Molecular identification of <i>Heterosigma akashiwo</i> using ITS sequence

LIANG Bin; CHEN Bin; LUO hao; GUO Hao

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LIANG Bin, CHEN Bin, LUO hao, GUO Hao. Molecular identification of Heterosigma akashiwo using ITS sequence[J]. Haiyang Xuebao, 2008, 30(5): 107-112.

Citation:

LIANG Bin, CHEN Bin, LUO hao, GUO Hao. Molecular identification of Heterosigma akashiwo using ITS sequence[J]. Haiyang Xuebao, 2008, 30(5): 107-112.

LIANG Bin, CHEN Bin, LUO hao, GUO Hao. Molecular identification of Heterosigma akashiwo using ITS sequence[J]. Haiyang Xuebao, 2008, 30(5): 107-112.

Citation:

LIANG Bin, CHEN Bin, LUO hao, GUO Hao. Molecular identification of Heterosigma akashiwo using ITS sequence[J]. Haiyang Xuebao, 2008, 30(5): 107-112.

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Molecular identification of Heterosigma akashiwo using ITS sequence

1.
National Marine Envionmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
2.
National Marine Envionmental Monitoring Center, State Oceanic Administration, Dalian 116023, China;Department of Life Science and Technology, Dalian Fishery University, Dalian 116023, China

Received Date: 2007-11-09
Rev Recd Date: 2008-08-04

Abstract

Abstract

The internal transcribed spacer(ITS)region offive strains which collected from Norway,English Channel,New Zealand,U.S.and Qingdao(China)was amplified with polymerase chain-reaction (PCR)and sequenced.Pairwise distance and percentidentity between ITS sequence of the 5 strains and others retrieved from GenBank were ccalculated.The phylogenetic trees(NJ and ME)were constructed with the ITS sequences which linked to other Raphidophyceae.Results showed that the sequence of 5 strains was identical,which was 538 bp,only one base pair different fromo ther four strains saved in GenBank.Pairwise distance and percent identity are 0.002 and 0.098,respectively.Otherwise completed identical sequences had been found when compared with other strains.The phylogenetic trees constructed by two methods showed totally same results.The Heterosig ma had a close relationship with Chattonella, while was distant fromother two classes.
- Heterosigma akashiwo,
- ITS,
- molecular identification

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References(23)

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