White spot syndrome virus envelope protein VP124 is involved in the virus infection

ZHU Yan-bing; WU Cheng-lin; YANG Feng

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ZHU Yan-bing, WU Cheng-lin, YANG Feng. White spot syndrome virus envelope protein VP124 is involved in the virus infection[J]. Haiyang Xuebao, 2008, 30(1): 135-139.

Citation:

ZHU Yan-bing, WU Cheng-lin, YANG Feng. White spot syndrome virus envelope protein VP124 is involved in the virus infection[J]. Haiyang Xuebao, 2008, 30(1): 135-139.

ZHU Yan-bing, WU Cheng-lin, YANG Feng. White spot syndrome virus envelope protein VP124 is involved in the virus infection[J]. Haiyang Xuebao, 2008, 30(1): 135-139.

Citation:

ZHU Yan-bing, WU Cheng-lin, YANG Feng. White spot syndrome virus envelope protein VP124 is involved in the virus infection[J]. Haiyang Xuebao, 2008, 30(1): 135-139.

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White spot syndrome virus envelope protein VP124 is involved in the virus infection

1.
School of Biotechnology, Jimei University, Xiamen 361021, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2006-06-28
Rev Recd Date: 2007-06-18

Abstract

Abstract

White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses for shrimp farming.In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually.And then they were injected intramuscularly into crayfish to conduct in vivo neutralizationassays.The results showed that for groups injected with virions only and groups injected with virions and the VP124 antiserum mixture, the crayfish mortalities were 100% and 60% at 8 day spost-infection, individually.The virus infection could be neutralized by antibody against the envelope protein VP124.Quantitative PCR were used to further investigate the influence of three antisera above described on the virus infection.The results showed that the VP124 antiserum couldrestrainthe propagation of WSSV in cray fish.All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.
- white spot syndrome virus,
- envelope protein VP124,
- antibody,
- infection,
- neutralization assay

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References(16)

References

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