ISSR analysis of wild and farmed stone flounder(<i>Kareius bicoloratus</i>) stocks

XU Jian-peng; ZHANG Quan-qi; WANG Zhi-gang; QI Jie; WANG Xu-bo

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XU Jian-peng, ZHANG Quan-qi, WANG Zhi-gang, QI Jie, WANG Xu-bo. ISSR analysis of wild and farmed stone flounder(Kareius bicoloratus) stocks[J]. Haiyang Xuebao, 2009, 31(4): 128-134.

Citation:

XU Jian-peng, ZHANG Quan-qi, WANG Zhi-gang, QI Jie, WANG Xu-bo. ISSR analysis of wild and farmed stone flounder(Kareius bicoloratus) stocks[J]. Haiyang Xuebao, 2009, 31(4): 128-134.

XU Jian-peng, ZHANG Quan-qi, WANG Zhi-gang, QI Jie, WANG Xu-bo. ISSR analysis of wild and farmed stone flounder(Kareius bicoloratus) stocks[J]. Haiyang Xuebao, 2009, 31(4): 128-134.

Citation:

XU Jian-peng, ZHANG Quan-qi, WANG Zhi-gang, QI Jie, WANG Xu-bo. ISSR analysis of wild and farmed stone flounder(Kareius bicoloratus) stocks[J]. Haiyang Xuebao, 2009, 31(4): 128-134.

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ISSR analysis of wild and farmed stone flounder(Kareius bicoloratus) stocks

1.
Key Laboratory of Ministry of Education, for Marine Genetics and Gene Resource Exploitation, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;College of Life Science, Ludong University, Yantai 264025, China
2.
Key Laboratory of Ministry of Education, for Marine Genetics and Gene Resource Exploitation, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

Received Date: 2008-11-18
Rev Recd Date: 2009-02-26

Abstract

Abstract

Inter-simple sequence repeat(ISSR) was employed to detect the genetic diversity in three wild and one farmed stocks of stone flounder(Kareius bicoloratus).Of the 60 primers screened,eight were selected for their reproducibility,reliability and high poly morphism.Atotal of 183 loci were am plified from the investigated individuals of the 4 stocks.The percentage of polymorphic lociranged from 48.1% to 50.8% and the Shannon diversity indices were from 15.68 to 18.53 within the 4 stocks.The genetic similarities were 0.9053~0.9189 within stocks,and the genetic distances were 0.0055~0.0173 among stocks.Compared with other fish species,stocks of K.bicoloratus presented relatively low genetic diversity.The phylogenetic tree constructed through UPGMA method indicated that WH and LZ stocks were first clustered into one clade for the lowest genetic distance,and then clustered with YZ stock,at last with QD stock.The analysis of stock pairwise gene differentiation showed that 96.47% of the genetic variation was distributed within stocks,while only 3.53% among stocks,indicating that the genetic structures of different stocks were absolutely similar.
- stone flounder,
- Kareius bicoloratus,
- ISSR

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References(23)

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