Characteristics of biofilm formation of pathogenic <em>Vibrio parahaemolyticus</em>

CHEN Zhen; QIN Ying-xue; ZOU Wen-zheng; XU Xiao-jin; XING Yan-li; YAN Qing-pi

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CHEN Zhen, QIN Ying-xue, ZOU Wen-zheng, XU Xiao-jin, XING Yan-li, YAN Qing-pi. Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus[J]. Haiyang Xuebao, 2010, 32(5): 110-116.

Citation:

CHEN Zhen, QIN Ying-xue, ZOU Wen-zheng, XU Xiao-jin, XING Yan-li, YAN Qing-pi. Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus[J]. Haiyang Xuebao, 2010, 32(5): 110-116.

CHEN Zhen, QIN Ying-xue, ZOU Wen-zheng, XU Xiao-jin, XING Yan-li, YAN Qing-pi. Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus[J]. Haiyang Xuebao, 2010, 32(5): 110-116.

Citation:

CHEN Zhen, QIN Ying-xue, ZOU Wen-zheng, XU Xiao-jin, XING Yan-li, YAN Qing-pi. Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus[J]. Haiyang Xuebao, 2010, 32(5): 110-116.

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Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus

Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China

Received Date: 2010-01-17

Abstract

Abstract

Biofilm formation of 12 strains of Vibrio were determinated using the method of modified microtiter-plate test,and V. parahaemolyticus strain ND-02 was chosen for the further study on the influence of different environmental factors on bacterial biofilm formation .The results show that V. parahaemolyticus strain ND-02 forms mature biofilm in 24～36 h,V. parahaemolyticus with original bacterial concentrations of 10⁷ and 10⁸CFU/mL shows the largest biofilm quantity; the OD₅₉₀ value of bacterial biofilm increases with the incubation temperature from 10 ℃ to 30 ℃ and peaks at 30 ℃;the OD₅₉₀ value of bacterial biofilm fluctuates with different NaCl concentrations optimized at 3% to 5%,and OD₅₉₀ values are remarkably higher at pH 7～10,and peak at pH 7.Addition of Ca²⁺ obviously promoted the bacterial biofilm formation while Mg²⁺ played as an opposite role. V. parahaemolyticus strain ND-02 showed better biofilm formation on the wells coated with liver extract,skin mucus and intestinal mucus of Pseudosciaena crocea,the less biofilm was found on the wells coated with muscle extract. The results indicate that V. parahaemolyticus strain ND-02 develops stable and evident biofilm,the biofilm formation is remarkably affected by environmental factors, such as temperature, pH, NaCl concentration,Ca²⁺ ,Mg²⁺ . The results also indicate that the bacterial biofilm formation of V. parahaemolyticus is governable,which will be useful for the epidemic control in fish culture.
- Vibrio parahaemolyticus,
- biofilm,
- environmental factors

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References(31)

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