瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析

吕庭莉; 杲绍强; 张虎; 张易婵; 陈鹏; 王嘉伟; 乔帼; 杨伯平; 张明明

doi:10.12284/hyxb2022066

瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析

doi: 10.12284/hyxb2022066

吕庭莉^{1, 2,},
杲绍强³,
张虎⁴,
张易婵²,
陈鹏^{1, 2},
王嘉伟^{1, 2},
乔帼²,
杨伯平⁵,
张明明^2, ,

1.
大连海洋大学水产与生命学院，辽宁大连 116023
2.
盐城工学院海洋与生物工程学院，江苏盐城 224051
3.
邳州市新河镇农业技术推广服务中心，江苏徐州 221313
4.
江苏省海洋水产研究所，江苏南通 226007
5.
盐城工学院电气工程学院，江苏盐城 224051

基金项目: 国家自然科学基金面上项目(32071615)；江苏省水生生物资源重大专项(ZYHB16-5)。

详细信息

作者简介:
吕庭莉（1996-），女，江苏省徐州市人，研究方向为水产养殖。E-mail：1207972508@qq.com

通讯作者:
张明明，男，博士，副教授，研究方向为生态学和物种资源保护。E-mail：zhangmingm44ok@163.com

中图分类号: S917.4
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- 文章访问数: 491
- HTML全文浏览量: 229
- PDF下载量: 29
- 被引次数: 0
出版历程
- 收稿日期: 2021-04-28
- 修回日期: 2021-11-19
- 网络出版日期: 2021-12-23
- 刊出日期: 2022-02-01

Study on cell classificatioin, immune-related enzymatic characteristics and preliminary transcriptome of coelomocytes in Onchidium struma

Lü Tingli^{1, 2
,},
Gao Shaoqiang³,
Zhang Hu⁴,
Zhang Yichan²,
Chen Peng^{1, 2},
Wang Jiawei^{1, 2},
Qiao Guo²,
Yang Boping⁵,
Zhang Mingming^{2
, ,}

1.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
2.
College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China
3.
Xinhe Town Agro-Tech Extension and Service Center, Xuzhou 221313, China
4.
Jiangsu Marine Fisheries Research Institute, Nantong 226007, China
5.
College of Electrical Engineering, Yancheng Institute of Technology, Yancheng 224051, China

摘要

摘要: 瘤背石磺（Onchidium struma）广泛分布于潮间带和潮下带，是海洋生物向陆地生物辐射进化的重要代表，也是环境监测的指示生物。为丰富瘤背石磺的基础免疫学资料，本文研究了瘤背石磺体腔细胞的类别、细胞密度和体腔液免疫相关酶活性，并通过RNA-Seq测序获得体腔细胞转录本，进行生物信息学分析。结果表明，瘤背石磺主要有变形细胞、球形细胞和色素细胞3种不同类型的体腔细胞，其中，球形细胞数量最多，色素细胞数量最少；在无任何外源刺激下，体腔液内酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶和酚氧化酶等抗氧化酶和免疫相关酶均具有活性，且超氧化物歧化酶和酚氧化酶活性最高；转录组学分析显示，体腔细胞中共组装出63 097个Unigenes，KEGG将这些Unigenes主要注释到“信号转导” “运输和分解代谢” “内分泌系统” “翻译” “折叠、分选和降解”等信号通路。研究结果将利于进一步揭示瘤背石磺的体腔液及其体腔细胞在机体免疫防御过程中的作用，丰富瘤背石磺的基础免疫学资料。
- 瘤背石磺 /
- 体腔液 /
- 体腔细胞 /
- 免疫相关酶 /
- 转录组分析
Abstract: Onchidium struma is widely distributed in intertidal zone and subtidal zone, and considered as a representative species of evolution from ocean to land and an indicator organism for evaluation of environment quality. To enrich the basic immunological data of O. struma, the classification of coelomocytes, cell density and immune-related enzyme activity of the coelomic fluid are studied, and also transcriptome analysis is performed by RNA-Seq sequencing. The results show that there are three different types of coelomocytes, including amoebocytes, spherulocytes and chromatocytes. The number of coelomocytes from more to less are spherulocytes, amoebocytes, chromatocytes. The higher enzyme activities including acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) and polyphenol oxidase (PO), are detected in coelomic fluids. The activities of SOD and PO are highest. Based on De novo transcriptome analysis, a total of 63 097 unigenes are assembled. KEGG annotations of these unigenes are mostly enriched in “signal transduction” “transport and catabolism” “endocrine system” “translation” “folding, sorting and degradation”, and signalling pathways. The present results will be helpful to further reveal the important role of the body cavity fluid and body coelomocytes in the host immune defense, and enrich the basic immunological data of O. struma.
- Onchidium struma /
- coelomic fluid /
- coelomocytes /
- immune-related enzymes /
- transcriptome analysis

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图 1 瘤背石磺体腔液中不同类型体腔细胞的显微形态（×40）

Fig. 1 The micro-morphology of different coelomocytes in coelomic fluid of Onchidium struma (×40)

下载: 全尺寸图片幻灯片

图 2 瘤背石磺体腔液中不同类型体腔细胞的密度

Fig. 2 Density of different coelomocytes in coelomic fluid of Onchidium struma

下载: 全尺寸图片幻灯片

图 3 瘤背石磺体腔细胞Unigenes和Transcript长度分布

Fig. 3 Length distribution of all Unigenes and Transcript from coelomocytes in Onchidium struma

下载: 全尺寸图片幻灯片

图 4 瘤背石磺体腔细胞转录本序列与数据库中已知序列比对结果

a. 基于Nr分析每个Unigenes的相似性分布；b. E值分布；c. 物种相似率分布

Fig. 4 Comparison of transcriptomic sequences from coelomocytes in Onchidium struma with the known sequences from different species in database

a. Analyze the similarity distribution of each unigenes based on Nr database; b. E-value distribution; c. species similarity rate distribution for each Unigene

下载: 全尺寸图片幻灯片

图 5 瘤背石磺体腔细Unigenes的GO功能注释

Fig. 5 Gene ontology (GO) functional annotation of Unigenes from coelomocytes in Onchidium struma

下载: 全尺寸图片幻灯片

图 6 瘤背石磺体腔细胞Unigenes的COG统计图

Fig. 6 Cluster of orthologous group (COG) functional annotation of Unigenes from coelomocytes in Onchidium struma

下载: 全尺寸图片幻灯片

图 7 瘤背石磺体腔细胞Unigenes的KEGG注释分类

Fig. 7 KEGG annotation of the assembled Unigenes from coelomocytes in Onchidium struma

下载: 全尺寸图片幻灯片

表 1 瘤背石磺体腔细胞6个文库的序列统计

Tab. 1 Statistics of transcriptomic sequences from six libraries formed from coelomocytes of Onchidium struma

样本	原始测序数据	过滤后数据	过滤后碱基数	错误率/ %	Q20值/ %	Q30值/ %	GC含量/ %
C_1	56 905 466	55 196 240	8.28G	0.03	97.45	92.82	39.69
C_2	53 789 348	52 602 114	7.89G	0.03	97.54	92.96	40.95
C_3	52 255 816	49 929 942	7.49G	0.03	97.54	92.97	39.96
C_4	55 152 332	53 717 116	8.06G	0.03	97.61	93.13	40.92
C_5	55 111 052	53 356 160	8.00G	0.03	97.27	92.39	41.54
C_6	55 545 304	53 926 240	8.09G	0.03	97.66	93.24	40.93
均值	54 793 220	53 121 302	7.97G	0.03	97.51	92.91	40.66

下载: 导出CSV

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