Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus

XUE Liang-yi; LI Ting; YANG Qiao-yi; XIAO Zhang-kui

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XUE Liang-yi, LI Ting, YANG Qiao-yi, XIAO Zhang-kui. Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus[J]. Haiyang Xuebao, 2008, 30(3): 95-101.

Citation:

XUE Liang-yi, LI Ting, YANG Qiao-yi, XIAO Zhang-kui. Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus[J]. Haiyang Xuebao, 2008, 30(3): 95-101.

XUE Liang-yi, LI Ting, YANG Qiao-yi, XIAO Zhang-kui. Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus[J]. Haiyang Xuebao, 2008, 30(3): 95-101.

Citation:

XUE Liang-yi, LI Ting, YANG Qiao-yi, XIAO Zhang-kui. Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus[J]. Haiyang Xuebao, 2008, 30(3): 95-101.

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Cloning and tissue expression analysis of the myostatin gene in the red drum,Sciaenops ocellatus

1.
College of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, China;Education Ministry Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo, 315211, China
2.
College of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, China

Received Date: 2006-12-18
Rev Recd Date: 2007-06-28

Abstract

Abstract

Myostatin is a negative regulator of skeletal muscle growth and has a potential application in aquaculture.The characterization of the myostatin gene and its expression in the red drum fish,Sciaenops ocellatus,were reported.The myostat in gene possessed three exons encoding 376 amino acids.The cDNA was 1750 bp long with a 108 bp 5'-UT R and a 511 bp 3'-UTR.Two micr osat ellite sequences,(TC)₄CC(TC)₄CT(TC)₄ and(AC)₇,existed in intron Ⅰand 3'-UTR,respectively.The deduced amino acid sequence was highly conserved,and had more than 90% identical to Umbrinacirrosa,Sparus aurata,Morone saxatilis,Morone amer icana,and Morone chrysops proteins.The myostatin of the red drum had a putative amino terminal signal sequence(residues 1-22),a TGF-β propeptide domain(residues 41~256),a RXXR proteolytic processing site(RARR,residues 264~267,matching the RXXR consensus site),and a TGF-B domain(residues 282~376).There were 13 conserved cysteine residues in the red drum myostatin,nine of which are common to all TGF-β superfamily members.The most conserved region of vertebrate myostatins is the TGF-β domain,which was the mature bioactive domain of the myostat in protein.In the examined tentissues,the myostatingene was expressed only in three,the skeletal muscle,brain and eye.The strongest expression was in the skeletal muscle.
- Sciaenop socellatus,
- myostatin,
- cloning,
- tissue expression

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References(31)

References

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