Molecular phylogeny of the crab genera <em>Helice</em> and <em>Helicana</em> based on the partial sequences of mitochondrial 16S rRNA gene

XU Jing-ming; SUN Han-chang; SUN Shi-chun

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XU Jing-ming, SUN Han-chang, SUN Shi-chun. Molecular phylogeny of the crab genera Helice and Helicana based on the partial sequences of mitochondrial 16S rRNA gene[J]. Haiyang Xuebao, 2011, 33(5): 124-132.

Citation:

XU Jing-ming, SUN Han-chang, SUN Shi-chun. Molecular phylogeny of the crab genera Helice and Helicana based on the partial sequences of mitochondrial 16S rRNA gene[J]. Haiyang Xuebao, 2011, 33(5): 124-132.

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Molecular phylogeny of the crab genera Helice and Helicana based on the partial sequences of mitochondrial 16S rRNA gene

1.
College of Life Science and Technology, Chongqing University of Arts and Sciences, Chongqing 402168, China
2.
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China

Received Date: 2009-04-02

Abstract

Abstract

The partial sequences of mitochondrial 16S rRNA gene of Helice tientsinensis (six populations), H. latimera (four populations), Helicana wuana (two populations) and H. japonica(one population) from the coast of China were determined and subjected to phylogenetic analysis with the inclusion of other species of Helice and Helicana obtained from the GenBank. The length of sequence of Helice tientsinensis and H. latimera was 525 bp, excluding 526 bp of two individuals from Dandong population of H. tientsinensis. Five haplotypes were found in them. The lengths of sequence of Helicana wuana and H. japonica were 525bp and 523bp respectively. There were no differences of sequence among individuals within populations of them, except Rizhao and Dandong populations. The A, T, G and C contents of them were similar, and the AT content(72.3%~73.7%)was higher than GC content. The resultant sequences of H. tientsinensis, H. latimera, H. wuana and H. japonica were 526bp long with 4 indels, of which 36 sites were variable and 26 sites were parsimoniously informative, and average transition/transversion ratio was 2.8. Among the five haplotypes, six individuals of H. latimera from Quanzhou and Ningbo populations and four individuals of H. tientsinensis from Ningbo and Rizhao populations shared one haplotype, and genetic distances among them were very small(0.19%~1.15%), implying that they may represent different morphotypes of the same species. Molecular phylogenetic trees indicated that the genera Helice and Helicana were monophyletic respectively, the large net genetic distances between them supported that they were updated from subgenus to genus. The molecular data also supported that the Pseudohelice and Parahelice were updated from subgenus to genus, and Helice/Chasmagnathus complex was divided into seven genera by morphology.
- Helice,
- Helicana,
- mitochondrial DNA,
- 16S ribosomal RNA gene,
- phylogeny

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References(23)

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