Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
Biology Department, Qingdao University of Science&Technology, Qingdao 266042, China;Key Lab of Anal.Tech.and Std.of Chinese Medicines, First Institute of Oceanography, State Oceanic Adminis-tration, Qingdao 266061, China
2.
Key Lab of Anal.Tech.and Std.of Chinese Medicines, First Institute of Oceanography, State Oceanic Adminis-tration, Qingdao 266061, China
3.
Biology Department, Qingdao University of Science&Technology, Qingdao 266042, China
4.
Peking University, Health Sciena Center, Beijing 100083, China
A marine fungus Penicillium crustosum 2A4-Z15,which has strong inhibition activity,w as separated from South China Sea and identified.It was treated by four biotic elicitors and five abiotic elicitors.Four biotic elicitors originated from different marine microorganism including Bacillus sp.,Penicillium sp.,Trichorderma sp.and Fucarium sp..Five abiotic elicitors included three aminoacids used as precursors and two inhibitors.The effect of different elicitors on alkaloid metabolism of the marine fung us was studied.The results show that different elicitors have different effecton alkaloid metabolism and biomass.Generally speaking,biotic elicitors have evidently positive influenceon them.Aminoacids added as precursors can show positive influence only with proper kind and concentration.Inhibitors tested both show negative influence.Alkaloid metabolism can be enhanced by 44.1% by the marine bacterial elicitor with a 10μg/cm3 concent ration added at logarithmic period
YU R M,WANG Y,MA L,et al.Production of Active Alkaloids by Elicited Papaver Nudicaule Cell Suspension Cultures[J].Pharmaceutical Biotechnology,2003,10(4):2181222.
MAN J R,GAO P P.Effect of several elicitors on sclerotia biomass and carotenoid yield of PeniciUium sp.PT95 during solid-state fermentation of corn meal[J].World Journal of Microbiology &.Biotechnology,2002,18:367-371.
WHITMER S,HEIJDEN R,VETPOORTE R.Effect of precursor feeding on alkaloid accumulation by a strictosidine synthase over-expressing transgenic cell line S1 of Catharanthus roseus[J].Plant Cell,Tissue and Organ Culture,2002,69:85-93.
MORENO-VALENZUELA O A,MINERO-GARCIA Y,CHAN W,et al.Increase in the indole alkaloid production and its excretion into the culture medium by calcium antagonists in Catharanthus roseus hairy roots[J].Biotechnology Letters,2003,25:1345-1349.