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基于环境DNA技术的西沙礁区长棘海星种群丰度研究

闫智聪 邢家杰 蔡文启 张开典 吴钟解 李元超 唐佳 周智

闫智聪,邢家杰,蔡文启,等. 基于环境DNA技术的西沙礁区长棘海星种群丰度研究[J]. 海洋学报,2023,45(3):76–83 doi: 10.12284/hyxb2023038
引用本文: 闫智聪,邢家杰,蔡文启,等. 基于环境DNA技术的西沙礁区长棘海星种群丰度研究[J]. 海洋学报,2023,45(3):76–83 doi: 10.12284/hyxb2023038
Yan Zhicong,Xing Jiajie,Cai Wenqi, et al. Study on the population distribution of Acanthaster planci in the reef area of the Xisha Islands based on environmental DNA technology[J]. Haiyang Xuebao,2023, 45(3):76–83 doi: 10.12284/hyxb2023038
Citation: Yan Zhicong,Xing Jiajie,Cai Wenqi, et al. Study on the population distribution of Acanthaster planci in the reef area of the Xisha Islands based on environmental DNA technology[J]. Haiyang Xuebao,2023, 45(3):76–83 doi: 10.12284/hyxb2023038

基于环境DNA技术的西沙礁区长棘海星种群丰度研究

doi: 10.12284/hyxb2023038
基金项目: 海南省自然科学基金(420CXTD432,2019RC067);国家自然科学基金(42076145)。
详细信息
    作者简介:

    闫智聪(1999-),男,山西省运城市人,主要从事珊瑚礁保护研究。E-mail:3248201687@qq.com

    通讯作者:

    吴钟解(1981-),男,副研究员,硕士生导师,主要从事珊瑚礁生态学研究。E-mail:289663247@qq.com

    周智(1983-),男,研究员,博士生导师,主要从事珊瑚礁生物学研究。E-mail:zhouzhi@hainanu.edu.cn

  • 中图分类号: Q178.53;Q523

Study on the population distribution of Acanthaster planci in the reef area of the Xisha Islands based on environmental DNA technology

  • 摘要: 长棘海星(Acanthaster planci)作为珊瑚的天敌之一,因其对珊瑚礁生态系统的灾难性破坏而备受关注。然而,长棘海星在南海珊瑚礁生态系统中的时空分布特征仍不清楚。本研究于2020年9月、2021年4月和2022年1月对西沙群岛礁区表层海水进行取样,借助环境DNA(environmental DNA)和实时荧光定量PCR技术分析了表层海水中长棘海星线粒体细胞色素-c-氧化酶亚基I(COTS-mtCOI)基因片段浓度的时空变化,及其与海水温度、盐度、pH、叶绿素含量和营养盐含量等环境因子的相关性。结果发现,2020−2022年,西沙礁区COTS-mtCOI片段浓度的变化范围为0~4.13×107 拷贝数/m3,且永乐环礁附近一直有较高的COTS-mtCOI片段浓度。对于华光礁、晋卿岛、羚羊礁、全富岛和赵述岛而言,2020年9月表层海水中COTS-mtCOI片段的平均浓度显著高于2021年4月和2022年1月(p<0.05)。此外,COTS-mtCOI片段浓度与表层海水的温度显著正相关(p<0.05)。研究结果表明,当前长棘海星群体广泛分布于我国西沙群岛海域,永乐环礁可能分布着较高密度的长棘海星群体,水温升高可能促进长棘海星的暴发。本研究有助于了解南海珊瑚礁生态系统中长棘海星的种群分布特征,同时也能够对长棘海星暴发的预警预报提供理论依据。
  • 图  1  西沙群岛采样站点位置

    Fig.  1  Location of the sampling stations in the Xisha Islands

    图  2  2020年9月西沙群岛表层海水中COTS-mtCOI片段浓度的空间变化

    Fig.  2  Spatial changes of COTS-mtCOI fragment concentration in the surface seawater of the Xisha Islands in September 2020

    图  3  2021年4月西沙群岛表层海水中COTS-mtCOI片段浓度的空间变化

    Fig.  3  Spatial changes of COTS-mtCOI fragment concentration in the surface seawater of the Xisha Islands in April 2021

    图  4  2022年1月西沙群岛表层海水中COTS-mtCOI片段浓度的空间变化

    Fig.  4  Spatial changes of COTS-mtCOI fragment concentration in the surface seawater of the Xisha Islands in January 2022

    图  5  华光礁、晋卿岛、羚羊礁、全富岛和赵述岛表层海水中COTS-mtCOI片段浓度的变化

    a. 5个礁区COTS-mtCOI片段平均浓度随时间的变化;b. 各个礁区COTS-mtCOI片段浓度随时间的变化;*和不同字母分别表示不同日期和相同站点不同日期COTS-mtCOI片段浓度存在显著差异(p<0.05)

    Fig.  5  Changes of COTS-mtCOI fragment concentration in the surface seawater of Huaguang Reef, Jinqing Island, Lingyang Reef, Quanfu Island and Zhaoshu Island reef areas

    a. Mean concentration changes of COTS-mtCOI fragment in the five reef areas over time; b. concentration changes of COTS-mtCOI fragment of individual reef areas over time; * and different letters indicate COTS-mtCOI fragment concentration in the same date and in the different date of the same sampling stations significant differences (p<0.05)

    图  6  西沙群岛各礁区表层海水水质指标和COTS-mtCOI片段浓度的相关性

    *表示存在显著正相关(p<0.05)

    Fig.  6  Correlation between water quality parameters of the surface seawater and COTS-mtCOI fragment concentration of Xisha Islands reef area

    * Indicates a significant positive correlation (p<0.05)

    表  1  各海水水质指标的检测方法

    Tab.  1  Method for analysis of seawater quality indexes

    水质指标检测方法
    温度表层水温表法
    pHpH计法
    盐度盐度计法
    氨氮含量靛酚蓝分光光度法
    亚硝酸盐氮含量盐酸萘乙二胺分光光度法
    硝酸盐氮含量锌镉还原法
    活性磷酸盐含量磷钼蓝分光光度法
    无机磷含量过硫酸钾氧化法
    石油含量分析紫外分光光度法
    叶绿素含量荧光光度法
    悬浮物含量重量法
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-04-10
  • 修回日期:  2022-10-12
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2023-02-01

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