Construction and genetic analysis of gynogenetic clone and inbreeding strain of F<sub>1</sub> generation families for Japanese flounder<em> Paralichthys olivaceus</em>

TIAN Yong-sheng; WANG Di; WANG Lei; MIAO Gui-dong; DENG Han; CHEN Song-lin

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TIAN Yong-sheng, WANG Di, WANG Lei, MIAO Gui-dong, DENG Han, CHEN Song-lin. Construction and genetic analysis of gynogenetic clone and inbreeding strain of F1 generation families for Japanese flounder Paralichthys olivaceus[J]. Haiyang Xuebao, 2011, 33(3): 114-123.

Citation:

TIAN Yong-sheng, WANG Di, WANG Lei, MIAO Gui-dong, DENG Han, CHEN Song-lin. Construction and genetic analysis of gynogenetic clone and inbreeding strain of F₁ generation families for Japanese flounder Paralichthys olivaceus[J]. Haiyang Xuebao, 2011, 33(3): 114-123.

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Construction and genetic analysis of gynogenetic clone and inbreeding strain of F₁ generation families for Japanese flounder Paralichthys olivaceus

1.
Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao 266071,China
2.
Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao 266071,China;College of Life Sciences and Technology,Qingdao Agricultural University, Qingdao 266109, China
3.
Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao 266071,China;

Received Date: 2010-05-26

Abstract

Abstract

Three F₁ generation families of Japanese flounder Paralichthys olivaceus (0719, 0750 and 0751) with fast growth rate and high survival rate were screened out by selective breeding. In order to further purify these families'good traits, three inbreeding strains and seven gynogenetic clones induced by cryopreserved sperms of sea perch (Lateolabrax japoniaus) were constructed.The body lengths and masses of these two lines at 127 d and 190 d were analyzed by ANOVA, Student-Newman-Keuls and AGR. The results showed that 0751 gynogenetic clone grew fastest in all families (P ＜0.05). The chromosome ploidy analysis of lines from 0751 family revealed that inbreeding line and gynogenetic clone had the diploid characters with the similar DNA content at G₀-G₁ and G₂-M phases, as well the same DNA index at G₀-G₁ phase. Further, the genetic diversity detection of these strains by 14 pairs of microsatellite markers showed that the allelic number N_a was 2.214 3 and 2.000 0, the effective allelic number N_e was 2.082 9 and 1.936 9, the observed heterozygosity (H_o) was 0.564 2 and 0.413 8 (P＜0.05), the expected heterozygosity(H_e) was 0.5154 and 0.490 1, the average genetic heterozygosity(Ave_Het) was 0.5066 and 0.4828, and the polymorphism information content (PIC) was 0.403 and 0.366, respectively. Expect for three loci EKOP-E1-In,EKOP17-Li,EKOP-E1-Ey being highly polymorphic in the inbreeding line, others detected moderate polymorphism in both lines. Finally, the genetic deviation index(d) of the two lines was 0.091 5 and -0.157 3, suggesting that heterozygous excess was present in the inbreeding line, and heterozygosity deficiency and homozygous excess was present in the gynogenetic clone. This study shows that gynogenesis is a more effective method to promote gene purification and has great value in Japanese flounder breeding.
- Paralichthys olivaceus,
- inbreeding line,
- gynogenetic clone,

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References(33)

References

TABATA T. Studies on chromosome manipulation in hirame Paralichthys olivaceus[J]. Fish Genetics and Breeding Science, 1992, 13: 9-18.

YAMAMOTO S. Studies on sex manipulation and production of cloned populations in hirame, Paralichthys olivaceus (Temminck et Schlegel) [J]. Aquaculture, 1999, 173: 235-246.

PIFERRER F, CAL R, CASTORA G, et al. Induction of gynogenesis in the turbot (Scophthalmus maximus): effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age[J].Aquaculture, 2004, 238: 403-419.

LUCKENBACH A, GODWIN H, HARRY V, et al. Induction of diploid gynogenesis in southern flounder (Paralichthys lethostigma) with homologous and heterologous sperm[J]. Aquaculture, 2004, 237: 499-516.

KATO K, HAYASHI R, YUASA D, et al. Production of cloned red sea bream, Pagrosomus major, by chromosome manipulation[J]. Aquaculture, 2002, 207: 19-27.

FELIP A. The relationship between the effects of UV light and thermal shock on gametes and the viability of early developmental stages in a marine teleost fish, the sea bass(Dicent-rarchus labrax L.)[J]. Heredity, 1999, 83: 387-397.

CASTRO J, BOUZA C S, NCHEZ L, et al. Gynogenesis assessment using microsatellite genetic markers in turbot (Scophthalmus maximus)[J].Mar Biotechnol, 2003, 5(6): 584-592.

MORISHIMA K, NAKAYAMA I, ARAI K, et al. Microsatellite-centromere mapping in the loach, Misgurnus anguillicaudatus[J]. Genetics, 2001, 111: 59-69.

王晓清,王志勇,柳小春,等.大黄鱼人工诱导雌核发育后代的微卫星标记分析[J].遗传,2006,28(7):831-837.

吴清江,陈荣德,叶玉珍,等. 鲤鱼人工雌核发育及其作为建立近交系新途径的研究[J].遗传学报,1981,8(1):50-55.

张建森,孙小异. 建鲤新品系选育[J].水产学报,2007,31(3):287-293.

陈松林,田永胜,徐田军,等. 牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J].水产学报,2008,32(5):655-664.

田永胜,陈松林,徐田军,等. 牙鲆不同家系生长性能比较及优良亲本选择[J].水产学报,2009,33(6):901-912.

田永胜,徐田军,陈松林,等. 三个牙鲆育种群体亲本效应及遗传参数估计[J].海洋学报,2009,31(6):119-128.

JI X S, CHEN S L, YU G C, et al. Cryopreservation of sperm from sea perch (Lateolabrax japonicus) and feasibility for production-scale fertilization[J]. Aquaculture, 2004, 241: 517-528.

李思发,蔡完其.团头鲂双向选育效应研究[J].水产学报,2000,24(3): 201-206.

刘云国,陈松林,李八方,等.牙鲆选择性养殖群体遗传结构的微卫星分析[J]. 高技术通讯,2006,16(1):94-99.

KIM J H, KIM K K, LEE J H, et al. Isolation and characterization of polymorphic microsatellite loci in the olive flounder (Paralichthys olivaceus) [J]. Molecular Ecology Notes, 2003, 3: 491-493.

JI X S, CHEN S L, MA H Y, et al. Isolation and characterization of 19 EST-linked polymorphic microsatellite loci for olive flounder (Paralichthys olivaceus) [J]. Aquaculture Research, 2009, 40: 980-983.

KANG J H, KIM W J, LEE J H. Genetic linkage map of olive flounder, Paralichthys olivaceus[J]. International Journal of Biological Sciences, 2008, 4(3): 143-149.

ARAI K. Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan[J]. Aquaculture, 2001, 197: 205-228.

王忠卫,叶玉珍,周建峰,等.快速建立鲢、鳙纯系的初步研究[J].自然科学进展,2003,13(10):1104-1107.

吴清江,桂建芳.鱼类遗传育种工程[M].上海:上海科学技术出版社,1999.

郑春静,吴雄飞,刘东海,等. 用流式细胞仪检测大黄鱼三倍体[J].细胞生物学杂志,2006,28:253-256.

叶玉珍,周建峰,王忠卫,等. 三个鲫品系DNA含量的比较研究[J].水生生物学报,2004,28(1):13-17.

赵如榕,肖亚梅,刘少军,等. 雌核发育草鱼及亲本倍性研究[J].生命科学研究,2008,12(2):100-104.

JI X S, TIAN Y S, YANG J F, et al. Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers[J]. Aquaculture Research, 2010,41(6):913-920.

朱晓琛,刘海金,孙效文,等.微卫星评价牙鲆雌核发育二倍体纯合性[J].动物学研究,2006,27(1):63-67.

SEKINO M, HARA M. Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus [J]. Mar Biotechnol, 2001, 3: 310-315.

王伟,尤锋,高天翔,等.人工诱导牙鲆异质雌核发育群体的微卫星标记分析[J].高技术通讯,2005,15(7):107-111.

TANIGUCHI N, KIJIMA A, FUKAI J. High heterozygosity at Gpi-1 in gynogenetic diploids and triploids of ayu Plecoglossus altivalis [J]. Nippon Suisan Gakkaishi, 1987, 53 (5): 717-720.

徐成,王可玲,徐永立,等.雌核发育牙鲆同工酶基因的重组及父方基因的表达[J].海洋与湖沼,2002,33(1):62-67.

THORGAARD G H, ALLENDORF F W, KNUDSEN K L. Gene centromere mapping in rainbow trout: high interference over long map distances[J]. Genetics, 1983, 103: 771-783.

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