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熊本牡蛎、葡萄牙牡蛎和长牡蛎组织菌群构成及功能的比较分析

叶静 戴文芳 刘圣 林志华 薛清刚

叶静,戴文芳,刘圣,等. 熊本牡蛎、葡萄牙牡蛎和长牡蛎组织菌群构成及功能的比较分析[J]. 海洋学报,2022,44(9):1–12 doi: 10.12284/hyxb2022144
引用本文: 叶静,戴文芳,刘圣,等. 熊本牡蛎、葡萄牙牡蛎和长牡蛎组织菌群构成及功能的比较分析[J]. 海洋学报,2022,44(9):1–12 doi: 10.12284/hyxb2022144
Ye Jing,Dai Wenfang,Liu Sheng, et al. Comparison of the composition and functional potentials of bacterial communities in different tissues from Crassostrea sikamea, Crassostrea angulata and Crassostrea gigas[J]. Haiyang Xuebao,2022, 44(9):1–12 doi: 10.12284/hyxb2022144
Citation: Ye Jing,Dai Wenfang,Liu Sheng, et al. Comparison of the composition and functional potentials of bacterial communities in different tissues from Crassostrea sikamea, Crassostrea angulata and Crassostrea gigas[J]. Haiyang Xuebao,2022, 44(9):1–12 doi: 10.12284/hyxb2022144

熊本牡蛎、葡萄牙牡蛎和长牡蛎组织菌群构成及功能的比较分析

doi: 10.12284/hyxb2022144
基金项目: 国家自然科学基金(32073010,31672629);宁波市“泛3315计划”现代农业创新团队项目(2019-2023)。
详细信息
    作者简介:

    叶静(1995—),女,广西壮族自治区钦州市人,主要从事海洋贝类微生物生态学研究。E-mail: yjing_7758@163.com

    通讯作者:

    薛清刚,教授,主要从事海水养殖动物疾病及其防控研究。E-mail: qxue@zwu.edu.cn

  • 中图分类号: S917.1

Comparison of the composition and functional potentials of bacterial communities in different tissues from Crassostrea sikamea, Crassostrea angulata and Crassostrea gigas

  • 摘要: 为探究健康二龄熊本牡蛎、葡萄牙牡蛎和长牡蛎5种组织间细菌群落组成、多样性和功能的差异,本研究利用Illumina高通量测序技术和PICRUSt2对3种牡蛎5种组织中的细菌群落构成及其潜在功能进行了比较分析。结果显示,在3种牡蛎的5种组织中共鉴定到6 220个细菌OTUs,其中3种牡蛎整体间共有的OTUs数占42.4%。3种牡蛎5种组织中的优势菌主要隶属于γ-变形菌纲(γ-proteobacteria)、α-变形菌纲(α-proteobacteria)、柔壁菌门(Tenericutes)和厚壁菌门(Firmicutes)。弧菌属(Vibrio)在熊本牡蛎的鳃、外套膜、性腺和血淋巴中的相对丰度均显著高于长牡蛎和葡萄牙牡蛎,且分别在熊本牡蛎外套膜、长牡蛎性腺和葡萄牙牡蛎血淋巴中相对丰度最高,但均在3种牡蛎肝胰腺中相对丰度最低。3种牡蛎5种组织间的菌群多样性均有所差异,其中在熊本牡蛎鳃、葡萄牙牡蛎外套膜和长牡蛎血淋巴中菌群多样性最高,而在熊本牡蛎血淋巴、葡萄牙牡蛎性腺和长牡蛎肝胰腺中菌群多样性最低。在不区分组织的情况下,3种牡蛎整体间的菌群结构存在显著差异(r=0.661,p<0.001)。此外,在熊本牡蛎肝胰腺、葡萄牙牡蛎鳃和长牡蛎血淋巴中细菌参与的能量代谢相关功能通路的相关丰度显著高于对应牡蛎的其他组织。本文结果表明,熊本牡蛎、葡萄牙牡蛎和长牡蛎组织内的菌群构成存在差异,且由细菌介导的功能也随牡蛎种类和组织类型发生改变。
  • 图  1  本研究采集的长牡蛎、葡萄牙牡蛎和熊本牡蛎样品图片

    Fig.  1  The pictures of Crassostrea gigas, Crassostrea angulata and Crassostrea sikamea collected in this study

    图  2  维恩图展示3种牡蛎相同组织间(a)和同种牡蛎不同组织间(b)共有和特有的细菌OTU数

    Fig.  2  Venn diagram displaying the numbers of unique and shared OTU among same tissues from three oysters (a) and different tissues (b) from individual oyster

    图  3  3种牡蛎5种组织中的优势细菌门(变形菌门归类到纲)(a)和细菌属(b)的平均相对丰度

    Cs. 熊本牡蛎;Ca. 葡萄牙牡蛎;Cg. 长牡蛎

    Fig.  3  Average relative abundances of the dominant bacterial phyla (Proteobacteria were assigned to the class level) (a) and genus (b) in the five tissues form three oysters

    Cs. Crassostrea sikamea; Ca. Crassostrea angulate; Cg. Crassostrea gigas

    图  4  比较3种牡蛎5种组织中的细菌群落α-多样性差异

    Cs. 熊本牡蛎;Ca. 葡萄牙牡蛎;Cg. 长牡蛎;采用单因素方差分析3种牡蛎种类间在相同组织情况下细菌群落α-多样性的差异,不同字母表示3种牡蛎种类具有差异性( p<0.05)

    Fig.  4  Comparison of bacterial community α-diversity in five tissues from three oysters

    Cs. Crassostrea sikamea; Ca. Crassostrea angulate; Cg. Crassostrea gigas; the difference of bacterial community α-diversity among three oysters under the same tissue was analyzed by one-way variance analysis, of which showed differences using the different letters (p<0.05)the difference of bacterial community α-diversity among three oysters under the same tissue was analyzed by one-way variance analysis, of which showed differences using the different letters (p<0.05)

    图  5  非度量多维尺度分析(NMDS)展示3种牡蛎5种组织间(a)、3种牡蛎整体间(b)和同种牡蛎5种组织间(c)的细菌群落结构差异

    Fig.  5  Non-metric multidimensional scaling analysis (NMDS) showed the difference in bacterial community structures among five tissues (a) and all tissues of three oysters (b) and five tissues of same oyster (c)

    图  6  热图展示3种牡蛎的肝胰腺、鳃、血淋巴中细菌群落功能途径的丰度(平方根转换)分布

    Cs. 熊本牡蛎;Ca. 葡萄牙牡蛎;Cg. 长牡蛎

    Fig.  6  Heatmap showing the abundance (sqrt(x) transformed) distribution of functional pathways of bacterial communities in the gill, hepatopancreas, and hemolymph from three oyster

    Cs. Crassostrea sikamea; Ca. Crassostrea angulate; Cg. Crassostrea gigas

    表  1  基于Bray-Curtis距离比较3种牡蛎不同组织间细菌群落的整体差异性

    Tab.  1  Comparison of overall differences in bacterial communities among different tissues of the three oysters based on Bray-Curtis distance

    组别所有组织肝胰腺外套膜性腺血淋巴
    RpRpRpRpRpRp
    3种牡蛎0.6610.0010.7210.0010.5800.0010.9790.0010.9150.0010.4780.001
    Cs vs Ca0.6330.0011.0000.0030.2830.0621.0000.0030.9980.0030.4370.014
    Cs vs Cg0.4860.0010.7240.0030.4350.0031.0000.0030.9090.0030.5430.009
    Ca vs Cg0.5320.0010.7690.0030.8850.0030.8000.0030.8740.0030.5720.003
    注:加粗的数值表示差异显著(p<0.05)。
    下载: 导出CSV

    表  2  基于 Bray-Curtis 距离比较同种牡蛎不同组织间细菌群落的差异性

    Tab.  2  Comparison of difference in bacterial communities among different tissues from the same oyster based on Bray-Curtis distance

    牡蛎种类组织类型外套膜性腺肝胰腺血淋巴
    熊本牡蛎0.2470.4650.4710.013
    外套膜0.0140.4260.4410.148
    性腺0.0030.0110.5010.38
    肝胰腺0.0030.0020.0020.462
    血淋巴0.3120.0710.0020.011
    葡萄牙牡蛎 0.4820.0570.3570.283
    外套膜0.0040.1940.2440.445
    性腺0.2200.0280.2170.383
    肝胰腺0.0250.0300.0340.4
    血淋巴0.0050.0050.0040.010
    长牡蛎 0.4130.1070.9480.53
    外套膜0.0020.5071.0000.625
    性腺0.0960.0050.9670.369
    肝胰腺0.0030.0020.0020.957
    血淋巴0.0020.0020.0070.003
    注:每个种类的上三角和下三角分别为样本间细菌群落差异性的R值和p值;加粗的数值表示差异显著(p<0.05)。
    下载: 导出CSV

    表  3  基于非参数的多元置换方差分析牡蛎种类和组织类型对细菌群落的定量影响

    Tab.  3  Quantitative effects of oyster species and tissue types on the variations in bacterial community based on based on nonparametric permutational multivariate analysis of variance (PERMANOVA)

    自由度平方和均方差F模型R2p
    牡蛎种类26.8883.44420.2830.2580.001
    组织类型127.1170.5933.4930.2660.001
    残差7512.7350.1700.476
    总计8926.7411
    注:加粗的数值表示差异显著(p<0.05);−代表无数值。
    下载: 导出CSV
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  • 收稿日期:  2021-12-24
  • 修回日期:  2022-03-22

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