Responses of nucleic acid and protein content in <em>Prorocentrum donghaiense </em>Lu to different nitrogen and phosphorus concentrations

LIU Yun; SONG Xiuxian; CAO Xihua; YUAN Yongquan; YU Zhiming

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LIU Yun, SONG Xiuxian, CAO Xihua, YUAN Yongquan, YU Zhiming. Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations[J]. Haiyang Xuebao, 2012, 34(2): 153-161.

Citation:

LIU Yun, SONG Xiuxian, CAO Xihua, YUAN Yongquan, YU Zhiming. Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations[J]. Haiyang Xuebao, 2012, 34(2): 153-161.

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Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations

1.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School, Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2011-06-01
Rev Recd Date: 2011-09-26

Abstract

Abstract

Changes in growth, cellular DNA, RNA and total protein content related to nitrogen(N) and phosphorus(P) concentrations were studied in a marine dinoflagellate Prorocentrum donghaiense Lugrown in batch cultures. The results show that the growth of P. donghaiense is significantly affected by N and P concentrations. The ultimate biomass depended on the initial N and P concentrations. The maximum growth varied distinctly under different N or P treatments(P<0.05). DNA content had significant correlation with N concentrations in the cultures but not with P. RNA content was strongly associated with both N and P concentrations(P<0.01). Different growth stages could also change cellular RNA content. The RNA content decreased along with the extension of experimental time.RNA/DNA ratio had significant correlation with the N and P concentrations. There appeared to be a linear relationship between the growth rate and the ratio of RNA/DNA. Cellular total protein content was significantly affected by N concentrations. P deficiency could also reduce cellular protein content.
- Prorocentrum donghaiense Lu,
- DNA,
- RNA,
- RNA/DNA,
- protein,
- growth rate

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References(30)

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