Assessment of<em> Psychrobacter</em> sp. SE6 from the gut of grouper (<em>Epinephelus coioides</em>) as a novel probiont in vitro and in vivo

YANG Hongling; MA Rulong; SUN Yunzhang

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YANG Hongling, MA Rulong, SUN Yunzhang. Assessment of Psychrobacter sp. SE6 from the gut of grouper (Epinephelus coioides) as a novel probiont in vitro and in vivo[J]. Haiyang Xuebao, 2012, 34(2): 129-135.

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YANG Hongling, MA Rulong, SUN Yunzhang. Assessment of Psychrobacter sp. SE6 from the gut of grouper (Epinephelus coioides) as a novel probiont in vitro and in vivo[J]. Haiyang Xuebao, 2012, 34(2): 129-135.

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Assessment of Psychrobacter sp. SE6 from the gut of grouper (Epinephelus coioides) as a novel probiont in vitro and in vivo

1.
Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen 361021, China
2.
Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Xiamen City for Feed Detection and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China

Received Date: 2011-04-09
Rev Recd Date: 2011-08-09

Abstract

Abstract

The antibacterial activity and tolerance to mimic gastrointestinal environments of the probiotic Psychrobacter sp. SE6 isolated from the gut of grouper(Epinephelus coioides)were evaluated in vitro. On the base of the in vitro results, a 60 d feeding trial was conducted to assess the effect of dietary administration(10⁸ CFU/g) of SE6 on the growth, feed utilization and immune responses of E.coioides.In vitro results show that SE6 exhibits antagonistic activity against pathogenic Vibrio parahaemolyticus, V.harveyi, V.metschnikovi and Staphyloccocus aureus.After 2 and 4 h of incubation with artificial gastric juice at pH 4.0, the survival ratio of SE6 is 110.7% and 173%, respectively. After treated with artificial gastric juice at pH 3.0 for 2 h, the survival ratio and the survival count are 1.36% and 1.59×10⁵CFU/cm³, respectively. However, SE6 cannot survive in artificial gastric juice at pH 2.0.Psychrobacter sp. SE6 exhibits good tolerance to artificial intestinal juice (different concentrations of bile salt and trypsin), the survival ratios of SE6 are 89.17%, 88.33%, 77.17% and 12.33% respectively after incubated in artificial intestinal juice with 0.1%, 0.3%, 0.5% and 0.7% bile salt for 3 h. In vivo results show that Psychrobacter sp. SE6 significantly decreases the feed conversion ratio (FCR) of E.coioides.Serum lysozyme activity shows no significant differences between the probiotic group and the control group during the whole experiment period, while an increasing trend of total superoxide dismutase (T-SOD) activity in the probiotic group is observed. Serum complement component 3 (C3) level of the probiotic group is higher than that of the control in the whole feeding period, while complement component 4 (C4) level of the probiotic group is significantly higher than that of the control at Day 60. The IgM level of probiotic group is significantly higher than that of control group at Day 30, but no significant difference is observed at Day 60. In conclusion, Psychrobacter sp. SE6 shows good antagonistic activities against potential pathogens and tolerance to mimic gastrointestinal environment, and the dietary administration of Psychrobacter sp. SE6 improves the feed utilization and immune responses of E.coioides.
- Psychrobacter sp.,
- Epinephelus coioides,
- antibacterial activity,
- growth performance,
- immunity

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References(26)

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