840.531.685.043.280.8923532121
      注:标注1环境DNA鉴定、标注2形态学鉴定
    下载: 导出CSV
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  • 收稿日期:  2023-09-06
  • 修回日期:  2024-01-17
  • 网络出版日期:  2024-03-22

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