Adhesion and colonization of fluorescence labeled pathogenic <em>Vibrio fluvialis</em> to intestine of <em>Einephelus awoara</em>

QIN Yingxue; ZHAO Minhui; SU Yongquan; WANG Jun; ZHANG Xiaodong; WANG Xiaolu; YAN Qingpi

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Article Navigation > Haiyang Xuebao > 2012 > 34(6): 170-176

QIN Yingxue, ZHAO Minhui, SU Yongquan, WANG Jun, ZHANG Xiaodong, WANG Xiaolu, YAN Qingpi. Adhesion and colonization of fluorescence labeled pathogenic Vibrio fluvialis to intestine of Einephelus awoara[J]. Haiyang Xuebao, 2012, 34(6): 170-176.

Citation:

QIN Yingxue, ZHAO Minhui, SU Yongquan, WANG Jun, ZHANG Xiaodong, WANG Xiaolu, YAN Qingpi. Adhesion and colonization of fluorescence labeled pathogenic Vibrio fluvialis to intestine of Einephelus awoara[J]. Haiyang Xuebao, 2012, 34(6): 170-176.

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Adhesion and colonization of fluorescence labeled pathogenic Vibrio fluvialis to intestine of Einephelus awoara

1.
Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture, Jimei University, Xiamen 361021, China
2.
College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China

Received Date: 2011-04-03
Rev Recd Date: 2012-06-15

Abstract

Abstract

The fluorescence labeled bacterium EGFP-Vibrio fluvialis were introduced into the digestive tract of Einephelus awoara by intragastic injection in order to trace this bacterium colonization and its effect on digestive enzyme in fish intestine. The results showed that labeled bacterium could be detected in forgut and midgut 12 h after intragastic injection,and the number of the labeled bacteria in forgut reached a peak value 7.1×10⁵cfu/g 24 h after intragastic injection,in midgut the peak value 5.39×10⁵ cfu/g was observed 36 h after intragastic injection,in hindgut the labeled bacterium was not detected until 24 h after intragastic injection and the peak value was only 2.44×10⁴ cfu/g. This result indicated V. fluvialis tended to adhere in forgut and midgut. Bacterium was still detected in forgut and midgut 96 h after intragastic injection,but not detected in hindgut. This indicated that forgut and midgut were the main location for bacteria colonization. The results also showed that the activity of alkaline protease and amylase in forgut and midgut and lipase in hindgut were significantly decreased after V. fluvialis colonization. This result implied that V. fluvialis could affect activity of digestive enzyme in the fish gut after colonization,which might be one of the pathogenesis of V. fluvialis.
- Vibrio fluvialis,
- Epinephelus awoara,adhesion,
- colonization,

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References(24)

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