Morphological and molecular evidences of <i>Aequorea taiwanensis</i> n. sp. from Taiwan Strait, with mtCOI sequence analysis for genus <i>Aequorea</i>

ZHENG Lian-ming; LIN Yuan-shao; LI Shao-jing; CAO Wen-qing; XU Zhen-zu; HUANG jia-qi

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Article Navigation > Haiyang Xuebao > 2008 > 30(4): 139-146

ZHENG Lian-ming, LIN Yuan-shao, LI Shao-jing, CAO Wen-qing, XU Zhen-zu, HUANG jia-qi. Morphological and molecular evidences of Aequorea taiwanensis n. sp. from Taiwan Strait, with mtCOI sequence analysis for genus Aequorea[J]. Haiyang Xuebao, 2008, 30(4): 139-146.

Citation:

ZHENG Lian-ming, LIN Yuan-shao, LI Shao-jing, CAO Wen-qing, XU Zhen-zu, HUANG jia-qi. Morphological and molecular evidences of Aequorea taiwanensis n. sp. from Taiwan Strait, with mtCOI sequence analysis for genus Aequorea[J]. Haiyang Xuebao, 2008, 30(4): 139-146.

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Morphological and molecular evidences of Aequorea taiwanensis n. sp. from Taiwan Strait, with mtCOI sequence analysis for genus Aequorea

Department of Oceanography, Xiamen University, Xiamen 361005, China

Received Date: 2007-07-03
Rev Recd Date: 2008-05-23

Abstract

Abstract

Aequoreataiwanensis n.sp.,a new species of Aequorea:from Minnan-Taiwan Shoal fishing ground upwelling region was described in this paper.Mitochondrial cytochrome oxidase subunit Ⅰ (mtCOI) gene fragments of A.taiwanensis,A.papillata and A.conica was successfully amplified by universal primers,and obtained 538 bp sequences.Sequence divergence and genetic distance was analysed based on the mtCOI gene sequences.Both morphological and molecular data supported that A.taiwanensis n.sp is a valid species.The mtCOI sequences from the three speices of genus Aequorea showed high variation frequency,with sequence divergences ranging from 9.10% to 11.9% and pairwise genetice distances ranging from 0.097 to 0.130.mtCOI sequence analysis proved to be diagnostic molecular systematic characteristics for accurate identification and discrimination of the species of Aequorea or its population,and will be used to resolved evolutionary relationships among species of Aequorea.We suggested that a difference of from 10 to 20% between mtCOI sequences indicated species-level divergence among cogeneric species in Hydromedusa.Morphological description of Aequoreataiwanensis n.sp.:Usually 25 mm wide.Umbrellaplatter than a hemisphere,lens-shaped,with solid jelly,jelly thicker in centre,thinning gradually and evenly towards umbrella margin;manubrium broad and large,about one-half diameter of umbrella;lateral walls very extensive,with transparent lines radiating from ends of radial canals to mout-hlips;mouth-lips elongated,slender-shaped,usually approximately as numerous as radial canals.Radial canals straight and narrow,usually 90~102 in number.Ring canal narrow;gonads linear,extending along almost whole length of radial canals.With 12 marginal tent acles,with elongated conical bases narrowing suddenly distally,with a short adaxial excretory papillae,scattering black pigments.With 3~5 small,marginal bulbs between marginal tentacles with adaxial excretory papillae,but without black pig ments.With one statocysts between successive radial canals,each with 1~2 concretions,velum narrow.
- Aequorea,
- new species,
- Aequoreataiwanensis n. sp.,
- mtCOI,
- sequence,

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References(35)

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