Effects of environmental factors on the pGTF gene expression of Antarctic bacterium <em>Pseudoalteromonas</em> sp.S-15-13

LI Jiang; TAN Jiaojiao

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LI Jiang, TAN Jiaojiao. Effects of environmental factors on the pGTF gene expression of Antarctic bacterium Pseudoalteromonas sp.S-15-13[J]. Haiyang Xuebao, 2012, 34(5): 154-160.

Citation:

LI Jiang, TAN Jiaojiao. Effects of environmental factors on the pGTF gene expression of Antarctic bacterium Pseudoalteromonas sp.S-15-13[J]. Haiyang Xuebao, 2012, 34(5): 154-160.

LI Jiang, TAN Jiaojiao. Effects of environmental factors on the pGTF gene expression of Antarctic bacterium Pseudoalteromonas sp.S-15-13[J]. Haiyang Xuebao, 2012, 34(5): 154-160.

Citation:

LI Jiang, TAN Jiaojiao. Effects of environmental factors on the pGTF gene expression of Antarctic bacterium Pseudoalteromonas sp.S-15-13[J]. Haiyang Xuebao, 2012, 34(5): 154-160.

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Effects of environmental factors on the pGTF gene expression of Antarctic bacterium Pseudoalteromonas sp.S-15-13

LI Jiang¹,
TAN Jiaojiao²

1.
Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgarphy, State Oceanic Administration, Qingdao 266061
2.
College of Chemical Engineering, Qingdao University of Science Technology, Qingdao 266042

Received Date: 2011-02-13
Rev Recd Date: 2012-02-09

Abstract

Abstract

The priming glycosyltransferase gene (gtf) was cloned from the Antarctic bacterium Pseudoalteromonas sp.S-15-13,which was isolated from sea ice (62°00'S,68°30'E) samples,in order to explore the role and mechanism of extracellular polysaccharides on the low temperature adaptation of this strain.The effects of environmental factors (such as temperature, treatment with freeze-thaw cycles, salt concentrations and pH values) on gtf expression were studied with real-time PCR techniques.It was shown that the gtf expression level of S-15-13 stimulation at 2℃ was about 1.5 times that grown at 10℃ after 1h; and the gtf expression level of S-15-13 grown at 4 and 10℃ was about 8~12 times that grown at 20℃ after 24 h culture; the expression level of gtf was 3.667 times higher than the untreated sample after two freeze-thaw cycles of Pseudoalteromonas sp.S-15-13; 6.0% NaCl could increase the expression level of gtf nearly four times compared to that of 3.0% NaCl, but when the NaCl concentration reached 9.0 %, the expression level of gtf dropped off sharply; pH changes in the range of pH 5.0~8.0 could promote the expression of gtf: the level of gtf expression at pH 6.0 was 2 times that at pH 7.0.Study on gtf of Pseudoalteromonas sp.S-15-13 may have potential to deepen our understanding of the adaptation mechanism of Antarctic bacteria living in harsh environments.
- Antarctic bacterium Pseudoalteromonas sp.S-15-13,
- Real-time PCR,
- GTF,
- environmental factors

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References(15)

References

曾胤新, 陈波.南极低温微生物研究及应用前景[J].极地研究, 1999, 11(2): 143-152.

KIRST G,WIENCKE C.Ecophysiology of polaralgae[J].J Phyco1, 1995, 31:181-199.

RUSSEL N J.Psychrophilic bacteria-molecular adaptations of membmne lipids[J].Comp Biochem Physio1 A Physio, 1997, 11(3): 489-493.

SELBMANN L, ONOFRI S, FENICE M, et a1.Production and structural characterization of the exopolysaccharide of the Antarctic fungus Phoma herbarum CCFEE 5080 [J].Research in Microbiology, 2002, 153(9): 585-592.

KREMBS C,ENGEL A.Abundance and variability of microorganisms and transparent exopolymer particles across ice-water interface of melting firstyear sea ice in t he Laptev Sea (Arctic) [J].Mar Biol,200,138: 173-185.

KREMBS C,EICKEN H,JUNGE K,et al.High concentrations of exopolymeric substances in Arctic winter sea ice: Implications for the polar ocean carbon cycle and cryoprotection of diatoms[J].Deep-Sea Res,2002,49: 2163-2181.

MORONA J K, MORONA R, PATON J C.Characterization of the locus encoding the Streptomyces pneumoniae type19F capsular polysaccharide biosynthetic pathway[J].Mol Microbiol,1997,23: 751-763.

WHITFIELD C, VALVANO M A.Biosynthesis and expression of cell-surface polysaccharides in Gram-negative bacterial[J].Adv Microbial Physiol,1993,35: 136-246.

BAI L P,JIANG R, SHAN J J,et al.Purification, characterization and functional analysis of asparagines synthetase encoding by ste10 gene in Ebosin biosynthesis of Streptomyces sp.139[J].Enzyme and Microbial Technology,2008, 42: 548-553.

JOLLY L,STINGELE F.Molecular organization and functionality of exopolysaccharide gene clusters in lactic acid bacteria[J].International Dairy Journal, 2001,11: 733-745.

THOMAS D N, DIECKMANN G S.Antarctic sea ice a habitat for extremophiles[J].Science, 2002,295(25): 641-644.

WHITFIELD C,AMOR P A,KOPLIN R.Modulation of the surface architecture Gram-negative bacteria by the action of surface Polymer: lipid A-core ligase and by determinants of polymer chain length[J].Mol Microbiol, 1997, 23: 629-638.

MANCUSO N C, GARON S, BOWMAN J P, et al.Production of exopolysaccharides by Antarctic marine bacterial isolates[J].J Appl Microbiol, 2004, 96: 1057-1066.

李江,何培青,陈靠山,等.南极适冷菌 Pseudoalteromonas sp.S-15-13胞外多糖低温保护作用的研究[J].海洋科学进展,2007, 25(2):215-219.

SULLIVAN C W,PALMISANO A C.Sea ice microbial communities: distribution, abundance and diversity of ice bacteria in McMurdo Sound, Antarctica, in 1980[J].Appl Environ Microbiol, 1984,47: 788-795.

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