Genetic diversity of epiphytic bacteria in <i>Porphyra yezoensis</i>

YANG Rui; FANG Wen-ya; SHAN Yuan-yuan; CHEN Hai-min; SUN Xue; YE Yang-fang

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YANG Rui, FANG Wen-ya, SHAN Yuan-yuan, CHEN Hai-min, SUN Xue, YE Yang-fang. Genetic diversity of epiphytic bacteria in Porphyra yezoensis[J]. Haiyang Xuebao, 2008, 30(4): 161-168.

Citation:

YANG Rui, FANG Wen-ya, SHAN Yuan-yuan, CHEN Hai-min, SUN Xue, YE Yang-fang. Genetic diversity of epiphytic bacteria in Porphyra yezoensis[J]. Haiyang Xuebao, 2008, 30(4): 161-168.

YANG Rui, FANG Wen-ya, SHAN Yuan-yuan, CHEN Hai-min, SUN Xue, YE Yang-fang. Genetic diversity of epiphytic bacteria in Porphyra yezoensis[J]. Haiyang Xuebao, 2008, 30(4): 161-168.

Citation:

YANG Rui, FANG Wen-ya, SHAN Yuan-yuan, CHEN Hai-min, SUN Xue, YE Yang-fang. Genetic diversity of epiphytic bacteria in Porphyra yezoensis[J]. Haiyang Xuebao, 2008, 30(4): 161-168.

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Genetic diversity of epiphytic bacteria in Porphyra yezoensis

Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education, Ningbo 315211, China;Marine Biotechnology Laboratory (NingBo University), Zhejiang Province, Ningbo 315211, China

Received Date: 2007-07-18
Rev Recd Date: 2008-06-02

Abstract

Abstract

Epiphytic bacteria were isolated from Porphyra yezoensis cultivated in the fields or wildly distributed in natural coasts from Nantong (Jiangsu);Qingdao (Shandong) and Wenzhou (Zhejiang) in China,and the conchocelis cultured in Dongtai (Jiangsu) in order to investigate their diversity and the relationship with healthy culture of Porphyra.On the basis of morphological observation,DGGE of 16S rDNA-V3 fragments and 16S rDNA sequence,63 isolated strains were grouped into 21 morphotypes,which had high similarities with strains of 10 genus such as Pseudoalteromonas,Psychrobacter,Bacillus and so on.The analysis showed that the epiphytic bacteria flora in P.yezoensis were relevant with its growth habitats but different from its surroundings.The flora of epiphytic bacteria from different origins,different physiological status (health and disease) and different development stages (thallus and conchocelis) were quite various.The bioactivities of the epiphytic bacteria were measured by the normal tested bacteria and pathogenicbacteria of animal aquaculture or human beings,which showed correlation with the physiological status of their hosts Dominating bacteria from healthy Porphyra belonged to the genus Pseudoalteromonas,which were not found in rotted samples.The significant difference of epiphytic bacterial flora might have some relationships with the healthy growth of P.yezoensis.
- Porphyrayezoensis,
- Epiphytic bacteria,
- Genetic diversity,
- bioactivity

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References(36)

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