A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF

ZHENG Rong-hui; ZHANG Yu-sheng; CHEN Qing-fu

Article Contents

Article Navigation > Haiyang Xuebao > 2010 > 32(5): 60-66

ZHENG Rong-hui, ZHANG Yu-sheng, CHEN Qing-fu. A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF[J]. Haiyang Xuebao, 2010, 32(5): 60-66.

Citation:

ZHENG Rong-hui, ZHANG Yu-sheng, CHEN Qing-fu. A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF[J]. Haiyang Xuebao, 2010, 32(5): 60-66.

ZHENG Rong-hui, ZHANG Yu-sheng, CHEN Qing-fu. A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF[J]. Haiyang Xuebao, 2010, 32(5): 60-66.

Citation:

ZHENG Rong-hui, ZHANG Yu-sheng, CHEN Qing-fu. A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF[J]. Haiyang Xuebao, 2010, 32(5): 60-66.

PDF( 1125 KB)

A comparison of the hepatic microsomal EROD activity in three species of marine fish exposed to crude oil WSF

Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2010-04-10

Abstract

Abstract

The dose response, time-course induction and rehabilitation of hepatic microsomal EROD activity from Sparus macrocephalus、Sparus latus and Sebastiscus marmoratus were studied by crude oil water soluble fraction (WSF). The results show that in the dose-response experiments for 5 d, the hepatic EROD activity is significantly induced at the dose of 50 μg/dm³ WSF for Sebastiscus marmoratus and at the dose of 75 μg/dm³ WSF for both Sparus macrocephalus and Sparus latus; the induction of the hepatic EROD activity is the highest in Sebastiscus marmoratus, but has a wider concentration range in Sparus macrocephalus. In the time-course experiments exposure to 40 μg/dm³ WSF, the hepatic EROD activity of Sparus latus is significantly induced after 2 d of exposure; three species of fish hepatic EROD activities are significantly induced and reach a maximal level after the exposure of 4 d, then decreased and close to the control levels after 6 d of exposure. In the rehabilitation experiment, three species of fish hepatic EROD activities decrease and return to the control levels after 4 or 8 d of exposure. In general, the hepatic EROD activity from the above three species of fish can all be used for monitoring the oil pollution; However, for the comparison of the three species of fish, Sebastiscus marmoratus is more sensitive to the crude oil WSF and more suitable for monitoring marine environmental oil pollution and its biochemical effect, especially low-dose biochemical effect.
- Sparus macrocephalus,
- Sparus latus,
- Sebastiscus marmoratus,
- hepatic microsomal EROD,
- crude oil WSF,
- biochemical effects

FullText(HTML)

References(21)

References

冯士筰, 王辉. 海洋科学[M]. 北京: 海洋出版社, 2001.

张玉生. 加快海洋污染生物效应监测新技术研究保护厦门海域生态环境 //厦门市海洋经济发展战略和海洋环境保护研讨会论文集.北京: 海洋出版社, 2006: 104—107.

BALK L.Characterization of xenobiotic metabolism in the feral teleost northern pike (Esox lucius) .Stockholm:Stockholm University,1985.

GB 17378.4-2007 海洋监测规范:第4部分:海水分析[S].北京:中国标准出版社,2007.

GB 3097—1997 海水水质标准[S].北京:国家环境保护局,1997.

余铭恩.褐菖鲉(Sebastiscus marmoratus)肝微粒体细胞色素P-450加单氧酶的研究——动力学反应条件、诱导和监测 .厦门:国家海洋局第三海洋研究所, 2003.

STEGEMAN J J, BINDER R.L, ORREN A. Hepatic and extrahepatic microsomal election transport components and mixed-function oxygenases in the marine fish stenotomus versioolor[J]. Biochem Pharmacol, 1979, 25: 3431—3439.

FORLIN L. Effect of clophen A50, 3-methylcholanthrene, pregnenolone-16α carbonitrile and phenobarbital on the hepatic microsomal cytochrome P-450-dependent monooxygenase system in rainbow trout(Salmo garidneri), of different age and sex[J]. Toxicol Appl Pharmacol, 1980, 54: 420—430.

BURKE M D, MAYER R T. Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene[J]. Drug Metab Dispos, 1974, 2: 583—588.

郭敏亮, 姜涌明. 考马斯亮蓝显色液组分对蛋白质测定的影响[J]. 生物化学与生物物理进展, 1996, 23(6): 558—561.

贾小平, 林钦, 吕晓瑜,等. 广东沿海牡蛎石油烃污染研究[J]. 海洋环境科学, 1990, 9(1): 13—17.

RICHARD T, GIULIO D, HABIG C, et al. Effects of Black Rock Harbor sediments on indices of biotransformation, oxidative stress, and DNA intergrity in channel catfish [J]. Aquat Toxicol, 1993, 26:1—22.

AAS E, KLUNGSOR J. PAH metabolites in bile and EROD activity in North sea fish [J]. Marine Environmental Research, 1998, 46(1-5): 229—232.

BILLIARD S M, BOLS N C, HODSON P V. In vitro and in vivo comparisons of fish specific CYP1A induction relative potency factorsfor selected polycyclic aromatic hydrocarbons[J]. Ecotoxicology and Environmental Safety, 2004, 59: 292—299.

BOSVELD A T, DE BIE PA, VAN DEN BRINK N W, et al. In vitro EROD induction equivalency factors for the 10 PAHs generally monitored in risk assessment studies in The Netherlands[J]. Chemosphere, 2002, 49: 75—83.

FENT K., BTSCHER R. Cytochrome P450 1A induction potencies of polycyclic aromatic hydrocarbons in a fish hepatoma cell line: demonstration of additive interactions.Environ[J]. Toxicol Chem, 2000, 19: 2047—2058.

陈加平,徐立红,吴振斌,等. 苯并(a)芘致毒的鱼的分子生态毒理学指标研究[J]. 环境科学, 1999, 19(5): 417—420.

WANG Yun, ZHENG Rong-hui, ZUO Zheng-hong, et al. Relation of hepatic EROD activity and cytochrome P4501A level in Sebastiscus marmoratus exposed to benzo pyrene[J]. Journal of Environmental Sciences, 2008, 20(1): 101—104.

PACHECO M, SANTOS M A. Biotransformation, endocrine, and genetic responses of Anguilla anguilla L.to petroleum distillate products and environmentally contaminated waters[J]. Ecotoxicology and Environmental Safety, 2001, 49: 64—75.

ROBERT E, MARK G, STANLEY D, et al. Mixed function oxygenase induction in pre- and post-spawn Herring(Clupea pallasi)by petroleum hydrocarbons[J]. Comp. Biochem Physiol, 1997, 166C(2): 141—147.

LIVINGSTONE D R, ARCHIBALD S, CHIPMAN J K, et al. Antioxidant enzymes in liver of the dab (Limanda-limanda)from the North Sea[J].Mar Lcol Prog Ser, 1992, 91: 97—104.

Relative Articles

Supplements(0)

Cited By

Proportional views