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CO2驱动的海水酸化对菲律宾蛤仔组织、免疫和抗氧化酶活及转录水平的影响

林毅 陈强 周思顺 孔鲁闽 黄张帆

林毅,陈强,周思顺,等. CO2驱动的海水酸化对菲律宾蛤仔组织、免疫和抗氧化酶活及转录水平的影响[J]. 海洋学报,2024,46(1):88–100 doi: 10.12284/hyxb2024010
引用本文: 林毅,陈强,周思顺,等. CO2驱动的海水酸化对菲律宾蛤仔组织、免疫和抗氧化酶活及转录水平的影响[J]. 海洋学报,2024,46(1):88–100 doi: 10.12284/hyxb2024010
Lin Yi,Chen Qiang,Zhou Sishun, et al. Effects of CO2-driven seawater acidification on tissue, immune and antioxidant enzyme activity and transcription levels of Ruditapes philippinarum[J]. Haiyang Xuebao,2024, 46(1):88–100 doi: 10.12284/hyxb2024010
Citation: Lin Yi,Chen Qiang,Zhou Sishun, et al. Effects of CO2-driven seawater acidification on tissue, immune and antioxidant enzyme activity and transcription levels of Ruditapes philippinarum[J]. Haiyang Xuebao,2024, 46(1):88–100 doi: 10.12284/hyxb2024010

CO2驱动的海水酸化对菲律宾蛤仔组织、免疫和抗氧化酶活及转录水平的影响

doi: 10.12284/hyxb2024010
基金项目: 福建省自然科学基金项目(2020J01668)。
详细信息
    作者简介:

    林毅(1999—),男,福建省永安市人,主要从事贝类养殖研究。E-mail:202111908039@jmu.edu.cn

    通讯作者:

    陈强,博士,硕士生导师,主要从事生态环境修复研究。E-mail: ahcq@jmu.edu.cn

  • 中图分类号: P714+.5;S944.4+7

Effects of CO2-driven seawater acidification on tissue, immune and antioxidant enzyme activity and transcription levels of Ruditapes philippinarum

  • 摘要: 随着CO2的大量排放,海洋酸化效应不断加重,为探究未来海水酸化情况对菲律宾蛤仔产生的影响,设置对照组(pH为8.1)和酸化组(pH为7.7、7.1和6.4),研究周期为42 d,测定菲律宾蛤仔在酸化条件下组织结构、免疫和抗氧化酶活性的变化情况,以及在分子水平上产生的影响。结果表明:菲律宾蛤仔置于酸化海水环境中,鳃丝间距随pH的降低而扩大,鳃丝纤毛黏合,水管和外套膜外表皮褶皱逐渐加深;鳃组织中酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)活性变化情况为先降后升,碱性磷酸酶(AKP)活性各组变化趋势不同,总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和溶菌酶(LZM)活性趋势为先升后降;鳃和内脏团谷胱甘肽过氧化物酶(GSH-Px)活性变化规律皆为持续上升;内脏团组织中LZM活性变化趋势各不相同,ACP活性变动趋势为先降后升,AKP、SOD和CAT活性变化规律为先升后降,T-AOC趋势为持续下降;通过转录组的分析得到,鳃组织GO功能主要富集在DNA整合、膜的组成部分和RNA定向DNA聚合酶活性等条目中,KEGG通路主要富集在吞噬体和与蛋白合成的相关通路中。海水酸化使菲律宾蛤仔组织呈现不同程度的损伤,破坏其内环境稳态,改变其代谢水平和与免疫相关基因表达,引发菲律宾蛤仔染病乃至死亡的风险上升。
  • 图  1  海水酸化对菲律宾蛤仔不同组织结构的影响

    A. DD组鳃;B. CC组鳃;C. BB组鳃;D. AA组鳃;E. DD组水管;F. CC组水管;G. BB组水管;H. AA组水管;I. DD组外套膜;J. CC组外套膜;K. BB组外套膜;L. AA组外套膜

    Fig.  1  Effects of seawater acidification on different organization structure of Ruditapes philippinarum

    A. Group DD gill; B. Group CC gill; C. Group BB gill; D. Group AA gill; E. Group DD pipe; F. Group CC pipe; G. Group BB pipe; H. Group AA pipe; I. Group DD mantle; J. Group CC mantle; K. Group BB mantle; L. Group AA mantle

    图  2  海水酸化对鳃组织免疫酶活性的影响

    上标不同小写字母表示各组之间差异显著(p < 0.05)

    Fig.  2  The immunoenzyme activity of gill stressed by ocean acidification

    Different superscript lowercase letters indicate significant differences between groups (p < 0.05)

    图  3  海水酸化对内脏团免疫酶活性的影响

    上标不同小写字母表示各组之间差异显著(p < 0.05)

    Fig.  3  The immunoenzyme activity of visceral mass stressed by ocean acidification

    Different superscript lowercase letters indicate significant differences between groups (p < 0.05)

    图  4  海水酸化对鳃组织抗氧化酶活性的影响

    上标不同小写字母表示各组之间差异显著(p < 0.05)

    Fig.  4  The antioxidant enzyme activity of gill stressed by ocean acidification

    Different superscript lowercase letters indicate significant differences between groups (p < 0.05)

    图  5  海水酸化对内脏团抗氧化酶活性的影响

    上标不同小写字母表示各组之间差异显著(p < 0.05)

    Fig.  5  The antioxidant enzyme activity of visceral mass stressed by ocean acidification

    Different superscript lowercase letters indicate significant differences between groups (p < 0.05)

    图  6  差异表达基因数目统计

    Fig.  6  Statistics of the number of differentially expressed genes

    图  7  差异基因qRCR和RNA-seq验证

    Fig.  7  The qRCR and RNA-seq validation of differentially expressed genes

    表  1  菲律宾蛤仔鳃组织文库测序数据统计分析

    Tab.  1  Statistical analysis of sequencing data of gill library of Ruditapes philippinarum

    样品序列数质控数据总碱基数/bpGC含量/%Q20/%Q30/%
    AA119 805 2565 921 771 25236.8197.4292.53
    AA221 015 8646 280 860 79636.9497.5492.78
    AA319 731 7745 901 090 29837.1897.592.68
    BB120 674 9086 180 447 03637.4597.7693.27
    BB220 510 3826 131 925 98837.496.5390.69
    BB320 992 1986 275 544 49835.8197.1191.92
    CC121 890 3146 542 576 53236.4497.1191.92
    CC226 002 3717 775 975 50236.7997.5592.78
    CC319 225 6505 747 607 41836.9297.7693.31
    DD126 236 7657 799 003 78037.597.6693.08
    DD221 474 7856 390 329 89236.497.5292.79
    DD320 597 5746 154 866 69636.7497.2792.24
    下载: 导出CSV

    表  2  差异表达基因GO功能富集

    Tab.  2  GO functional enrichment of differentially expressed genes

    差异表达基因类型 GO条目 p
    AA vs. DD BB vs. DD CC vs. DD AA vs. BB AA vs. CC BB vs. CC
    上调基因 DNA整合 1.48 × 10−5 1.61 × 10−10 1.84 × 10−4 3.79 × 10−5 5.59 × 10−5 5.03 × 10−9
    膜的组成部分 1.85 × 10−2 5.85 × 10−4 0.43 1.13 × 10−2 5.07 × 10−4 1.04 × 10−2
    核糖体 5.99 × 10−5 0.92 1.37 × 10−2 1.49 × 10−2 1.54 × 10−4 0.14
    RNA定向DNA聚合酶活性 0.59 1.24 × 10−2 1.17 × 10−4 1.93 × 10−5 7.16 × 10−2 1.52 × 10−4
    下调基因 包涵体组装的负调节 4.74 × 10−5 0.70 0.34 4.79 × 10−5 3.45 × 10−4 0.68
    蛋白水解 5.32 × 10−5 3.70 × 10−7 6.20 × 10−6 2.48 × 10−2 5.14 × 10−2 9.56 × 10−2
    细胞外区域 1.61 × 10−11 8.53 × 10−12 1.83 × 10−4 2.79 × 10−12 4.18 × 10−14 1.55 × 10−9
    细胞外空间 1.56 × 10−8 1.57 × 10−5 2.52 × 10−2 5.41 × 10−7 1.36 × 10−5 2.79 × 10−4
    内肽酶抑制剂活性 2.06 × 10−7 7.27 × 10−8 1.16 × 10−7 6.57 × 10−2 0.29 2.26 × 10−3
    下载: 导出CSV

    表  3  差异表达基因KEGG信号通路富集

    Tab.  3  KEGG pathways enriched for differentially expressed genes

    差异表达基因类型 通路名称 基因数目
    AA vs. DD BB vs. DD CC vs. DD AA vs. BB AA vs. CC BB vs. CC
    上调基因 真核生物中的核糖体生物发生 41 6 1 33 45 6
    核糖体 85 25 42 57 76 33
    Notch信号传导途径通路 18 21 15 17 15 15
    NOD-like受体信号通路 13 16 13 17 19 15
    泛素介导的蛋白水解 32 21 20 44 43 26
    下调基因 吞噬体 41 25 20 42 35 20
    内质网中的蛋白加工 12 7 8 21 14 2
    细胞外基质受体作用 17 16 10 23 16 8
    C-型凝集素受体信号通路 14 7 9 13 19 14
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-05-20
  • 修回日期:  2023-10-09
  • 网络出版日期:  2023-11-30
  • 刊出日期:  2024-01-01

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