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瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析

吕庭莉 杲绍强 张虎 张易婵 陈鹏 王嘉伟 乔帼 杨伯平 张明明

吕庭莉,杲绍强,张虎,等. 瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析[J]. 海洋学报,2022,44(2):84–93 doi: 10.12284/hyxb2022066
引用本文: 吕庭莉,杲绍强,张虎,等. 瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析[J]. 海洋学报,2022,44(2):84–93 doi: 10.12284/hyxb2022066
Lü Tingli,Gao Shaoqiang,Zhang Hu, et al. Study on cell classificatioin, immune-related enzymatic characteristics and preliminary transcriptome of coelomocytes in Onchidium struma[J]. Haiyang Xuebao,2022, 44(2):84–93 doi: 10.12284/hyxb2022066
Citation: Lü Tingli,Gao Shaoqiang,Zhang Hu, et al. Study on cell classificatioin, immune-related enzymatic characteristics and preliminary transcriptome of coelomocytes in Onchidium struma[J]. Haiyang Xuebao,2022, 44(2):84–93 doi: 10.12284/hyxb2022066

瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析

doi: 10.12284/hyxb2022066
基金项目: 国家自然科学基金面上项目(32071615);江苏省水生生物资源重大专项(ZYHB16-5)。
详细信息
    作者简介:

    吕庭莉(1996-),女,江苏省徐州市人,研究方向为水产养殖。E-mail:1207972508@qq.com

    通讯作者:

    张明明,男,博士,副教授,研究方向为生态学和物种资源保护。E-mail:zhangmingm44ok@163.com

  • 中图分类号: S917.4

Study on cell classificatioin, immune-related enzymatic characteristics and preliminary transcriptome of coelomocytes in Onchidium struma

  • 摘要: 瘤背石磺(Onchidium struma)广泛分布于潮间带和潮下带,是海洋生物向陆地生物辐射进化的重要代表,也是环境监测的指示生物。为丰富瘤背石磺的基础免疫学资料,本文研究了瘤背石磺体腔细胞的类别、细胞密度和体腔液免疫相关酶活性,并通过RNA-Seq测序获得体腔细胞转录本,进行生物信息学分析。结果表明,瘤背石磺主要有变形细胞、球形细胞和色素细胞3种不同类型的体腔细胞,其中,球形细胞数量最多,色素细胞数量最少;在无任何外源刺激下,体腔液内酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶和酚氧化酶等抗氧化酶和免疫相关酶均具有活性,且超氧化物歧化酶和酚氧化酶活性最高;转录组学分析显示,体腔细胞中共组装出63 097个Unigenes,KEGG将这些Unigenes主要注释到“信号转导” “运输和分解代谢” “内分泌系统” “翻译” “折叠、分选和降解”等信号通路。研究结果将利于进一步揭示瘤背石磺的体腔液及其体腔细胞在机体免疫防御过程中的作用,丰富瘤背石磺的基础免疫学资料。
  • 图  1  瘤背石磺体腔液中不同类型体腔细胞的显微形态(×40)

    Fig.  1  The micro-morphology of different coelomocytes in coelomic fluid of Onchidium struma (×40)

    图  2  瘤背石磺体腔液中不同类型体腔细胞的密度

    Fig.  2  Density of different coelomocytes in coelomic fluid of Onchidium struma

    图  3  瘤背石磺体腔细胞Unigenes和Transcript长度分布

    Fig.  3  Length distribution of all Unigenes and Transcript from coelomocytes in Onchidium struma

    图  4  瘤背石磺体腔细胞转录本序列与数据库中已知序列比对结果

    a. 基于Nr分析每个Unigenes的相似性分布;b. E值分布;c. 物种相似率分布

    Fig.  4  Comparison of transcriptomic sequences from coelomocytes in Onchidium struma with the known sequences from different species in database

    a. Analyze the similarity distribution of each unigenes based on Nr database; b. E-value distribution; c. species similarity rate distribution for each Unigene

    图  5  瘤背石磺体腔细Unigenes的GO功能注释

    Fig.  5  Gene ontology (GO) functional annotation of Unigenes from coelomocytes in Onchidium struma

    图  6  瘤背石磺体腔细胞Unigenes的COG统计图

    Fig.  6  Cluster of orthologous group (COG) functional annotation of Unigenes from coelomocytes in Onchidium struma

    图  7  瘤背石磺体腔细胞Unigenes的KEGG注释分类

    Fig.  7  KEGG annotation of the assembled Unigenes from coelomocytes in Onchidium struma

    表  1  瘤背石磺体腔细胞6个文库的序列统计

    Tab.  1  Statistics of transcriptomic sequences from six libraries formed from coelomocytes of Onchidium struma

    样本原始测序数据过滤后数据过滤后
    碱基数
    错误率/
    %
    Q20值/
    %
    Q30值/
    %
    GC含量/
    %
    C_156 905 46655 196 2408.28G0.0397.4592.8239.69
    C_253 789 34852 602 1147.89G0.0397.5492.9640.95
    C_352 255 81649 929 9427.49G0.0397.5492.9739.96
    C_455 152 33253 717 1168.06G0.0397.6193.1340.92
    C_555 111 05253 356 1608.00G0.0397.2792.3941.54
    C_655 545 30453 926 2408.09G0.0397.6693.2440.93
    均值54 793 22053 121 3027.97G0.0397.5192.9140.66
    下载: 导出CSV
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  • 收稿日期:  2021-04-28
  • 修回日期:  2021-11-19
  • 网络出版日期:  2021-12-23
  • 刊出日期:  2022-02-01

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