The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of <I>Portunus trituberculatus</I> in East China Sea

YAO Gui-gui; WU Xu-gan; CHENG Yong-xu; YANG Xiao-zhen; WANG Chun-lin

Article Contents

Article Navigation > Haiyang Xuebao > 2008 > 30(6): 122-131

YAO Gui-gui, WU Xu-gan, CHENG Yong-xu, YANG Xiao-zhen, WANG Chun-lin. The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea[J]. Haiyang Xuebao, 2008, 30(6): 122-131.

Citation:

YAO Gui-gui, WU Xu-gan, CHENG Yong-xu, YANG Xiao-zhen, WANG Chun-lin. The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea[J]. Haiyang Xuebao, 2008, 30(6): 122-131.

Citation:

YAO Gui-gui, WU Xu-gan, CHENG Yong-xu, YANG Xiao-zhen, WANG Chun-lin. The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea[J]. Haiyang Xuebao, 2008, 30(6): 122-131.

PDF( 1372 KB)

The changes of histology and main biochemical composition in the hepatopancreas at the different physiological stages of Portunus trituberculatus in East China Sea

1.
Institute of Shanghai High Education of Aquaculture, Shanghai Ocean University, Shanghai 200090, China;Institute of Fisheries, Hangzhou Academy of Agriculture Science, Hangzhou 310024, China
2.
Institute of Shanghai High Education of Aquaculture, Shanghai Ocean University, Shanghai 200090, China
3.
College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China

Received Date: 2007-12-24
Rev Recd Date: 2008-07-24

Abstract

Abstract

The study was conducted to investigate the changes in the hepatosomatic index (HSI), the main biochemical compositions and its histological feature of Portunustrituberculatus at the different physiological stages in East Sea by biochemical determination and hist olog ical examination.Furthermore, the relationship between changing histology and main biochemical composition in the hepat opancreas were also discussed in the end of this paper.The results showed that: (1) During the ovarian maturation, the HSI increased to (7.46±1.23)% at stage Ⅳ, then decreased to (4.62±0.63)% at stage Ⅳ; (2) Although the stable protein level (10%~12% wet weight) could be found in wet hepatopancreas during the ovarian development, there were significant differences in the other main biochemical compositions during the course of ovarian maturation.The highest water content of the hepato pancreas could be found at the stage Ⅰ, which reached 67.91%.Then, the total lipid (TL) content based on wet hepat opancreas appeared as the trend of "low—high—low".The peak TL content (24.24% wet weight) was found at stage Ⅳ in wet hepatopancreas, while the lowest TL content was only 13.48% at stage.Moreover, the carbohydrate content of wet hepat opancreas decreased slightly except for stage.(3) The significant changes of hepatopancreatic histology happened in the reproductive molting and spawning stages in female crabs.In early stage Ⅰ (before reproductive molting), B cells dominated in the hepat opancreatic tubules while R cells seemed slender.Meanwhile, the abundant food residue and vacuoles sloughed off by B cells were found in tubule lumens of the hepatopancreas.In stage Ⅱ (after reproductive molting), the numbers of R cells increased significantly, which were filled with lipid droplets with ovarian development.After spawning, the boundary of many hepat opancreatic cells became unclear or even mixed.However, there were more B and F cells on the hepat opancreas than before, which suggested that crabs restart feeding to ensure the second ovarian development as the water temperature increased.
- Portunustrituberculatus,
- hepatopancreas,
- different physiological stages,
- biochemical composition,
- histological feature

FullText(HTML)

References(42)

References

MIKAMI S,GREENWOOD J G.Functional morphology and cytology of the phyllosomal digestive system of Sivacus ciliatus and Panulirus japonicus (Decapoda)[J].Crustaceana,1994,67:212-225.

成永旭,堵南山,赖伟.不同阶段中华绒螯蟹肝胰腺的脂类及脂肪酸组成[J].动物学报,1998,44(4):420-429.

成永旭,李少菁,王桂忠.锯缘青蟹幼体肝胰腺细胞结构变化与其营养状况的关系:Ⅰ.溞状幼体期Ⅰ期的研究[J].厦门大学学报(自然科学版),1998,37(4):576-581.

成永旭,堵南山,赖伟.中华绒螯蟹肝胰腺R和F细胞及其脂类贮存的电镜研究[J].动物学报,2000,46(1):8-13.

李富花,李少菁.锯缘青蟹幼体肝胰腺的观察研究[J].海洋与湖沼,1998,29(1):29-34.

ROSA R,NUMES M L.Biochemical changes during the reproductive cycle of the deep-sea decapod Nephrops norvegicus on the south coast of Portugal[J].Marine Biology,2002,141:1001-1009.

VOGT G,STOCKER W,STORCH V,et al.Biosynthesis of Astacus protease,a digestive enzyme from crayfish.[J].Histochemistry,1989,91:373-381.

VOGT G.Life-cycle and functional cytology of the hepatopancreatic cells of Astacus astacus (Crustacea,Decapoda)[J].Zoomorphology,1994,114:83-101.

VOGT G,STORCH V,QUINITIO E T,et al.Midgut gland as monitor organ for the nutritional value of diets in Penaeus monodon(Decapoda)[J].Aquaculture,1985,48:1-12.

MOURENTE G,MEDINA A,GONZATEZ S,et al.Changes in lipid class and fatty acid contents in the ovary and midgut gland of the female fiddle crab Uca tangeri(Decapoda,Ocypodiadae) during maturation[J].Marine Biology,1994,121:187-197.

李太武.三疣梭子蟹肝脏的结构研究[J].海洋与湖沼,1996.27(5):471-477.

谢忠明,刘洪军,冯蕾.海水经济蟹类养殖技术[M].北京:中国农业出版社,2002:15-27.

李太武,苏秀榕.三疣梭子蟹消化系统的组织学研究[J].辽宁师范大学学报(自然科学版),1994,17(3):230-237.

朱丽岩,郑家声,王梅林,等.三疣梭子蟹幼体中肠腺发育的组织学和细胞学研究[J].青岛海洋大学学报,2000,30(4):631-636.

吴旭干,姚桂桂,杨筱珍,等.东海三疣梭子蟹卵巢发育规律的研究[J].海洋学报,2007,29(4):120-128.

WILLIAMS S.Official Methods of Analysis of the Association of Official Analytical Chemists.14th[M].Washington:Science Press,1984:1018.

FOLCH J,LEES M,STANLEY G H S.A simple method for the isolation and purification of total lipids from animal tissues[J].Journal of Biological Chemistry,1957,226:497-509

HOLLAND D L,CABBOTT P A.A micro-analytical scheme for the determination of protein,carbohydrate,lipid and RNA levels in marine invertebrate larvae[J].Journal of Marine Biology Association of UK,1971,51:659-668.

张宝琳,相建海.长江口海区三疣梭子蟹和细点圆趾蟹食性生态因子的研究[J].海洋科学,1991,5:60-64.

PILLAY K K,NAIR N B.Observations on the biochemical changes in the gonads and other organs of Uca annulipes,Portunus pelagicus,and Metapenaeus affinis during the reproductive cycle[J].Marine Biology,1973,18:167-198.

WOUTERS R,MOLINA C,LAVENS P,et al.Lipid composition and vitamin content of wild female Litopenaeus vannamei in different stages of sexual maturation[J].Aquaculture,2001,198:307-323.

CAVALLI R O,MONTAKAN T,LAVENS P,et al.Variation in lipid classes and fatty acid content in tissues of wild Macrobrachium rosenbergii(deMan) females during maturation[J].Aquaculture,2001,193:311-324.

于志勇,吴旭干,成永旭,等.中华绒螯蟹第二次卵巢发育过程中肝胰腺和卵巢中主要生化成分的变化[J].水生生物学报,2007,31(6):45-52.

HEATH J R,BARME S.Some changes in biochemical composition with season and during the molt cycle of the common shore crab,Carcinus maenas[J].Journal of Experimental Marine Biology and Ecology,1970,5:199-233.

张文军,贺诗水,成永旭,等.蜕壳前后中华绒螯蟹幼蟹肝胰腺脂肪酸组成的变化[J].上海师范大学学报(自然科学版),2002,31(增刊):24-27.

JACOBS W.Untersuchungen uber die cytologle der Sekretbildung in der Mitteldarmdruse von Astacus leptodactylus[J].Cell and Tissue Research,1928,8:1-62.

AL-MOHANNA S Y,NOTT J A,LANE D J W.M-midget'cells in the hepatopancreas of the shrimp,Penaeus semisulcatus De Haan,1844(Decapoda,Natantia)[J].Crustaceans,1985,48(3):260-268.

AL-MOHANNA S Y,NOTT J A,LANE D J W.Mitotic E-and secretory F-cell in the hepatopancreas of the shrimp Penaeus semisulcatus (Crustacea:Decapoda)[J].Journal of Marine Biology Association of UK,1985,65:901-910.

AL-MOHANNA S Y,NOTT J A.B-cells and digestion in the hepatopancreas of Penaeus semisulcatus(Crustacea:Decapoda)[J].Journal of Marine Biology Association of UK,1986,66:403-414.

DAVIS L D,BURNETT A L.A study of growth and cell differentiation in the hepatopancreas of the crayfish[J].Development Biology,1964,10:122-153.

STANIER J E,WOODHOUSE M A,GRIFFIN R L.The fine structure of the hepatopancreas of Carcinus maenas (L.) (Decapoda:Brachyura)[J].Crustaceans,1968,14:56-66.

LOIZZI R F.Interpretation of crayfish hepatopancreatic function based on fine structural analysis of epithelial cell lines and muscle network[J].Z Zellforsch,1971,113:420-440.

LOIZZI R F,PETERSON D R.Lipolytic sites in crayfish hepatopancreas and correlation with fine structure[J].Comparative Biochemistry and Physiology,1971,39B:227-236.

HOPKIN S.P,NOTT J A.Some observations on concentrically structured,intracellular granules in the hepatopancreas of the shore crab Carcinus Maenas(L.)[J].Journal of Marine Biology Association of UK,1979,59:867-877.

HOPKIN S P,NOTT J A.Studies on the digestive cycle of the shore crab Carcinus maenas(L.) with special reference to the B cells in the hepatopancreas[J].Journal of Marine Biology Association of UK,1980,60:891-907.

CACECI T,NECK K F,LEWIS D H,et al.Ultrastructure of the hepatopancreas of the pacific white shrimp,Penaeus vannamei (Crustacea:decapoda)[J].Journal of Marine Biology Association of UK,1988,68:323-337.

朱丽岩,郑家声,王梅林,等.三疣梭子蟹幼体中肠腺发育的组织学和细胞学研究[J].青岛海洋大学学报,2000,30(4):631-636.

OGURA K.Midgut gland cells accumulating iron or copper in the crayfish,Procambarus clarkii[J].Annot Zool Japon,1959,32:133-142.

姚桂桂,吴旭干,杨筱珍,等.三疣梭子蟹卵巢的第二次发育特征[J].动物学研究,2007,29(4):423-429.

LI K,CHEN L Q,LI E C,et al.The site of vitellogenin synthesis in Chinese mitten-handed crab Eriocheir sinensis[J].Comparative Biochemistry and Physiology,2006,143B:453-458.

MAK A S,CHOI C L,TSUI S H,et al.Vitellogenesis in the red crab Charybdis feriatus:hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression[J].Molecular Reproduction and Development,2005,70:288-300.

CHAN S M,MAK A S,TSUL S H,et al.Vitellogenesis in the red crab,Charybdis feriatus:Contributions from small vitellogenin transcripts (CfVg) and farnesoic acid stimulation of CfVg expression[J].Annals New York Academy Science,2005,1040:74-79.

Relative Articles

Supplements(0)

Cited By

Proportional views