Prediction of the heterosis in hybrids of red snapper <i>Lutjanus erythropterus</i>(♀)&#215;<i>L.sebae</i>(♂)using RAPD

YIN Shao-wu; LIAO Jing-qiu; CHEN Guo-hua; ZHANG Ben

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Article Navigation > Haiyang Xuebao > 2008 > 30(5): 94-99

YIN Shao-wu, LIAO Jing-qiu, CHEN Guo-hua, ZHANG Ben. Prediction of the heterosis in hybrids of red snapper Lutjanus erythropterus(♀)×L.sebae(♂)using RAPD[J]. Haiyang Xuebao, 2008, 30(5): 94-99.

Citation:

YIN Shao-wu, LIAO Jing-qiu, CHEN Guo-hua, ZHANG Ben. Prediction of the heterosis in hybrids of red snapper Lutjanus erythropterus(♀)×L.sebae(♂)using RAPD[J]. Haiyang Xuebao, 2008, 30(5): 94-99.

YIN Shao-wu, LIAO Jing-qiu, CHEN Guo-hua, ZHANG Ben. Prediction of the heterosis in hybrids of red snapper Lutjanus erythropterus(♀)×L.sebae(♂)using RAPD[J]. Haiyang Xuebao, 2008, 30(5): 94-99.

Citation:

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Prediction of the heterosis in hybrids of red snapper Lutjanus erythropterus(♀)×L.sebae(♂)using RAPD

College of Ocean, Hainan University, Haikou 570228, China;Key Laboratory for Tropical Biology Resources of Ministry Education, Haikou 570228, China

Received Date: 2007-07-03
Rev Recd Date: 2008-04-14

Abstract

Abstract

The heterosis of F₁ hy brids(Lutjanus erythropterus ♀ × L.sebae ♂) was predicted with the RAPD method.35 arbitrary primers were screened to amplify the genomic DNA of these three populations of red snapper.190.distinct and highly reproducible RAPD bands with high genetic variability were detected and the frequency of polymorphism was 92.6%.Among the F₁ population,L.erythropterus population and L.sebae population,the values of genetic identitiy were 0.726 3,0.831 9,0.916 8,respectively; the values of Nei's gene diversity were 0.264 0,0.174 5,0.118 4,respectively;the values of Shannons informationindex were 0.384 5,0.256 4,0.171 2,respectively,which all indicated that F₁ populations showed greater genetic diversity than parental populations.Furthermore,the genetic distance between hybrid populations and L.erythropterus ♀ was 0.397 2,0.317 2 between hybrid populations and L.sebae ♂ and 0.854 1 between L.erythropterus ♀ and L.sebae ♂.The data of S,H and I all showed that F₁ owned the potential heterosis.Finally,some species-specific markers were found.
- Lutjanus erythropterus,
- L.sebae,
- hybrid,
- heterosis,
- RAPD

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References(17)

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