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SHEN Xin, TIAN Mei, ZHU Changbao, LIU Huilian, ZHAO Fangqing. Determination of Membranipora grandicella complete mitochondrial genome sequence based on next-generation sequencing technology[J]. Haiyang Xuebao, 2012, 34(2): 136-142.
Citation: SHEN Xin, TIAN Mei, ZHU Changbao, LIU Huilian, ZHAO Fangqing. Determination of Membranipora grandicella complete mitochondrial genome sequence based on next-generation sequencing technology[J]. Haiyang Xuebao, 2012, 34(2): 136-142.

Determination of Membranipora grandicella complete mitochondrial genome sequence based on next-generation sequencing technology

  • Received Date: 2011-06-26
  • Rev Recd Date: 2011-09-26
  • Traditionally, the mitochondrial genome sequences were usually obtained by shotgun or primers-walking combination with long PCR. The next-generation sequencing technologies are developing fastly in recent years, such as 454, Solexa and SOLiD sequencing platform. Membranipora grandicella complete mitochondrial genome sequence was determined based on next-generation high-throughput sequencing technology (Solexa) and nested PCR amplification. The complete mitochondrial genome of M. grandicella is 15,861 bp in length, which is the first representative from the suborder Malacostegina (Bryozoa), and is relatively larger when compared with mtDNAs of other study bryozoans. The mitochondrial genome contains 13 protein-coding genes, two ribosomal RNAs and 20 transfer RNAs. Compared with other study bryozoans, gene arrangements of five bryozoans mitochondrial genomes are significantly different. Comparison of bryozoans mitochondrial gene arrangements can be important information sources for exploring the phylogenetic relationships within the group.
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