Effects of seawater temperature on the reproductive activity of the amphioxus,<em>Branchiostoma belcheri</em> and its endocrine mechanism

WENG Youzhu; LIU Zhigang; FANG Yongqiang; XUE Wenling

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Article Navigation > Haiyang Xuebao > 2012 > 34(4): 163-171

WENG Youzhu, LIU Zhigang, FANG Yongqiang, XUE Wenling. Effects of seawater temperature on the reproductive activity of the amphioxus,Branchiostoma belcheri and its endocrine mechanism[J]. Haiyang Xuebao, 2012, 34(4): 163-171.

Citation:

WENG Youzhu, LIU Zhigang, FANG Yongqiang, XUE Wenling. Effects of seawater temperature on the reproductive activity of the amphioxus,Branchiostoma belcheri and its endocrine mechanism[J]. Haiyang Xuebao, 2012, 34(4): 163-171.

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Effects of seawater temperature on the reproductive activity of the amphioxus,Branchiostoma belcheri and its endocrine mechanism

Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2011-08-01
Rev Recd Date: 2012-02-09

Abstract

Abstract

Effects of different seawater temperature(low temperature group 16℃, middle temperature 22℃ and high temperature 28℃) on sex differentiation and gonadal development in the amphioxus Branchiostoma belcheri without gonads and on the spawning and spermiation activity in mature amphioxus Branchiostoma belcheri at the breeding season were studied using the chronic experiment method.The immunological recognition of several reproduction-related hormones in the reproductive endocrine regulation organ(namely, Brain-hatschek’s pit-gonad) of amphioxus at different development stages was studied by immunohistochemistry and the testosterone and 17β-estradiol contents in gonads of amphioxus were detected by magnetic enzyme immunoassay to assay and investigate the endocrine mechanism of the effects of seawater temperature on reproductive activity of amphioxus. The results indicated that low sea water temperature promoted gonadal differentiation and development towards female in amphioxus, and the ratio of female to male was 4.2:1. There were no differences in sex ratio in the middle temperature group and in the high temperature group, but high seawater temperature could promote testis development and spermatogenic activity. The high temperature 28℃ was suitable for the reproductive activity of amphioxus. At the same time, the endocrine mechanism of the influence of temperature on the sex differentiation, gonadal development and reproductive activity in amphioxus was suggested by the results of immunohistochemistry and magnetic enzyme immunoassay as followed: Firstly, the seawater temperature may stimulate GnRH release from nervous system (brain vesicle) via skin receptor or chemical receptor of Hatschek’s pit. Then, GnRH may promote LH secretion from Hatschek’s pit. Finally, LH may stimulate the formation and secretion of sex steroid hormones in the ovary and testis, and thus initiate gonad development and maturation as well as reproductive activity.In addition,aromatase in the brain may be involved in the sex differentiation of amphioxus.
- Branchiostoma belcheri,
- temperature,
- sex differentiation,
- reproductive control axis,
- aromatase

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References

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