Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea

ZHANG Jian; ZHAO Yang-guo; LI Hai-yan; BAI Jie; TIAN Wei-jun

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ZHANG Jian, ZHAO Yang-guo, LI Hai-yan, BAI Jie, TIAN Wei-jun. Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea[J]. Haiyang Xuebao, 2010, 32(2): 118-127.

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Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea

Key Laboratory of Ministry of Education for Marine Environment and Ecology, Ocean University of China, Qingdao 266100, China

Received Date: 2009-07-05
Rev Recd Date: 2010-02-02

Abstract

Abstract

16S rDNA clone library combining with denaturing gradient gel electrophoresis (DGGE) technique was used to reveal the bacterial community diversity and the spatial distribution characteristics from the three stations of the sediment of the northwest Huanghai Sea. The bacterial 16S rDNA libraries of the surface sediments showed that at all three stations composed high diversity of bacterial communities, γ-and δ-proteobacteria were the donminant groups, respectively, accounting for 20% and 32% of the total sequences. DGGE profiles showed that the bacterial community structure presented a high degree of similarity at the same stations at different depths, but demonstrated far distance from different stations. The present study shows that the bacteria from the coastal sediments have a high community diversity and possess dominant groups in bacterial community, but on a smaller scale, the vertical changes of community structure are not obvious.
- 16S rDNA clone library,
- denaturing gradient gel electrophoresis (DGGE),
- bacterial community

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