Screening and polymorphism of EST-microsatellite markers in <em>Litopenaeus vannamei</em>

ZHANG Qiong; LI Xi-lian; XUE Shu-wen; LIU Xiao-lin; HUANG Hao; XIANG Jian-hai

Article Contents

Article Navigation > Haiyang Xuebao > 2011 > 33(1): 121-126

ZHANG Qiong, LI Xi-lian, XUE Shu-wen, LIU Xiao-lin, HUANG Hao, XIANG Jian-hai. Screening and polymorphism of EST-microsatellite markers in Litopenaeus vannamei[J]. Haiyang Xuebao, 2011, 33(1): 121-126.

Citation:

ZHANG Qiong, LI Xi-lian, XUE Shu-wen, LIU Xiao-lin, HUANG Hao, XIANG Jian-hai. Screening and polymorphism of EST-microsatellite markers in Litopenaeus vannamei[J]. Haiyang Xuebao, 2011, 33(1): 121-126.

Citation:

PDF( 1020 KB)

Screening and polymorphism of EST-microsatellite markers in Litopenaeus vannamei

1.
Key Laboratory of Shaanxi Province for Molecular Biology for Agriculture, College of Animal Sciences and Technology, Northwest Agricalture & Forest University, Yangling 712100, China
2.
Nanjiang Marine Biotechnology Company Limited, Sanya 572000, China
3.
Open of Laboratory Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2010-03-04

Abstract

Abstract

Fourteen microsatellite DNA universal markers derived from Belgian wild shrimps expressed sequences tags (ESTs) were selected and evaluated. Stable banding pattern was shown by 7 EST-SSRs, and five of(CNM-MG 345, CNM-MG 390, CNM-MG 437, CNM-MG 444, CNM-MG 487) them were identified as the polymorphism EST-SSR, and subsequently used for genotyping of 240 cultured adult white shrimps sampled from Hainan Province. The characterization results, which revealed 2.400 0±0.547 7 alleles per locus, observed and expected heterozygosity levels ranged from 0.279 2 to 0.820 8 and from 0.377 9 to 0.601 1 respectively, showed the moderate polymorphisms at these loci. In addition, genetic difference between the Belgian wild population and Hainan cultured population was firstly found, which might caused by geographic isolation and selective breeding over the years. This study set The foundation for population genetics studies, function gene analysis and selective breeding was set in Hainan cultured Litopenaeus vannamei.
- Litopenaeus vannamei,
- EST-SSR,
- genetic diversity

FullText(HTML)

References(22)

References

ZHANG L, YANG C, ZHANG Y, et al. A genetic linkage map of Pacific white shrimp (Litopenaeus vannamei): sex-linked microsatellite markers and high recombination rates[J]. Genetica, 2007, 131(1): 37-49.

GORBACH D M, HU Z L, DU Z Q, et al. SNP discovery in Litopenaeus vannamei with a new computational pipeline[J].Animal Genetics, 2009, 40(1): 106-109.

CRUZ P, IBARRA A, MEJIA-RUIZ H. Genetic variability assessed by microsatellites in a breeding program of Pacific white shrimp(Litopenaeus vannamei)[J]. Marine Biotechnology, 2004, 6(2): 157-164.

VAN WORMHOUDT A, SELLOS D. Highly variable polymorphism of the alpha-amylase gene family in Litopenaeus vannamei(Crustacea Decapoda)[J]. J Molecular Evolution, 2003, 57(6): 659-671.

VALLES-JIMENEZ R, CRUZ P, PEREZ-ENRUQYEZ R. Population genetic structure of Pacific white shrimp(Litopenaeus vannamei) from Mexico to Panama: microsatellite DNA variation[J]. Marine Biotechnology, 2004, 6(5): 475-484.

MAGGIONI R, ROGERS A, MACLEAN N. Population structure of Litopenaeus schmitti(Decapoda : Penaeidae) from the Brazilian coast identified using six polymorphic microsatellite loci[J]. Molecular Ecology, 2003, 12(12): 3213-3217.

BLL A, CHAPMAN R. Population genetic analysis of white shrimp, Litopenaeus setiferus, using microsatellite genetic markers[J]. Molecular Ecology, 2003, 12(9): 2319-2330.

POWELL W, MACHRAY G C, PROVAN J. Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1996, 1(7): 215-222.

HU J, WANG X, HU X, et al. Existence of microsatellites in expressed sequence tags of common carp (Cyprinus carpio L.)available in GenBank dbEST Database[J]. Journal of Ocean University of China. 2006,5(1):12-20.

WANG M Q, HU J J, ZHUANG Y Y Z L, et al. In silico screening for microsatellite markers from expressed sequence tags of Porphyra yezoensis(Bangiales, Rhodophyta)[J]. Journal of Ocean University of China, 2007,6(2):161-166.

JI X S, CHEN S L, MA H Y, et al. Isolation and characterization of 19 EST-linked ploymorphic microsatellite loci for olive flounder(Paralichthys olivaceus)[J]. Aquaculture Research, 2009, 40(8): 980-983.

YU H, LI Q. Exploiting EST databases for the development and characterization of EST-SSRs in the Pacific oyster(Crassostrea gigas)[J]. Journal of Heredity. 2008, 99(2): 208-214.

佟广香,闫学春,匡友谊,等. 马氏珠母贝微卫星快速分离及遗传多样性分析[J]. 海洋学报, 2007, 29(4): 170-176.

PEREZ F, ORTIZ J, ZHINAULA M, et al. Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy[J]. Marine Biotechnoloy, 2005, 7(5): 554-569.

陈亮明,张冬林,李志辉,等. PAGE银染和条带回收方法的改进[J]. 中南林业科技大学学报:自然科学版,2007, 27(6): 163-165.

FRANKHAM R, BALLOU J, BRISCOE D. Introduction to Conservation Genetics[M].Cambridge: Cambridge University Press, 2002: 29-62.

RAYMAND M, ROUSSET F. GENEPOP (Version1.2): Population-genetics Software for Exact Tests and Ecumenicism[J]. J Hered 1995, 86(3), 248-249.

BOTSTEIN D, WHITE R, SKOLNICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hum Genet, 1980(32): 314-331.

CESAR J R, ZHAO B, YANG J. Analysis of expressed sequence tags from abdominal muscle cDNA library of the Pacific white shrimp Litopenaeus vannamei[J]. Animal, 2008, 2(9): 1377-1383.

GARCIA D K, ALCIVR-WARREN A. Characterization of 35 new microsatellite genetic markers for the Pacific whiteleg shrimp, Litopenaeus vannamei: their usefulness for studying genetic diversity of wild and cultured stocks, tracing pedigree in breeding programs, and linkage mapping[J]. Journal of Shellfish Research, 2007, 26(4): 1203-1216.

WANG X, GUO X, ZHANG Y, et al. Development of polymorphic EST-derived SSR markers for the shrimp, Fenneropenaeus chinensis[J]. Conservation Genetics, 2009, 10: 1455-1457.

王鸿霞,吴长功,张留所,等. 微卫星标记应用于凡纳滨对虾家系鉴别的研究[J]. 遗传, 2006, 28(2):179.

Relative Articles

Supplements(0)

Cited By

Proportional views