Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent

ZHENG Li; CUI Zhisong; GAO Wei; HAN Bin; YANG Baijuan; WANG Shaoliang; LI Qian; ZHOU Wenjun; ZHANG Kuiying; CAO Lei

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Article Navigation > Haiyang Xuebao > 2012 > 34(3): 163-172

ZHENG Li, CUI Zhisong, GAO Wei, HAN Bin, YANG Baijuan, WANG Shaoliang, LI Qian, ZHOU Wenjun, ZHANG Kuiying, CAO Lei. Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent[J]. Haiyang Xuebao, 2012, 34(3): 163-172.

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Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent

First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2011-04-19
Rev Recd Date: 2011-09-28

Abstract

Abstract

A marine oil-degrading bacterial consortium DC10 was prepared to an oil-degrading agent and further applied to the bioremediation of crude oil-contaminated Dalian beach. Field studies were carried out at the intertidal zone and supratidal zone respectively, and the effects of the oil-degrading agent were investigated by evaluating the decline of C17/hopane, C18/hopane, total petroleum alkanes and total petroleum aromatics.The oil-degrading consortium DC10 exhibited much higher degradation rates than the single degrader comprising consortium DC10 under laboratory conditions. Compared to the sterile control, the degradation rates of consortium DC10 increased by 60% in 7 days, and most alkanes and aromatics in oil were disappeared. The oil-degrading agent supplemented with essential nutrient salts was proved to be effective in the field study on the bioremediation of oil-contaminated Dalian beach. At the intertidal zone plot, the C17/hopane and C18/hopane degradation rates of bioremediation treatment increased by approximately 40% and 30% in 12 d, respectively, compared to the natural weathering. While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 80% and 72% in 12 d. In the case of supratidal zone plot, the C17/hopane and C18/hopane degradation rates of bioremediation treatment was only slightly higher than natural weathering in 85 d. While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 30% and 20% in 85 d, respectively.
- bioremediation,
- marine oil spill,
- petroleum-degrading bacteria

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References

YEMASHOVA N A, MURYGINA V P, ZHUKOV D V, et al.Biodeterioration of crude oil and oil derived products: a review[J]. Rev Environ Sci Biotechnol, 2007, 6(4): 315-337.

REIA Hosokawa, MOTONORI Nagai, MASAAKI Morikawa, et al. Autochthonous bioaugmentation and its possible application to oil spills[J].WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 25(9): 1519-1528.

XIA W X, ZHENG X L, LI J C, et al.Degradation of crude oil by indigenous microorganisms supplemented with nutrients[J]. J Envir Sci, 2005, 17(4), 659-661.

LI H, ZHAO Q, BOUFADEL M C, VENOSA A D, A universal nutrient application strategy for the bioremediation of oil-polluted beaches. Marine Pollut Bull 2007, 54(8):1146-1161.

RON E, ROSENBERG E. Role of Fertilizers: Biostimulation. Handbook of hydrocarbon and lipid microbiology, 2010, Part 24, 2521-2529, DOI: 10.1007/978-3-540-77587-4-184.

BRAGG J R, PRINCE R C, HARNER E J, et al.Effectiveness of bioremediation for the Exxon Valdez oil spill[J]. Nature, 1994, 368: 413-418.

NIKOLOPOULOU M, KALOGERAKIS N.Biostimulation Strategies for Enhanced Bioremediation of Marine Oil Spills Including Chronic Pollution. Handbook of hydrocarbon and lipid microbiology, 2010, Part 24, 2501-2504, DOI: 10.1007/978-3-540-77587-4_187

TSUTSUMI H, KONO M, TAKAI K, et al.Bioremediation on the shore after an oil spill from the Nakhodka in the Sea of Japan: Ⅲ.Field test of a bioremediation agent with microbiological cultures for the treatment of an oil spill[J]. Mar Pollu Bull, 2000, 40(4), 320-324.

XIA W X, LI J C, ZHENG X L, et al.Enhanced biodegradation of diesel oil in seawater supplemented with nutrients[J]. Engin Life Sci, 2006, 6(1): 80-85.

HII Y S, LAW A T, SHAZILL N A M, et al. Biodegradation of Tapis blended crude oil in marine sediment by a consortium of symbiotic bacteria[J]. 2009, 63(2):142-150.

韩平, 郑立, 崔志松, 等. 胜利油田滩涂区石油降解菌的筛选、鉴定及其多样性分析[J]. 应用生态学报, 2009, 20(5): 1202-1208.

崔志松, 郑立, 杨佰娟, 等,. 两种海洋专性解烃菌降解石油的协同效应[J]. 微生物学报, 2010, 50(3): 350-359.

OH Y S, SIM D S, KIM S J. Effects of nutrients on crude oil biodegradation in the upper intertidal zone[J]. Mar Pollut Bull, 2001, 42(12), 1367-1372.

PRINCE R C, ELMENDORF D L, LUTE J R, et al.17α (H),21β (H)-Hopane as a conserved internal marker for estimating the biodegradation of crude oil[J]. Environ Sci Technol, 1994, 28(1): 142-145.

OH Y S, CHOI W Y, LEE Y H, et al.Biological treatment of oil-contaminated sand: comparison of oil degradation based on thin-layer chromatography/flame ionization detector and respirometric analysis[J]. Biotech Let, 2000, 22(7): 595-598.

MARIA N, NICOLAS K. Biostimulation strategies for fresh and chronically pollutedmarine environments with petroleum hydrocarbons[J]. Journal of Chemical Technology and Biotechnology, 2009, 84(6): 802-807.

NIKOLOPOULOUA M, KALOGERAKIS, N. Enhanced bioremediation of crude oil utilizing lipophilic fertilizers combined with biosurfactants and molasses[J]. Mar Pollut Bull, 2008, 56, 1855-1861.

LEE K, TREMBLAY GH, COBANLI SE.Bioremediation of oiled beach sediments: Assessment of inorganic and organic fertilizers //Proceedings of 1995 Oil Spill Conference.Washington, DC: American Petroleum Institute, 1995:107-113.

KNEZEVICH V O, KOREN E, RON Z, et al. Petroleum bioremediation in seawater using guano as the fertilizer[J]. Bioremediation J, 2006, 10:83-91.

DELAUNE R, LAN D, WRIGHT L. Projected Impact of Deepwater HorizonOil Spill on U.S. Gulf Coast Wetlands[J]. Soil Sci Soc Am J, 2011, 75(5):1-11.

SWANNELL R P J, LEE K, MCDONAGH M. Field evaluations of marine oil spill bioremediation[J]. Microbiological Reviews, 1996, 60(2), 342-365.

ZHU X, VENOSA A D, SUIDAN M T. Literature review on the use of commercial bioremediation agents for cleanup of oil-contaminated estuarine environments.EPA.2004, EPA/600/R-04/075.

胥九兵, 迟建国, 邱维忠, 等, 石油降解菌剂的研制及其在石油污染土壤修复中的应用[J]. 生物加工过程, 2009, 7(6): 21-24.

黄廷林, 徐金兰, 唐智新, 等,生物菌剂对石油污染土壤生物修复作用的研究[J]. 环境科学, 2009, 30(6): 838-1843.

YOUNG-SOOK O, DOO-SEUP S, SANG-JIN K. Effects of nutrients on crude oil biodegeadation in the upper intertidal zone[J], Marine Pollution Bulletin, 2001, 42(12), 1367-1372.

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