Screening of antagonistic bacteria against pathogenic <i>Vibrio parahaemolyticus</i> of <i>Pseudosciaena crocea</i>

GUO Guo-jun; QIN Yin-xue; CHEN Qiang; ZOU Wen-zheng; YAN Qing-pi

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Article Navigation > Haiyang Xuebao > 2008 > 30(1): 127-134

GUO Guo-jun, QIN Yin-xue, CHEN Qiang, ZOU Wen-zheng, YAN Qing-pi. Screening of antagonistic bacteria against pathogenic Vibrio parahaemolyticus of Pseudosciaena crocea[J]. Haiyang Xuebao, 2008, 30(1): 127-134.

Citation:

GUO Guo-jun, QIN Yin-xue, CHEN Qiang, ZOU Wen-zheng, YAN Qing-pi. Screening of antagonistic bacteria against pathogenic Vibrio parahaemolyticus of Pseudosciaena crocea[J]. Haiyang Xuebao, 2008, 30(1): 127-134.

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Screening of antagonistic bacteria against pathogenic Vibrio parahaemolyticus of Pseudosciaena crocea

Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University), Fujian 361021, China

Received Date: 2006-07-07
Rev Recd Date: 2007-01-16

Abstract

Abstract

104 strains of antagonistic bacteria against pathogenic Vibrio parahaemolyticus were isolated from dominant flor a of Pseudosciaena crocea intestine by dot-inoculating method.The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra, 3 strains isolated from manganic nutrientagar plate had advantage on growth.The utilization of carbon source of three strains(R22, Y58 and J312) which had a wide antibacterial spectra and growth advantages were mensurated by BIOLOG.The results showed that: all of the 3 strains had wider carbon-source spectra; they could utilize saccharide, organic acid and amino acid; there were no severity competition on nutriment between antagonistic bacteria and marine fish.All results above indicate that these three strains of antagonistic bacteria accord with the properties of probiotics, which should be further studied.16S rRNA gene homology of the three strains were analyzed in Genebank, and the results showed that strains had homology more than 99% with R22, Y58 and J312 were Vibrio, Bacillus and Pseudomonas respectively.Results of further biochemical identification showed that: R22 was V.alginolyticus, J312 was P.fluorescens, while Y58 could not be identified by bio Merieux.Although antagonistic bacteria isolated (strain R22, Y58, J312) measure up to probiotics for their antagonistic effect, fast growth and carbon source utlity, their security to fish and application effect should be confirmed before they can be use as probiotics.
- Pseudosciaena crocea,
- antagonistic bacteria,
- antibacterial spectra,
- growth curves,
- 16S rRNA,
- carbon source utility

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References(22)

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