The spermatogensis and male gonadal histology of Cellana grata(Gould)

The spermatogenesis and male gonadal histology of Cellana grata (Gould) along the coast of west Guangdong were observed with historical section. The spermatogenesis of this shell goes through stages of proliferation, growth, maturation and metamorphism. According to the morphological transformation of seminiferous cell, the process of spermatogenesis could be divided into five stages, spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and spermatozoon. The male gonad was composed of follicle and the sperm in the follicle cavity were expelled from efferent duct. The result from observing and analyzing shows that; (l)the stage of growth was not obvious during spermatogenesis while the primary spermatocyte were smaller than the spermatogonium, (2) the seminiferous cell and its nuclei transformed smaller during the spermatogenesis and the basophilic characteristic of the nuclei was gradually intensified, (3) The diversiform spermatid was easily observed during spermatogenesis duo to the longer metamorphism stage, (4) mature sperm was 0.5×3~4 μm, with bacilliform head and flagellum tail,(5) the male gonad at growth and maturity stage was made of regular(polygon) follicle with explicit circumscription, (6)the male follicle wall was made up of seminiferous cell of different stage arranged in file,the primal seminiferous cells, which were acidophilic, was closer to the follicle velum,the termal seminiferous cells which were basophilic, were apart from the follicle velum, and the spermatozoa show swirl in the antrum, (7)the mature male gonad can embed into the tissue of foot and liver. Four structures of germ efferent duct at different levels can be observed in liver tissue section, (8)Cellana grata (Gould) was dioecism,meanwhile the phenomena of hermaphrodite and sex reversal were observed.