Digital gene expression profiling analysis of <em>Pyropia haitanensis</em> under high temperature stress

Lai Xiaojuan; Yan Xiaojun; Yang Rui; Luo Qijun; Chen Haimin

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Lai Xiaojuan, Yan Xiaojun, Yang Rui, Luo Qijun, Chen Haimin. Digital gene expression profiling analysis of Pyropia haitanensis under high temperature stress[J]. Haiyang Xuebao, 2014, 36(6): 104-111.

Citation:

Lai Xiaojuan, Yan Xiaojun, Yang Rui, Luo Qijun, Chen Haimin. Digital gene expression profiling analysis of Pyropia haitanensis under high temperature stress[J]. Haiyang Xuebao, 2014, 36(6): 104-111.

Lai Xiaojuan, Yan Xiaojun, Yang Rui, Luo Qijun, Chen Haimin. Digital gene expression profiling analysis of Pyropia haitanensis under high temperature stress[J]. Haiyang Xuebao, 2014, 36(6): 104-111.

Citation:

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Digital gene expression profiling analysis of Pyropia haitanensis under high temperature stress

Marine Biotechnology Laboratory, Key Laboratory of Applied Marine Biotechnology, Ministry of Educaltion, Ningbo University, Ningbo 315211, China

Received Date: 2013-06-05

Abstract

Abstract

Pyropia haitanensis is an economically important red alga cultivated in China. As an intertidal alga,P. haitanensis developed various and highly effective strategies to overcome those environmental stressors,such as temperature fluctuation and osmotic shock. In this research,digital gene expression profiling (DGE) technique based on high throughput sequencing was used to analysis the gene expression difference of P. haitanensis under high temperature stress. To validate the DGE data,eight genes were selected for real-time quantitative PCR (QRT-PCR) analysis. The expression level of high-temperature response gene hsp70 was particularly investigated. Results indicated that 256 unigenes including HSP,ribosomal protein L12,elongation factor EF-Tu and some photosynthesis-related genes were up-regulated,3 820 unigenes involved in anabolism and metabolism of nucleic acid,protein and carbohydrate were down-regulated. Gene Ontology analysis indicated that differentially expressed genes mainly involved in reproduction and developmental process,exercised the functions of catalytic activity and ligase activity,and located in membrane-bounded organelle such as plastid. Pathway enrichment analysis showed that these genes distributed in 107 pathways. Down-regulated genes were significantly enriched in mRNA surveillance and RNA transport pathway,while up-regulated genes were partially enriched in protein processing in endoplasmic reticulum,RNA degradation and photosynthesis pathway. QRT-PCR confirmed the accuracy of DGE,and the positive response of hsp70 to high temperature was also demonstrated. In summary,DGE results indicated that under high temperature stress,primary metabolism,biosynthesis,energy synthesis,and carbon assimilation of P. haitanensis were weakened,whereas,the rescue strategies were activated.
- Pyropia haitanensis,
- high temperature stress,
- digital gene expression profiling (DGE),
- differentially expressed gene,
- response

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