28S rDNA, ITS1 and adults morphological identification of <em>Neobenedenia</em> in cultured <em>Pseudosciaena crocea</em> at Xiangshan Harbor

MIAO Liang; LI Mingyun; JIANG Jin; DING Wenchao; CHEN Jiong; SHI Yuhong

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MIAO Liang, LI Mingyun, JIANG Jin, DING Wenchao, CHEN Jiong, SHI Yuhong. 28S rDNA, ITS1 and adults morphological identification of Neobenedenia in cultured Pseudosciaena crocea at Xiangshan Harbor[J]. Haiyang Xuebao, 2012, 34(2): 122-128.

Citation:

MIAO Liang, LI Mingyun, JIANG Jin, DING Wenchao, CHEN Jiong, SHI Yuhong. 28S rDNA, ITS1 and adults morphological identification of Neobenedenia in cultured Pseudosciaena crocea at Xiangshan Harbor[J]. Haiyang Xuebao, 2012, 34(2): 122-128.

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28S rDNA, ITS1 and adults morphological identification of Neobenedenia in cultured Pseudosciaena crocea at Xiangshan Harbor

Key Laboratory of Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo 315211 China

Received Date: 2011-03-30
Rev Recd Date: 2011-06-11

Abstract

Abstract

In recent years, Neobenedenia has caused serious damage on cage-cultured fish at Xiangshan Harbor, Zhejiang Province, China. The Neobenedenia adults (denoted by XSp) were collected from cultured Pseudosciaena crocea. The XSp sequences of 28S coding region of rRNA gene (28S rDNA) and the first internal transcribed spacer (ITS1) as well as morphology were studied for identification. The morphological characteristics showed that XSp belongs to Neobenedenia which was morphologically similar to Neobenedenia melleni. In XSp, the length of 28S rDNA was 393 bp and ITS1 was 427 bp. In phylogenetic analysis, Neobenedenia and Benedenia were split into two clades, and the XSp was in clade Neobenedenia. The sequences identities of 28S rDNA and ITS1 were 84.3%~89.5% and 60.2% among XSp and Benedenia, respectively. With one exception of 97.4%, the sequences identiteis of 28S rDNA and ITS1 among XSp, N. melleni and N. girellae were above 99%, which indicated that they are the same species. The results of two identification methods showed that the Neobenedenia at Xiangshan Harbor was N. melleni, and supported the opinion in which Whittington & Horton (1996) proposed that N. melleni and N. girellae were synonymy.
- Capsalidae,
- Neobenedenia melleni,
- morphological characteristics,
- 28S rDNA,
- ITS1,
- taxonomy

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References(21)

References

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