The application of microsatellite markers for genealogical identification in selective breeding program of turbot(<i>Scophthalmus maximus</i> L.)

YU Fei; WANG Wei-ji; KONG Jie; RUAN Xiao-hong

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Article Navigation > Haiyang Xuebao > 2009 > 31(3): 127-136

YU Fei, WANG Wei-ji, KONG Jie, RUAN Xiao-hong. The application of microsatellite markers for genealogical identification in selective breeding program of turbot(Scophthalmus maximus L.)[J]. Haiyang Xuebao, 2009, 31(3): 127-136.

Citation:

YU Fei, WANG Wei-ji, KONG Jie, RUAN Xiao-hong. The application of microsatellite markers for genealogical identification in selective breeding program of turbot(Scophthalmus maximus L.)[J]. Haiyang Xuebao, 2009, 31(3): 127-136.

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The application of microsatellite markers for genealogical identification in selective breeding program of turbot(Scophthalmus maximus L.)

1.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200090, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China
3.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China

Received Date: 2008-12-05
Rev Recd Date: 2009-05-28

Abstract

Abstract

Eight microsatellite markers were used to estimate the kinship and the genetic diversity of seven turbot(Scophthalmus maximus L.) families. The average polymorphic information content(PIC) and the average heterozy gosity were 0.6721 and 0.7606,respectively. To tally forty alleles were detected at eight microsatellite loci in seven full and hal-fsib families of turbot. The number of the alleles at each locus ranged from three to eight. Based on the genotypes of either known parentor none of them and of fspring, all the absent parental genotypes of the seven families were deduced. According to the uniquealleles among the families,each family could be disting uished from the others. Cervus 3.0 analysis showed that the combined exclusion probability was 97.07% if parents were both unknown and the combined exclusion probability was 99.63% when only one of the parental geno type was known. A dendrogram was constructed using UPGMA based on genetic similarity matrix. The UPGAM dendrogram of the 137 individuals indicated that 90.5% of the individuals from each family could befully clustered and the classification results accorded with the source of genealogy. The results showed that microsatellite marker is useful tool for genealogical identification of turbot.
- microsatellites,
- Scophthalmus maximus L.,
- genealogical identification

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References(23)

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