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低氧胁迫对军曹鱼幼鱼免疫相关基因转录水平表达的影响

王维政 杨林桐 杨二军 谢瑞涛 陈刚 黄建盛

王维政,杨林桐,杨二军,等. 低氧胁迫对军曹鱼幼鱼免疫相关基因转录水平表达的影响[J]. 海洋学报,2021,43(9):92–101 doi: 10.12284/hyxb2021116
引用本文: 王维政,杨林桐,杨二军,等. 低氧胁迫对军曹鱼幼鱼免疫相关基因转录水平表达的影响[J]. 海洋学报,2021,43(9):92–101 doi: 10.12284/hyxb2021116
Wang Weizheng,Yang Lintong,Yang Erjun, et al. Transcription level of immune related genes of juvenile cobia (Rachycentron canadum) under hypoxia stress[J]. Haiyang Xuebao,2021, 43(9):92–101 doi: 10.12284/hyxb2021116
Citation: Wang Weizheng,Yang Lintong,Yang Erjun, et al. Transcription level of immune related genes of juvenile cobia (Rachycentron canadum) under hypoxia stress[J]. Haiyang Xuebao,2021, 43(9):92–101 doi: 10.12284/hyxb2021116

低氧胁迫对军曹鱼幼鱼免疫相关基因转录水平表达的影响

doi: 10.12284/hyxb2021116
基金项目: 财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-47);南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-06)
详细信息
    作者简介:

    王维政(1995-),男,广东省广州市人,主要研究方向为海水鱼类养殖生理生态学。E-mail:1029588896@qq.com

    通讯作者:

    黄建盛,副教授,主要研究方向海水鱼养殖生理生态学。E-mail:huangjs@gdou.edu.cn

  • 中图分类号: Q786

Transcription level of immune related genes of juvenile cobia (Rachycentron canadum) under hypoxia stress

  • 摘要: 为研究低氧胁迫对军曹鱼幼鱼免疫功能的影响,将幼鱼暴露于溶氧浓度为(3.15±0.21) mg/L的水体28 d,测定不同时间点肿瘤坏死因子α (TNFα)、肿瘤坏死因子α诱导蛋白3 (TNFAIP3)、白细胞介素1β (IL-1β)、白细胞介素1受体2 (IL-1R2)、白细胞介素17C (IL-17C)和热休克蛋白70 (HSP70)等免疫相关基因在幼鱼鳃、肝脏、肠道和脾脏中的转录水平表达量。结果显示:(1)在幼鱼的鳃中,TNFα 和 IL-1R2 基因转录水平表达量在胁迫1 d和14 d时极显著低于对照组(p<0.01),胁迫 28 d则分别表现为极显著(p<0.01)和显著下降(p<0.05);TNFAIP3在胁迫1 d时极显著升高(p<0.01),在胁迫7 d和14 d时则分别表现为显著(p<0.05)和极显著(p<0.01)下降;IL-1β在胁迫1 d时极显著下降(p<0.01)后极显著升高 (p<0.01);IL-17C在胁迫7 d和14 d时极显著下降(p<0.01);HSP70 在胁迫1 d时显著下降(p<0.05),在胁迫14 d和28 d时均极显著降低(p<0.01);(2)在肝脏中,TNFα 和 IL-1R2 基因转录水平表达量在胁迫1 d和28 d时显著下降(p<0.05),在胁迫14 d时则极显著降低(p<0.01);TNFAIP3在胁迫1 d时极显著上升(p<0.01),在胁迫14 d和28 d时则极显著下降(p<0.01);IL-1β在所有胁迫时间点均极显著上升(p<0.01);IL-17C 在胁迫7 d、14 d和28 d时均极显著低于对照水平(p<0.01);HSP70基因表达量持续上升,并于胁迫28 d时达到顶峰(p<0.01);(3)在肠道中,TNFα、IL-1β、IL-1R2、IL-17C和HSP70基因转录水平表达量在胁迫的所有时间点均极显著高于对照组(p<0.01),TNFAIP3在胁迫1 d时表达量极显著升高(p<0.01)后下降,在胁迫7 d和14 d时分别与对照组具有显著(p<0.05)和极显著差异(p<0.01);(4)在脾脏中,TNFα和IL-17C 基因转录水平表达量在胁迫1 d、7 d和14 d时极显著下降(p<0.01),在胁迫28 d时则显著降低(p<0.05);IL-1β在胁迫1 d和28 d时极显著下降(p<0.01),在胁迫7 d和14 d时则显著降低(p<0.05);IL-1R2在胁迫1 d、7 d和28 d时极显著下降(p <0.01),在胁迫14 d时则显著下降(p<0.05);HSP70基因表达量在胁迫1 d、7 d和28 d时均极显著下降(p<0.01)。研究结果表明,28 d的低氧胁迫后,军曹鱼幼鱼免疫基因的转录表达水平发生显著性变化,预示长时间的低氧可能抑制了军曹鱼的免疫功能,引发肠道炎症,并增加了军曹鱼感染病原菌的风险。
  • 图  1  低氧胁迫后军曹鱼幼鱼鳃组织TNFα(a)、TNFAIP3(b)、IL-1β(c)、IL-1R2(d)、IL-17C(e)和HSP70(f)基因的相对表达量

    *表示显著差异(p<0.05),**表示极显著差异(p<0.01)

    Fig.  1  Relative expression of TNFα (a), TNFAIP3 (b), IL-1β (c), IL-1R2 (d), IL-17C (e) and HSP70 (f) genes in gill of juvenile cobia after hypoxia

    *Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

    图  2  低氧胁迫后军曹鱼幼鱼肝组织TNFα(a)、TNFAIP3(b)、IL-1β(c)、IL-1R2(d)、IL-17C(e)和HSP70(f)基因的相对表达量

    *表示显著差异(p<0.05),**表示极显著差异(p<0.01)

    Fig.  2  Relative expression of TNFα (a), TNFAIP3 (b), IL-1β (c), IL-1R2 (d), IL-17C (e) and HSP70 (f) genes in liver of juvenile cobia after hypoxia

    *Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

    图  3  低氧胁迫后军曹鱼幼鱼肠组织TNFα(a)、TNFAIP3(b)、IL-1β(c)、IL-1R2(d)、IL-17C(e)和HSP70(f)基因的相对表达量

    *表示显著差异(p<0.05),**表示极显著差异(p<0.01)

    Fig.  3  Relative expression of TNFα (a), TNFAIP3 (b), IL-1β (c), IL-1R2 (d), IL-17C (e) and HSP70 (f) genes in intestine of juvenile cobia after hypoxia

    *Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

    图  4  低氧胁迫后军曹鱼幼鱼脾组织TNFα(a)、TNFAIP3(b)、IL-1β(c)、IL-1R2(d)、IL-17C(e)和HSP70(f)基因的相对表达量

    *表示显著差异(p<0.05),**表示极显著差异(p<0.01)

    Fig.  4  Relative expression of TNFα (a), TNFAIP3 (b), IL-1β (c), IL-1R2 (d), IL-17C (e) and HSP70 (f) genes in spleen of juvenile cobia after hypoxia

    *Shows significant difference (p<0.05), **shows extremely significant difference (p<0.01)

    表  1  本研究所用引物序列

    Tab.  1  Primer sequences used in this study

    引物名称序列(5’−3’)用途
    TNFα-FGCTGGAGTGGAAGAACGGTCAAqRT-PCR
    TNFα-RCTTGCCGCCTATGGAGTCTGAGqRT-PCR
    TNFAIP3-FTCCAGAATGCCTCACGGTGTCqRT-PCR
    TNFAIP3-RAGTGTGGTGTGCGATGTGTCAqRT-PCR
    IL-1β-FGCAGAGAATCGGCACAGACAqRT-PCR
    IL-1β-RGAAGGTTCGGTAGCGGTTGTqRT-PCR
    IL-1R2-FACCAAGGAGGAAGACACGAAGTqRT-PCR
    IL-1R2-RTGCCGAGCCAGGATGTAATCAqRT-PCR
    IL-17C-FGCCAAAGAAATGCGAGGAGATGqRT-PCR
    IL-17C-RACAGAGGCACTCAGCGAAGAqRT-PCR
    HSP70-FTCACTGGAGTCCTACGCCTACqRT-PCR
    HSP70-RGCTGATGCTCATACTCGTCCTTqRT-PCR
    β-actin-FAGGGAAATTGTGCGTGACqRT-PCR
    β-asctin-RAGGCAGCTCGTAGCTCTTqRT-PCR
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  • 收稿日期:  2021-01-30
  • 修回日期:  2021-04-12
  • 网络出版日期:  2021-05-12
  • 刊出日期:  2021-09-06

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