Hemolytic activity of <I>Karlodinium micrum</I> at different growth phases and analysis of hemolysin species

ZHOU Cheng-xu; FU Yong-jing; CHEN Qing-fieng; YAN Xiao-jun

Article Contents

Article Navigation > Haiyang Xuebao > 2009 > 31(2): 146-151

ZHOU Cheng-xu, FU Yong-jing, CHEN Qing-fieng, YAN Xiao-jun. Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species[J]. Haiyang Xuebao, 2009, 31(2): 146-151.

Citation:

ZHOU Cheng-xu, FU Yong-jing, CHEN Qing-fieng, YAN Xiao-jun. Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species[J]. Haiyang Xuebao, 2009, 31(2): 146-151.

Citation:

PDF( 514 KB)

Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species

Ningbo University, Key Laboratory of Marine Biotechnology, Ningbo 315211, China

Received Date: 2008-12-15
Rev Recd Date: 2009-01-10

Abstract

Abstract

Hemolytic activity of the cell lysate and culture medium of Karlodinium micrum were tested at various algal growth phases, as well as to compare the difference between ethyl acetate and Bligh-Dyer, the major components of the hemolysin were fractionated with column chromatography and thin-layer chromatography.The results showed that the hemolytic activity in cells of K.micrum at the four growth phases maintained at a high level, whereas high hemolytic activity was only observed in the stationary and senescent phases in the filtrates the culture media, indicating that the hemolysin of K.micrum are normal physiological metabolites, and can be released into the environment when cells are in aging process.Ethyl acetate extraction and Bligh-Dyer extraction are both feasible to extract the hem olysin.Through chromatographic separation and hemolytic activity assay tracking, we found that there were three major hemolysin fractions in K.micrum, and two polyunsaturated fatty acid, namely 18:5n3 and 22:6n3, may play an important role in hemolysis.
- Karlodinium micrum,
- hemolysin,
- growth cycle

FullText(HTML)

References(17)

References

周成旭,傅永静,严小军.新型赤潮种类微小卡罗藻的溶血毒性检测[J].海洋通报,2007,26(3):112-116.

BLANKENSHlP B.Scientists isolate toxin from fish-killing algae in watershed[OL].Bay Journal,2002-10.[2008-03-27].http:/www.bayjournal.com/article.cfm?article=870.

KEMPTON J W,.LEWITUS A J.DEEDS J R,et al.Toxicity of Karlodinium micrum(Dinophyceae)associated with a fish kill in a South Carolina brackish retention pond[J].Harmful Algae,2002,1(1):233-241.

GLIBERT P M,MAGNIEN R,LOMAS M W,et al.Harmful algal blooms in the Chesapeake and coastal bays of Maryland[J].Estuaries,2001,24:875-883.

DEEDS J R,TERLIZZI D E,ADOLF J E,et al.Toxic activity from cultures of Karlodinium micrum(=Gyrodinium galatheanum)(Dinophyecae)-a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility[J].Harmful Algae,2002,1(1):169-189.

JACKIE Y C,LIU J H.Marine water quality in Hongkong in 2004[R].2005:8-16.

周成旭,严小军.组织培养板在获取微藻种质中的应用[J].海洋技术,2006,25(4):5457.

AKIKO E,YUKIHIKO M,TATSUYA O.Evidence for the production ot a novel proteinaceous hemolytic exotoxin by dinoflagellate Alexandrium taylori[J].Harmful Algae,2004(3):29-37.

彭喜春,彭志英,等.实验室培养球形棕囊藻溶血毒素的提取、分离及其生成特征[J].热带亚热带植物学报,2005,13(1):25-28.

HALLEGRAEFF G M.Harmful algal blooms in the Australian region[J].Marine Pollution Bulletin,1992,25(5):186-190.

PLACE A,DEEDS J R.Dinoflagellate karlotoxins,methods of isolation and uses thereof:US,100659[P].2004-11-25.

BLIGH E G,DYER W J.A rapid method lipid extraction and purification[J].Can J Biochem Physiol,1959,37:911-923.

徐继林,严小军,周成旭,等.一种海洋席藻淡化过程中脂肪酸组成的应急变化[J].水生生物学报,2004,28(4):374-379.

彭喜春,刘洁生,杨维东.赤潮藻毒素生物合成研究进展[J].热带亚热带植物学报,2006,14(1):81-86.

YASUMOTO T,UNDERDAL B,AUNE T,et al.Screening for hemolytic and ichthyotoxic compounds of Chrysochromulina polylepis and Gyrodinium aureohum from Norwegian coastal waters[J].Toxic Marine Phytoplankton,1990,46:436-440.

YASUMOTO T,SENIMO N,MURAKAMI Y.Toxins Produced by Benthic Dinoflagellates[J].Biol Bull,1987,172:128-131.

PARRISH CC,BODENNC G,GENTIEN P.Haemolytic Glycoglycerolipids from Gymnodinium Species[J].Phytochem,1998,47(5):783-787.

Relative Articles

Supplements(0)

Cited By

Proportional views